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EDEM1 Antibody

  • 货号：
    CSB-PA838793LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA838793LA01HU diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) EDEM1 Polyclonal antibody
  • Uniprot No.：
    Q92611
  • 基因名：
  • 别名：
    EDEM1 antibody; EDEM antibody; KIAA0212ER degradation-enhancing alpha-mannosidase-like protein 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human ER degradation-enhancing alpha-mannosidase-like protein 1 protein (46-128AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，EDEM1 Antibody (CSB-PA838793LA01HU)，的标记方式是Non-conjugated。对于EDEM1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA838793LB01HU EDEM1 Antibody, HRP conjugated ELISA
    FITC CSB-PA838793LC01HU EDEM1 Antibody, FITC conjugated
    Biotin CSB-PA838793LD01HU EDEM1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.
  • 基因功能参考文献：
    1. EDEM1 colocalizes with the selective autophagy cargo receptors p62/SQSTM1, neighbor of BRCA1 gene 1 (NBR1) and autophagy-linked FYVE (Alfy) protein, and becomes engulfed by autophagic isolation membranes. PMID: 24664425
    2. Endoplasmic reticulum degradation-enhancing alpha-mannosidase-like protein 1 targets misfolded HLA-B27 dimers for endoplasmic reticulum-associated degradation. PMID: 25132672
    3. miR-581 promotes hepatitis B virus surface antigen expression by targeting Dicer and EDEM1 in HepG2 cells. PMID: 24913918
    4. EDEM1 associates with lifespan in animal models, although not humans. PMID: 24037343
    5. analysis of a shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins PMID: 23233672
    6. Tyrosinase degradation is prevented when EDEM1 lacks the intrinsically disordered region PMID: 22905195
    7. we suggest that a nascent glycoprotein can normally dissociate from EDEM1 and be rescued from endoplasmic reticulum associated degradation by reentering calnexin-refolding cycles PMID: 21917589
    8. signal sequence cleavage functionally regulated the association of EDEM1-soluble and membrane-integrated isoforms with distinct ERAD machinery and substrates. PMID: 21632540
    9. EDEM1 protein recognition may be determined by the structure of the endoplasmic reticulum-associated protein degradation substrate in a study of mutant ricin A-chain transport to the cytosol. PMID: 21388347
    10. mannose trimming enables delivery of a substrate glycoprotein from EDEM1 to late endoplasmic reticulum degredation steps through association with XTP3-B PMID: 21062743
    11. The EDEM1 activity trims mannose from the C branch of N-glycans in vivo. PMID: 20065073
    12. EDEM1 promotes correct folding and enhanced degradation of rod opsin. PMID: 19934218
    13. EDEM was shown to extract misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle [EDEM] PMID: 12610306
    14. EDEM1 is a soluble protein of the ER lumen in HEK293 cells PMID: 15579471
    15. These results indicate that EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation so that unstable misfolded proteins can be accommodated by the dislocon for ERAD. PMID: 16629899
    16. the endoplasmic reticulum quality control vesicular transport pathway using EDEM1 does not involve the COPII exit sites PMID: 17360537
    17. Cells with defective EDEM1 turnover contain unphysiologically high levels of EDEM1, show enhanced ER associated degradation activity and are characterized by impaired capacity to efficiently complete maturation of model glycopolypeptides. PMID: 18452703
    18. endogenous EDEM1 in cells not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy PMID: 19266160
    19. EDEM1 specifically binds nonnative proteins in a glycan-independent manner. PMID: 19524542

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Glycosyl hydrolase 47 family
  • 数据库链接：

    HGNC: 18967

    OMIM: 607673

    KEGG: hsa:9695

    STRING: 9606.ENSP00000256497

    UniGene: Hs.224616