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DNAJA3 Antibody

  • 货号：
    CSB-PA836207LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, A549 whole cell lysate
      All lanes: DNAJA3 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 53, 50, 34 kDa
      Observed band size: 53 kDa
    • IHC image of CSB-PA836207LA01HU diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA836207LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of MCF-7 cells with CSB-PA836207LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating DNAJA3 in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA836207LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
      Lane 2: CSB-PA836207LA01HU (5µg) + Hela whole cell lysate (0.5mg)
      Lane 3: Hela whole cell lysate (20µg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) DNAJA3 Polyclonal antibody
  • Uniprot No.：
    Q96EY1
  • 基因名：
    DNAJA3
  • 别名：
    DNAJA3 antibody; HCA57 antibody; TID1DnaJ homolog subfamily A member 3 antibody; mitochondrial antibody; DnaJ protein Tid-1 antibody; hTid-1 antibody; Hepatocellular carcinoma-associated antigen 57 antibody; Tumorous imaginal discs protein Tid56 homolog antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human DnaJ homolog subfamily A member 3, mitochondrial protein (86-184AA )
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，DNAJA3 Antibody (CSB-PA836207LA01HU)，的标记方式是Non-conjugated。对于DNAJA3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA836207LB01HU DNAJA3 Antibody, HRP conjugated ELISA
    FITC CSB-PA836207LC01HU DNAJA3 Antibody, FITC conjugated
    Biotin CSB-PA836207LD01HU DNAJA3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.
  • 基因功能参考文献：
    1. Impaired stoichiometry between mtHsp40 and mtHsp70 promotes Opa1L cleavage, leading to cristae opening, decreased OXPHOS, and triggering of mitochondrial fragmentation after reduction in their chaperone function. PMID: 25904328
    2. The Role of the Phylogenetically Conserved Cochaperone Protein Droj2/DNAJA3 in NF-kappaB Signaling. PMID: 26245905
    3. TID1 prevents complex I aggregation and support the existence of a TID1-mediated stress response to ATP synthase inhibition PMID: 24492964
    4. Tid1 acts as a tumor suppressor by inhibiting EGFR signaling through interaction with EGFR/HSP70/HSP90 and enhancing EGFR ubiquitinylation and degradation. PMID: 23698466
    5. Data show that elevated DnaJA3 induces dynamin-related protein 1 (Drp1)-depedendent mitochondrial fragmentation and decreased cell viability. PMID: 22595283
    6. loss of hTid-1(S) expression in the basal layer of skin epidermis correlates with the enhanced HSP27 phosphorylation, keratinocyte hyperproliferation, and excess actin cytoskeleton organization in lesional psoriatic skin PMID: 22692211
    7. Tid1 and CHIP play pivotal roles in affecting the levels of ErbB2 protein, and that both are significant prognostic indicators of breast cancer patient survival. PMID: 21710689
    8. findings denote hTid-1(S) as an essential regulatory component of MetR signaling PMID: 21242965
    9. hTid1 negatively regulates the expression and transcriptional activity of STAT5b and suppresses the growth of hematopoietic cells transformed by an oncogenic form of STAT5b. PMID: 21106534
    10. htid is a tissue independent and evolutionarily conserved suppressor of ErbB-2. PMID: 20565727
    11. amino-terminal segment of APC promotes cell sensitivity to apoptosis modulated through its binding to 40- and 43-kilodalton hTID-1 isoforms. PMID: 19900451
    12. role as a repressor of Ikappa B kinase beta subunit PMID: 11927590
    13. Tid-1S in Th2 cells following activation has a role in induction of apoptosis resistance during the activation of Th2 cells PMID: 12879007
    14. an important cell death regulator and could exert tumor suppressor activity PMID: 15156195
    15. findings suggest that the status of hTid-1 in gliomas may contribute to their susceptibility to cell death triggers PMID: 15589840
    16. hTid-1 represses the activity of NF-kappaB through physical and functional interactions with the IKK complex and IkappaB PMID: 15601829
    17. the carboxyl-terminal end of TID1 (residues 224-429) bound to Trk at the activation loop (Tyr(P)(683)-Tyr(684)(P)(684) PMID: 15753086
    18. Tid1 is critical for the transition of double-negative to double-positive cells in early T cell development through modulation of antiapoptotic bcl-2 expression. PMID: 15879105
    19. Tid1 negatively regulates the motility and metastasis of breast cancer cells, most likely through attenuation of nuclear factor kappaB activity on the promoter of the IL8 gene. PMID: 16204048
    20. hTid1 may act as a chaperone to facilitate the folding, processing and maturation of Epstein-Barr virus-encoded BARF1 protein. PMID: 16518412
    21. The association of Tid1 with chaperones and/or protein substrates in the cytosol provides a mechanism for the alternate fates and functions of Tid1 in mitochondrial and nonmitochondrial pathways. PMID: 16531398
    22. The association of the endogenous Tid50/Tid48 proteins with the adenomatous polyposis coli (APC) tumor suppressor is shown. PMID: 17588722
    23. The expression of the genes htid-1 and APC was altered in colorectal tumors. PMID: 18097612

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  • 亚细胞定位：
    Mitochondrion matrix. Cytoplasm, cytosol. Cell junction, synapse, postsynaptic cell membrane; Peripheral membrane protein.
  • 组织特异性：
    Widely expressed with highest levels in heart, liver, lung and skeletal muscles. Also expressed in keratinocytes.
  • 数据库链接：

    HGNC: 11808

    OMIM: 608382

    KEGG: hsa:9093

    STRING: 9606.ENSP00000262375

    UniGene: Hs.459779