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EIF5B Antibody

  • 货号：
    CSB-PA080048
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    O60841
  • 基因名：
    EIF5B
  • 别名：
    DKFZp434I036 antibody; eIF 5B antibody; eIF-5B antibody; Eif5b antibody; Eukaryotic translation initiation factor 5B antibody; FLJ10524 antibody; IF2 antibody; IF2P_HUMAN antibody; KIAA0741 antibody; Translation initiation factor IF 2 antibody; Translation initiation factor IF-2 antibody; Translation initiation factor IF2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the C-terminal region of Human eIF5B.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:40000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
  • 基因功能参考文献：
    1. eIF5B promoted hepatocellular carcinoma cell proliferation and migration in vitro and in vivo partly through increasing ASAP1 expression. PMID: 27694689
    2. eIF5B silencing provides a negative feedback to deactivate MAPK signaling PMID: 27959964
    3. results indicate that the interactions between eIF1A and eIF5B are being continuously rearranged during translation initiation; presentation of a model how the dynamic eIF1A/eIF5B interaction network can promote remodeling of the translation initiation complexes, and the roles in the process played by intrinsically disordered protein segments PMID: 27325746
    4. eIF5B overexpression promotes maturation of G0-like immature oocytes and causes cell death, an alternative to G0, in serum-starved THP1 cells. PMID: 25261552
    5. The s show that the cleavage of initiation factor eIF5B during enteroviral infection, along with the viral internal ribosome entry site, plays a role in mediating viral translation under conditions that are nonpermissive for host cell translation. PMID: 21697471
    6. determination of binding site on eukaryotic initiation factor 1A PMID: 12569173
    7. Transfected nuclear factor 2 trans activates the IF2 promoter in a cell line. PMID: 17161026
    8. binding of eIF5B might induce conformational changes in both subunits, with ribosomal segments wrapping around the factor PMID: 17568775
    9. 3Cpro-mediated cleavage of eIF5B may thus play an accessory role in the shutoff of translation that occurs in enterovirus-infected cells. PMID: 18572216

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, IF-2 subfamily
  • 数据库链接：

    HGNC: 30793

    OMIM: 606086

    KEGG: hsa:9669

    STRING: 9606.ENSP00000289371

    UniGene: Hs.158688