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ERVFRD-1 Antibody

  • 货号：
    CSB-PA352689ESR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
      All lanes: ERVFRD-1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 60 kDa
      Observed band size: 60 kDa
    • Immunofluorescence staining of Hela cell with CSB-PA352689LA01HU at 1:160, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The rabbit was immunized with recombinant human Syncytin-2 protein (16-250aa) to produce the ERVFRD-1 polyclonal antibody, which is an IgG isoform and exhibits a liquid form. This ERVFRD-1 antibody underwent antigen affinity purification. This ERVFRD-1 antibody shows reactivity with the human ERVFRD-1 protein.

    The specificity of this ERVFRD-1 antibody has been validated in ELISA, WB, and IHC. The ERVFRD-1 polyclonal antibody can be used to detect and study the expression of the ERVFRD-1 protein as well as to visualize the distribution of the ERVFRD-1 protein. ERVFRD-1 is a critical protein for placental development and the healthy development of the fetus during pregnancy.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ERVFRD-1 Polyclonal antibody
  • Uniprot No.：
    P60508
  • 基因名：
    ERVFRD-1
  • 别名：
    EFRD1_HUMAN antibody; Endogenous retrovirus group FRD member 1 antibody; Envelope polyprotein antibody; ERVFRD-1 antibody; FLJ41944 antibody; FLJ90611 antibody; GLLL6191 antibody; HERV FRD 6p24.1 provirus ancestral Env polyprotein antibody; HERV FRD antibody; HERV FRD provirus ancestral Env polyprotein antibody; HERV-FRD antibody; HERVFRD antibody; HERVFRD provirus ancestral Env polyprotein antibody; MGC87585 antibody; SU antibody; Syncytin 2 antibody; Syncytin B antibody; Syncytin-2 antibody; TM antibody; Transmembrane protein antibody; UNQ6191 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Syncytin-2 protein (16-250AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IF 1:100-1:300
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. The interaction with MFSD2A is apparently important for this process.; Endogenous envelope proteins may have kept, lost or modified their original function during evolution but this one can still make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein mediates receptor recognition, while the transmembrane protein anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.
  • 基因功能参考文献：
    1. genetic predisposition in ERVFRDE-1 may be associated with an increased risk of preeclampsia. This polymorphism is possibly involved in the regulation of syncytin-2 expression in preeclamptic placenta. PMID: 29750965
    2. N-glycans at residues 133, 312, 332 and 443 of syncytin-2 are required for optimal fusion induction, and that single-nucleotide polymorphism C46R, N118S, T367M, R417H, V483I and T522M can alter the fusogenic function of syncytin-2. PMID: 26853155
    3. Decreased syncytin-2 and MFSD2 proteins in gestational diabetic placentas might cause abnormal syncytiotrophoblast formation and possibly be involved in the pathology PMID: 26875564
    4. ERVWE1, ERVFRDE1 and ERV3 transcription was down-regulated in hydatidiform moles and gestational trophoblastic neoplasia. PMID: 26992684
    5. These results thereby demonstrate that induced expression of Syncytin-2 is highly dependent on the interaction of bZIP-containing transcription factors to a CRE/AP-1 motif and that this element is important for the regulation of Syncytin-2 expression PMID: 25781974
    6. MFSD2a, the Syncytin-2 receptor, is important for trophoblast fusion. PMID: 23177091
    7. results further highlighted the existence of a correlation between the extent of the decrease in the expression levels of both syncytins 1 and 2 fusogenic proteins and the degree of severity of preeclampsia symptoms PMID: 21493955
    8. Analysis of non-spliced ERVFRDE1 mRNAs and env mRNAs detected efficient splicing of endogenously expressed RNAs in trophoblastic but not in non-placental cells. PMID: 21771862
    9. The crystal structure of a central fragment of syncytin 2 "fossil" ectodomain is reported, allowing a remarkable superposition with the structures of the corresponding domains of present-day infectious retroviruses PMID: 16140326
    10. immunolocalized only in the villous trophoblast of the chorionic villi, at the level of cytotrophoblastic cells PMID: 16714059
    11. findings show that in both humans and mice, one of the two syncytins (human syncytin-2 and mouse syncytin-B) is immunosuppressive and, rather unexpectedly, the other (human syncytin-1 and mouse syncytin-A) is not PMID: 18077339
    12. Syncytin 2 expression illustrates the abnormal trophoblast differentiation observed in placenta of fetal T21-affected pregnancies. PMID: 18215254
    13. expression decreased in preeclamptic placentas; may function as a second fusogenic protein for placental cell fusion PMID: 18650494
    14. These results highlight the importance of Syncytin-2 in BeWo and primary human trophoblast cell fusion. PMID: 19616006

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  • 亚细胞定位：
    Virion.; [Surface protein]: Cell membrane; Peripheral membrane protein.; [Transmembrane protein]: Cell membrane; Single-pass membrane protein.
  • 蛋白家族：
    Gamma type-C retroviral envelope protein family, HERV class-I FRD env subfamily
  • 组织特异性：
    Expressed at higher level in placenta. Expressed at lower level in adrenal, bone marrow, brain, breast, colon, kidney, lung, ovary, peripheral blood lymphocytes, prostate, skin, spleen, testis, thymus, thyroid, trachea.
  • 数据库链接：

    HGNC: 33823

    OMIM: 610524

    KEGG: hsa:405754

    STRING: 9606.ENSP00000420174

    UniGene: Hs.631996