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E2F5 Antibody

  • 货号：
    CSB-PA624021LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, PC-3 whole cell lysate, Rat liver tissue, Mouse liver tissue
      All lanes: E2F5 antibody at 5µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 38, 21 kDa
      Observed band size: 38 kDa
    • IHC image of CSB-PA624021LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) E2F5 Polyclonal antibody
  • Uniprot No.：
    Q15329
  • 基因名：
  • 别名：
    E2F 5 antibody; E2F transcription factor 5 antibody; E2F transcription factor 5 p130 binding antibody; E2F-5 antibody; E2f5 antibody; E2F5_HUMAN antibody; p130 binding antibody; Transcription factor E2F5 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human Transcription factor E2F5 protein (193-298AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，E2F5 Antibody (CSB-PA624021LA01HU)，的标记方式是Non-conjugated。对于E2F5 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA624021LB01HU E2F5 Antibody, HRP conjugated ELISA
    FITC CSB-PA624021LC01HU E2F5 Antibody, FITC conjugated
    Biotin CSB-PA624021LD01HU E2F5 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
  • 基因功能参考文献：
    1. High E2F5 expression is associated with Glioma. PMID: 29362021
    2. knockdown of E2F5 and PFTK1 mimicked the tumor-suppressive effects of miR-1-3p overexpression on PCa progression. Conversely, concomitant knockdown of miR-1-3p and E2F5 and PFTK1 substantially reversed the inhibitory effects of either E2F5 or PFTK1 silencing alone. PMID: 30185212
    3. miR-1179 overexpression could also inhibit tumor growth in vivo by suppressing the expression of E2F5. PMID: 29859832
    4. FOXN3 functions as a tumor suppressor in hepatocellular carcinoma by downregulating the expression of E2F5. PMID: 27259277
    5. Taken together, these findings indicated that SNHG16 induces breast cancer cell migration by competitively binding miR-98 with E2F5. PMID: 28232182
    6. Our data collectively indicate that miR-613 functions as a tumor suppressor in retinoblastoma through downregulating E2F5, supporting the targeting of the novel miR-613/E2F5 axis as a potentially effective therapeutic approach for retinoblastoma. PMID: 28351331
    7. MiR-34a was down-regulated in colorectal cancer cells and inversely correlated with FMNL2 and E2F5 expressions. Our study suggests that miR-34a is an important tumor suppressor of CRC progression by targeting FMNL2 and E2F5. PMID: 26103003
    8. miR-98 regulates muscle differentiation by altering the expression of the transcription factor E2F5 and, in turn, of multiple E2F5 targets. PMID: 25422988
    9. In hepatocellular carcinoma cells hepatitis B virus down-regulates E2F5 expression by up-regulating expression of miR-181a. PMID: 24529171
    10. expression of E2F5 in esphageal squamous cell cancer (ESCC) may be correlated with a worse prognosis of patients with ESCC PMID: 24324077
    11. E2F5 may be a novel potential candidate marker for malignant prostate cancer PMID: 23377984
    12. Data indicate that E2F5 is commonly overexpressed in primary hepatocellular carcinomas and show that E2F5 knockdown significantly repressed the growth of HCC cells. PMID: 21274376
    13. cell-cycle regulatory protein E2F5 might play a significant role in epithelial ovarian cancer pathogenesis PMID: 20181230
    14. Diverting the function of E2F5 from a cell-cycle repressor into an activator might contribute to the higher oncogenic potential of HPV18 when compared with other high-risk HPV types. PMID: 20639900
    15. the subcellular distribution of E2F-5 is tightly regulated in intact cells, through multiple functional domains that direct nucleocytoplasmic shuttling of this protein. PMID: 12089160
    16. EBV LPM1 blocks p16INK4 pathway by promoting nuclear export of E2F-5. PMID: 12860972
    17. E2F5 protein is down-regulated late in embryogenesis PMID: 16172982
    18. Overexpression of E2F5/p130 in post-mitotic lens fiber cells does not affect normal differentiation, but can inhibit inappropriate cell cycle reentry induced by activator E2Fs. P130 is key player in inhibitory process. PMID: 18385796
    19. Overexpression of E2F-5 correlates with a pathological basal phenotype and a worse clinical outcome. PMID: 19259095

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    E2F/DP family
  • 数据库链接：

    HGNC: 3119

    OMIM: 600967

    KEGG: hsa:1875

    STRING: 9606.ENSP00000398124

    UniGene: Hs.445758