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DMAP1 Antibody

  • 货号：
    CSB-PA873611LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, Colo320 whole cell lysate
      All lanes: DMAP1 antibody at 11µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 53 kDa
      Observed band size: 53 kDa
    • IHC image of CSB-PA873611LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA873611LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) DMAP1 Polyclonal antibody
  • Uniprot No.：
    Q9NPF5
  • 基因名：
    DMAP1
  • 别名：
    DMAP1 antibody; KIAA1425DNA methyltransferase 1-associated protein 1 antibody; DNMAP1 antibody; DNMT1-associated protein 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human DNA methyltransferase 1-associated protein 1 protein (250-300AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，DMAP1 Antibody (CSB-PA873611LA01HU)，的标记方式是Non-conjugated。对于DMAP1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA873611LB01HU DMAP1 Antibody, HRP conjugated ELISA
    FITC CSB-PA873611LC01HU DMAP1 Antibody, FITC conjugated
    Biotin CSB-PA873611LD01HU DMAP1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.
  • 基因功能参考文献：
    1. A novel tumour suppressor gene, MDGA2, which is frequently inactivated by promoter methylation in Gastric cancer, exerts a tumour suppressive function by cooperating with DMAP1 to activate the p53/p21 signalling cascade. PMID: 26206665
    2. Targeted knockdown of human DNA methyltransferase 1 expression restores the expression levels of tumor suppressor genes, thus inhibiting the proliferation of cholangiocarcinoma cell line cells. PMID: 24361215
    3. These results suggest that DMAP1 is a critical regulator of ATM activity and function PMID: 23318425
    4. positive staining for DNMT1 in clinical cancer specimens was significantly linked to lower rates of response to treatments and shorter survival of patients with pharyngeal cancer. PMID: 21284050
    5. Knocking down DMAP1 caused hypomethylation of the DNA repair products. PMID: 20864525
    6. MMTR is an intrinsic negative cell cycle regulator that modulates the CAK kinase activity via interaction with MAT1. PMID: 20920467
    7. RGS6 inhibited the transcriptional repressor activity of DMAP1. PMID: 14734556

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Targeted to replication foci throughout S phase by DNMT1.
  • 数据库链接：

    HGNC: 18291

    OMIM: 605077

    KEGG: hsa:55929

    STRING: 9606.ENSP00000312697

    UniGene: Hs.8008