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ENTPD1 Antibody

  • 货号：
    CSB-PA007690LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HL-60 whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate
      All lanes: ENTPD1 antibody at 2.6μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 58, 59, 35, 43, 47, 60 kDa
      Observed band size: 58, 43 kDa
    • IHC image of CSB-PA007690LA01HU diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA007690LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The ENTPD1 polyclonal antibody from CUSABIO is a highly specific and affinity-purified antibody that is directed against the human ENTPD1 protein. The immunogen used to generate this ENTPD1 antibody is the recombinant human ENTPD1 protein (316-474AA). It has been validated for use in ELISA, WB, IHC, and IF. This antibody has a protein G purification, and its purity is greater than 95%.

    The ENTPD1 protein, also known as CD39, mainly functions to regulate ectonucleotidase activity. It plays an important role in regulating inflammation, immune responses, and vascular function. Dysregulation of ENTPD1 activity has been implicated in a variety of diseases, including inflammation, thrombosis, and cancer.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ENTPD1 Polyclonal antibody
  • Uniprot No.：
    P49961
  • 基因名：
  • 别名：
    ATPDase antibody; CD 39 antibody; CD39 antibody; CD39 antigen antibody; DKFZp686D194 antibody; DKFZp686I093 antibody; Ecto apyrase antibody; Ecto ATP diphosphohydrolase antibody; Ecto-apyrase antibody; Ecto-ATP diphosphohydrolase 1 antibody; Ecto-ATPase 1 antibody; Ecto-ATPDase 1 antibody; Ectonucleoside triphosphate diphosphohydrolase 1 antibody; ENTP1_HUMAN antibody; ENTPD 1 antibody; ENTPD1 antibody; FLJ40921 antibody; FLJ40959 antibody; Lymphoid cell activation antigen antibody; NTPDase 1 antibody; NTPDase1 antibody; SPG64 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 1 protein (316-474AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ENTPD1 Antibody (CSB-PA007690LA01HU)，的标记方式是Non-conjugated。对于ENTPD1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA007690LB01HU ENTPD1 Antibody, HRP conjugated ELISA
    FITC CSB-PA007690LC01HU ENTPD1 Antibody, FITC conjugated
    Biotin CSB-PA007690LD01HU ENTPD1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.
  • 基因功能参考文献：
    1. Changes in the local expression and activity of CD39 and CD73 in calcified valves suggest their potential role in calcific aortic valve disease. PMID: 30056298
    2. Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors. PMID: 30006565
    3. CD39 expression and activity is attenuated in lung tissue of chronic obstructive pulmonary disease patients. PMID: 29807526
    4. monocyte-derived macrophages from ankylosing spondylitis patients expressed reduced levels of CD39 mRNA compared to those from healthy controls PMID: 29524036
    5. expression in primary lesions and metastatic lymph nodes seems to identify patients at high risk in squamous cell carcinoma of the head and neck PMID: 29172836
    6. ur results demonstrate that CD39 is upregulated on conventional CD4+ and CD8+ T cells at sites of acute infection and inflammation, and that CD39 dampens responses to bacterial infection. PMID: 29742141
    7. this paper shows that simultaneous overexpression of human E5NT and ENTPD1 protects porcine endothelial cells against H2O2-induced oxidative stress and cytotoxicity in vitro PMID: 28389406
    8. The peripheral blood mononuclear cells (PBMC) from the transgenic pigs were more resistant to lysis by pooled complement-preserved normal human serum than that from wild type (WT) pig. Accordingly, GGTA1 mutated piglets expressing hCD39 will provide a new organ source for xenotransplantation research PMID: 27830476
    9. Phosphoantigens (pAgs) induced expression of the ecto-ATPase CD39, which, however, not only hydrolyzed ATP but also abrogated the gammadelta T cell receptor (TCR) agonistic activity of self and microbial pAgs. PMID: 27346340
    10. These studies showed that the G allele of rs3176891 marks a haplotype associated with increased clotting and platelet aggregation attributable to a promoter variant associated with increased transcription, expression, and activity of NTPDase1. PMID: 28302652
    11. Transgenic expression of human CD39 is associated with increased renal fibrosis after ischemia in mice. PMID: 28198766
    12. CD39 overexpression protects against cerebral ischemia in a transgenic mouse model. PMID: 28377485
    13. Ablation of CD73 minimally effects in vivo thrombosis, but increased CD39 expression on hematopoietic-derived cells, especially monocytes, attenuates in vivo arterial thrombosis. PMID: 27417582
    14. Data show that Th17(CD39+) cells are markedly diminished and fail to generate AMP/adenosine, thereby limiting control of both target cell proliferation and IL-17 production in juvenile autoimmune liver disease (AILD). PMID: 27210814
    15. concluded that CD39 and CD73 are molecular targets for the development of drugs for ALF patients care PMID: 27899277
    16. Oxidized low density lipoproteins modulate CD39 and CD73 activity in the endothelium. PMID: 27906627
    17. this study shows that T-cell expression of CD39 was higher in acute exacerbations of chronic obstructive pulmonary disease patients than stable COPD patients or healthy controls PMID: 27430193
    18. Pulmonary CD39 expression and activity are increased in COPD. Following acute cigarette smoke exposure CD39 was upregulated in BALF cells in smokers. PMID: 26541524
    19. This study aimed to investigate the activities of purinergic system ecto-enzymes present on the platelet surface as well as CD39 and CD73 expressions on platelets of sickle cell anemia treated patients. PMID: 27044834
    20. Increased inducibility of CD39 after activation may contribute to the impaired vaccine response with age. PMID: 26832412
    21. The altered function and expression of P2X7 and ART1 in the human CD39+ Treg or CD39- Treg cells could participate in the resistance against cell death induced by ATP or NAD. PMID: 26307000
    22. Despite the increased level of NTPDase1 and NTPDase3 mRNA expression in chondrogenically induced MSCs, their activity toward ATP remains quite low. PMID: 26018728
    23. the expression of CD39 on Treg cells and also in CD4(+)IL-17(+) cells from T2D patients is related to hyperglycemia as well as to overweight and obesity and therefore may participate as a modulator of the effector capacity of Th17 cells. PMID: 26386144
    24. We suggest modulation of human Th17 responsiveness by CD39 and CD161 and describe novel molecular mechanisms integrating elements of both extracellular nucleotide and sphingolipid homeostasis--{REVIEW} PMID: 26059452
    25. Findings provide insights into Tc1-mediated IFNgamma responses and ROS generation and link these pathways to CD39/adenosine-mediated effects in immunological disease. PMID: 26549640
    26. the current study revealed that malignant epithelial cells of human rectal adenocarcinoma strongly express CD39 that may play a potential role in the tumor invasion and metastasis. PMID: 26113408
    27. role of CD73 and CD39 ectonucleotidases in T cell differentiation PMID: 26226423
    28. this study demonstrates that the expression of CD39 in Tregs is primarily genetically driven, and this may determine interindividual differences in the control of inflammatory responses. PMID: 25640206
    29. these data establish CD39 as a regionalized regulator of atherogenesis that is driven by shear stress. PMID: 26121751
    30. our data indicate that T-cell CD39 expression may identify subsets of patients with B-CLL with an unfavorable clinical outcome. PMID: 24684231
    31. The Na-K-2Cl cotransporter was downregulated by high-sodium diet in wild-type mice, but it increased in transgenic mice overexpressing human CD39 PMID: 25877509
    32. apelin, a known regulator of pulmonary vascular homeostasis, can potentiate the activity of CD39 both in vitro and in vivo PMID: 25820525
    33. Blackcurrant leaf extract increases endothelial cell NOS and CD39 levels in a concentration dependent manner. PMID: 25407137
    34. Data show that Rubus leave extracts significantly increased CD39 antigen NTPDase 1 ecto-ATP diphosphohydrolase 1 (CD39/NTPDase-1) expressions and decreased ATPDase activities. PMID: 25034034
    35. Low expression of CD39(+) /CD45RA(+) on regulatory T cells (Treg ) cells in type 1 diabetic children in contrast to high expression of CD101(+) /CD129(+) on Treg cells in children with coeliac disease. PMID: 25421756
    36. by regulating ATP availability at the cardiac mast cell surface surface, CD39 modulates local renin release and thus, renin-angiotensin system activation, ultimately exerting a cardioprotective effect PMID: 25318477
    37. Our data suggests that human bone marrow-derived mesenchymal stem cells can effectively suppress immune responses of the Th17 cells via the CD39-CD73-mediated adenosine-producing pathway PMID: 24043462
    38. ecto-nucleotidases CD39 and CD73 are expressed in human endometrial tumors PMID: 24707115
    39. FOXP3(+) CD39(+) Treg cells are enriched at the site of inflammation, do not produce proinflammatory cytokines, and are good suppressors of many effector T-cell functions including production of IFN-gamma, TNF, and IL-17F but do not limit IL-17A secretion PMID: 24990235
    40. CD39 and CD161 modulate human Th17 responses in CD through alterations in purinergic nucleotide-mediated responses and ASM catalytic bioactivity, respectively. PMID: 25172498
    41. The present findings suggest the existence of an endogenous anti-tissue destructive mechanism in gingival tissue via the CD39-adenosinergic axis. PMID: 23941770
    42. Results show the interplay between promoter SNPs of CD39 and FAM134B results in an intercellular epistasis which influences the risk of a complex inflammatory disease. PMID: 24970562
    43. The data indicates that glioma-derived CD73 contributes to local adenosine-mediated immunosuppression in synergy with CD39 from infiltrating CD4(+)CD39(+) T lymphocytes in human malignant gliomas. PMID: 23737488
    44. These data identify CD39 as a novel marker of human regulatory CD8(+) T cells and indicate that CD39 is functionally involved in suppression by CD8(+) Treg cells PMID: 23606272
    45. CD39 counteraction inhibits the suppression activity of CD8+ Treg (both from peripheral blood and tumor microenvironment) suggesting that CD39-mediated inhibition constitutes a prevalent hallmark of their function PMID: 23359087
    46. hCD39 expressed by circulating leukocytes and intrinsic renal cells limits innate AN injury. PMID: 22684996
    47. The expression of ENTPD1 and ecto-adenosine deaminase in lymphocytes of Chagase disease patients are reported. PMID: 22846899
    48. We demonstrate for the first time increased CD39 expression and function on circulating microparticles in patients with IPAH. PMID: 22792409
    49. identified hitherto unrecognized soluble forms of AK1 and NTPDase1/CD39 that contribute in the active cycling between the principal platelet-recruiting agent ADP and other circulating nucleotides PMID: 22637533
    50. These findings not only suggest that CD39 Treg cells may be involved in hepatitis B virus disease progression but also identify CD39 Tregs as a dynamic immune regulatory cell population that may represent a new target of immunomodulatory therapeutic interventions. PMID: 22489829

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  • 相关疾病：
    Spastic paraplegia 64, autosomal recessive (SPG64)
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    GDA1/CD39 NTPase family
  • 组织特异性：
    Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only.
  • 数据库链接：

    HGNC: 3363

    OMIM: 601752

    KEGG: hsa:953

    STRING: 9606.ENSP00000360250

    UniGene: Hs.576612