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DGKE Antibody

  • 货号：
    CSB-PA070073
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    P52429
  • 基因名：
  • 别名：
    DGKE; DAGK5; Diacylglycerol kinase epsilon; DAG kinase epsilon; Diglyceride kinase epsilon; DGK-epsilon
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human DGK-ε.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently.
  • 基因功能参考文献：
    1. mutations can lead to atypical hemolytic uremic syndrome or membranoproliferative glomerulonephritis PMID: 28526779
    2. Letter/Case Report: atypical haemolytic uraemic syndrome in a Japanese patient with DGKE genetic mutations. PMID: 26018111
    3. Report DGKE intronic mutations located beyond the exon-intron boundaries in familial hemolytic uremic syndrome. PMID: 25854283
    4. Data suggest that complement dysregulation influences the onset and disease severity in carriers of diacylglycerol kinase-epsilon mutations PMID: 25135762
    5. DGKE silencing in resting endothelial cells does not affect complement activation at their surface. PMID: 25498910
    6. Our study expands the clinical phenotypes associated with mutations in DGKE and challenges the benefits of complement blockade treatment in such patients PMID: 24511134
    7. DGKzeta localizes to the nucleus and is considered to regulate nuclear diacylglycerol signaling.[review] PMID: 24119575
    8. Fully activating high-density transfected muscarinic receptors (M1Rs) by oxotremorine-M (Oxo-M) leads to similar calcium, DAG, and PKC signals, but PIP2 is depleted. PMID: 23630338
    9. Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome. PMID: 23542698
    10. Substrate specificity of DGKE is not a consequence of competition with hydrolysis of ATP. PMID: 23261795
    11. performed homozygosity mapping and whole exome sequencing in a Turkish consanguineous family and identified DGKE gene variants as the cause of a membranoproliferative-like glomerular microangiopathy PMID: 23274426
    12. Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity. PMID: 22511757
    13. A role for diacylglycerol kinase (DGK) and its downstream product phosphatidic acid (PA) in ANCA-induced neutrophil exocytosis, is reported. PMID: 21833457
    14. The region responsible for this arachidonoyl specificity is the lipoxygenase (LOX)-like motif found in the accessory domain, adjacent to DGKvarepsilon's catalytic site. PMID: 22266092
    15. diacylglycerol kinase-epsilon (DGKepsilon) has less preference for the acyl chain at the sn-1 position of diacylglycerol (DAG) than the one at the sn-2 position PMID: 22108654
    16. DGK activity is reduced by oxidative stress in human mesangial cells cultured under high glucose conditions. PMID: 21725595
    17. The hydrophobic domain of diacylglycerol kinase epsilon does not contribute to substrate specificity but plays a role in permanently sequestering the enzyme to a membrane. PMID: 17455907
    18. The alpha and zeta isoforms of diacylglycerol kinase are inhibited by 2,3-dioleoylglycerol, but not the more substrate-selective epsilon isoform PMID: 18004883
    19. Substrate specificity of diacylglycerol kinase epsilon is determined by selectivity of the sn-1 and sn-2 acyl chains phosphatidic acid or diacylglycerol. PMID: 19744926

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  • 相关疾病：
    Nephrotic syndrome 7 (NPHS7); Hemolytic uremic syndrome atypical 7 (AHUS7)
  • 亚细胞定位：
    Membrane; Single-pass membrane protein. Cytoplasm.
  • 蛋白家族：
    Eukaryotic diacylglycerol kinase family
  • 组织特异性：
    Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
  • 数据库链接：

    HGNC: 2852

    OMIM: 601440

    KEGG: hsa:8526

    STRING: 9606.ENSP00000284061

    UniGene: Hs.239514