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  4. EPS8 Antibody

EPS8 Antibody

  • 货号：
    CSB-PA007751GA01HU
  • 规格：
    ￥3,900
  • 其他：

产品详情

  • Uniprot No.：
    Q12929
  • 基因名：
    EPS8
  • 别名：
    Epidermal growth factor receptor kinase substrate 8 antibody; Epidermal growth factor receptor pathway substrate 8 antibody; EPS 8 antibody; EPS8 antibody; EPS8_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Human EPS8
  • 免疫原种属：
    Homo sapiens (Human)
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes.
  • 基因功能参考文献：
    1. Data showed that chronic myeloid leukemia (CML) patients expressed a higher level of EPS8 mRNA in bone marrow mononuclear cells. Functional results revealed that EPS8 regulated multiple biological functions such as proliferation, apoptosis, cell cycle, drug sensitivity of CML cells possibly by mediating the regulation of the BCR-ABL/AKT/mTOR signalling pathway. PMID: 29192326
    2. These results indicate that plasma-membrane-associated PTK6 phosphorylates Eps8, which promotes cell proliferation, adhesion, and migration PMID: 28214294
    3. Eps8/Abi1/Sos1 tricomplex acts as a key molecular switch altering the balance between Rac1 and Rho activation; its presence or absence in pancreatic ductal adenocarcinoma cells modulates alphavbeta6-dependent functions, resulting in a pro-migratory (Rac1-dependent) or a pro-TGF-beta1 activation (Rho-dependent) functional phenotype PMID: 28608476
    4. Immunohistochemistry revealed that Eps8 was significantly increased in cervical cancer specimens compared with squamous intraepithelial lesion and normal cervical tissues. Additionally, it was revealed that Eps8 expression not only correlated with cervical cancer progression, but also exhibited a close correlation with the epithelialmesenchymal transition (EMT) markers, Ecadherin and vimentin. PMID: 27573546
    5. Eps8 is required for continuous membrane blebbing. PMID: 26976596
    6. Erk activity promotes actin bundling by Eps8 to enhance cortex tension and drive the bleb-based migration of cancer cells under non-adhesive confinement. PMID: 26163656
    7. Eps8 is a crucial mediator of Src- and FAK-regulated processes. PMID: 25359883
    8. Eps8 is overexpressed in human breast cancers, possibly by regulating ERK signaling, MMP9, p53 and EMT-like transition to affect breast cancer cell growth, migration and invasion. PMID: 25333707
    9. EPS8, as MDR1 and WT1, may be a clinically valuable biomarker for assessing the outcome of ALL patients. PMID: 25843487
    10. These results indicate that employing the native and modified epitopes identified here in Eps8-based immunotherapy for HLA-A2.1 positive cancer patients may result in efficient anticancer immune responses for diverse tumor types. PMID: 25376540
    11. The ITSN2 interacts with Eps8 and stimulates the degradation of Eps8 proteins. PMID: 22449706
    12. EPS8 is an F-actin capping and bundling protein. Mutant mice lacking EPS8 (Eps8-/- mice), which is present in the hair bundle, the sensory antenna of the auditory sensory cells that operate the mechano-electrical transduction PMID: 24741995
    13. Eps8 functions as a key coordinator in the interplay between FGFR signalling and trafficking. PMID: 23203811
    14. results suggest that Eps8 may serve as a prognostic factor of responsiveness to chemotherapy in AML patients PMID: 24409660
    15. Novel binding partners and differentially regulated phosphorylation sites clarify Eps8 as a multi-functional adaptor. PMID: 23626693
    16. Eps8 is frequently expressed in OSCC. The aberrant expression of Eps8 closely correlated with poor survival in patients with OSCC. PMID: 22897151
    17. We found that Eps8 mediates cell proliferation and survival of glioma cells, at least in part, by affecting phosphorylated ERK and Akt/beta-catenin activities. PMID: 23229386
    18. Identify Fbxw5-driven fluctuation of Eps8 levels as an important mechanism that contributes to cell-shape changes during entry into-and exit from-mitosis. PMID: 23314863
    19. The loss of EPS8 expression in colorectal adenomas and carcinomas suggests that down regulation of this gene contributes to the development of a subset of colorectal cancers. PMID: 22876043
    20. silencing of the protein by siRNA abrogated the migratory and invasive capacity of three different glioblastoma cell lines both in 2-dimensional and 3-dimensional in vitro assays. PMID: 22683923
    21. Eps8 is a key regulator of the LPS-stimulated TLR4-MyD88 interaction and contributes to macrophage phagocytosis PMID: 22493489
    22. Studied generation of filopodia with regards to the dynamic interaction established by Eps8, IRSp53 and VASP with actin filaments. PMID: 21814501
    23. critical role for JNK2 and EPS8 in receptor tyrosine kinase signaling and trafficking to convey distinctly different effects on cell migration. PMID: 21357683
    24. Study implicates that the integrity of SOS1/EPS8/ABI1 tri-complex is a determinant of ovarian cancer metastasis. PMID: 21118970
    25. IRSp53, through its interaction with Eps8, not only affects cell migration but also dictates cellular growth in cancer cells. PMID: 20418908
    26. Overexpression of EPS8 induced expression of the chemokine ligands CXCL5 and CXCL12 in a FOXM1-dependent manner, which was blocked by LY294002 or a dominant-negative form of AKT PMID: 20351091
    27. Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells PMID: 20184880
    28. Involved in the growth factor-controlled regulation of cell proliferation and differentiation in the seminiferous epithelium. PMID: 15273867
    29. These results support a model whereby the synergic bundling activity of the IRSp53-Eps8 complex, regulated by Cdc42, contributes to the generation of actin bundles, thus promoting filopodial protrusions. PMID: 17115031
    30. Eps8 is essential for actin dynamics and cell interactions, independent of Eps8-like gene products. PMID: 17537571
    31. These findings implicate the involvement of Eps8 in chemoresistance and show its importance in prognosis of cervical cancer patients. PMID: 18566210
    32. overexpression of EPS8 in HN4 cells was sufficient to induce growth of non-tumorigenic cells in orthotopic transplantation assays. EPS8 expression in samples of squamous cell carcinoma showed variable expression levels and paralleled expression of MMP-9 PMID: 19008210
    33. A role of Eps8 in amplifying growth factor receptor signaling in human pituitary tumors to promote proliferation and cell survival. PMID: 19116338
    34. Results show that alphavbeta6- and alpha5beta1-integrin-dependent activation of Rac1 was mediated through Eps8. PMID: 19448673
    35. the crystal structures of human LanCL1, both free of and complexed with glutathione, revealing glutathione binding to a zinc ion at the putative active site formed by conserved GxxG motifs PMID: 19528316

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  • 相关疾病：
    Deafness, autosomal recessive, 102 (DFNB102)
  • 亚细胞定位：
    Cytoplasm, cell cortex. Cell projection, ruffle membrane. Cell projection, growth cone. Cell projection, stereocilium. Cell junction, synapse, synaptosome.
  • 蛋白家族：
    EPS8 family
  • 组织特异性：
    Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • 数据库链接：

    HGNC: 3420

    OMIM: 600206

    KEGG: hsa:2059

    STRING: 9606.ENSP00000281172

    UniGene: Hs.591160