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EIF3G Antibody

  • 货号：
    CSB-PA03099A0Rb
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA03099A0Rb diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA03099A0Rb diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA03099A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) EIF3G Polyclonal antibody
  • Uniprot No.：
    O75821
  • 基因名：
    EIF3G
  • 别名：
    44kDa antibody; Anti EIF3S4 antibody; eIF 3 delta antibody; eIF 3 RNA binding subunit antibody; eIF-3 RNA-binding subunit antibody; eIF-3-delta antibody; eIF3 delta antibody; eIF3 p42 antibody; eIF3 p44 antibody; eIF3g antibody; EIF3G_HUMAN antibody; EIF3S4 antibody; EIF3S4 antibody antibody; EIF3S4; formerly antibody; Eukaryotic translation initiation factor 3 antibody; Eukaryotic translation initiation factor 3 RNA binding subunit antibody; Eukaryotic translation initiation factor 3 RNA-binding subunit antibody; Eukaryotic translation initiation factor 3 subunit 4 antibody; Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody; Eukaryotic translation initiation factor 3 subunit G antibody; Eukaryotic translation initiation factor 3 subunit p42 antibody; Eukaryotic translation initiation factor 3; subunit 4; formerly antibody; Subunit 4 (delta 44kD) antibody; Subunit 4 delta antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Eukaryotic translation initiation factor 3 subunit G protein (2-320AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，EIF3G Antibody (CSB-PA03099A0Rb)，的标记方式是Non-conjugated。对于EIF3G Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA03099B0Rb EIF3G Antibody, HRP conjugated ELISA
    FITC CSB-PA03099C0Rb EIF3G Antibody, FITC conjugated
    Biotin CSB-PA03099D0Rb EIF3G Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA.; (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.
  • 基因功能参考文献：
    1. The disease-associated allele increases EIF3G mRNA expression. EIF3G is located in the narcolepsy risk locus and EIF3G expression correlates with PPAN and P2RY11 expression. PMID: 25669430
    2. Important roles for eIF3g in the translation initiation machinery and in DNA degradation during apoptosis. PMID: 24080033
    3. down-regulation of eIF3g inhibits the efficiency of nonsense-mediated mRNA decay, which is tightly coupled to CT but not to ET PMID: 22493286
    4. Furthermore, we found that overexpression of DISC1 in SH-SY5Y cells induces the assembly of eIF3- and TIA-1-positive stress granules (SGs), discrete cytoplasmic granules formed in response to environmental stresses. PMID: 16243297
    5. AIF overexpression specifically resulted in the activation of caspase-7, thereby amplifying the inhibition of protein PMID: 17094969

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Colocalizes with AIFM1 in the nucleus and perinuclear region.
  • 蛋白家族：
    EIF-3 subunit G family
  • 数据库链接：

    HGNC: 3274

    OMIM: 603913

    KEGG: hsa:8666

    STRING: 9606.ENSP00000253108

    UniGene: Hs.529059