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DDX11 Antibody

  • 货号：
    CSB-PA960020
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA960020(DDX11 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA960020(DDX11 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q96FC9
  • 基因名：
    DDX11
  • 别名：
    CHL1 antibody; CHL1 related helicase gene 1 antibody; CHL1-like helicase homolog antibody; CHL1-related protein 1 antibody; CHLR1 antibody; Ddx11 antibody; DDX11_HUMAN antibody; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 11 antibody; DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 antibody; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog; S. cerevisiae) antibody; DEAD/H box antibody; DEAD/H box protein 11 antibody; hCHLR1 antibody; Keratinocyte growth factor regulated gene 2 protein antibody; Keratinocyte growth factor-regulated gene 2 protein antibody; KRG 2 antibody; KRG-2 antibody; KRG2 antibody; MGC133249 antibody; MGC9335 antibody; OTTHUMP00000238778 antibody; OTTHUMP00000238780 antibody; OTTHUMP00000238784 antibody; OTTHUMP00000238785 antibody; Probable ATP dependent RNA helicase DDX11 antibody; Probable ATP-dependent RNA helicase DDX11 antibody; WABS antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Fusion protein of Human DDX11
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    DNA-dependent ATPase and ATP-dependent DNA helicase that participates in various functions in genomic stability, including DNA replication, DNA repair and heterochromatin organization as well as in ribosomal RNA synthesis. Its double-stranded DNA helicase activity requires either a minimal 5'-single-stranded tail length of approximately 15 nt (flap substrates) or 10 nt length single-stranded gapped DNA substrates of a partial duplex DNA structure for helicase loading and translocation along DNA in a 5' to 3' direction. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended up to 500 bp by the replication protein A (RPA) or the cohesion CTF18-replication factor C (Ctf18-RFC) complex activities. Shows also ATPase- and helicase activities on substrates that mimic key DNA intermediates of replication, repair and homologous recombination reactions, including forked duplex, anti-parallel G-quadruplex and three-stranded D-loop DNA molecules. Plays a role in DNA double-strand break (DSB) repair at the DNA replication fork during DNA replication recovery from DNA damage. Recruited with TIMELESS factor upon DNA-replication stress response at DNA replication fork to preserve replication fork progression, and hence ensure DNA replication fidelity. Cooperates also with TIMELESS factor during DNA replication to regulate proper sister chromatid cohesion and mitotic chromosome segregation. Stimulates 5'-single-stranded DNA flap endonuclease activity of FEN1 in an ATP- and helicase-independent manner; and hence it may contribute in Okazaki fragment processing at DNA replication fork during lagging strand DNA synthesis. Its ability to function at DNA replication fork is modulated by its binding to long non-coding RNA (lncRNA) cohesion regulator non-coding RNA DDX11-AS1/CONCR, which is able to increase both DDX11 ATPase activity and binding to DNA replicating regions. Plays also a role in heterochromatin organization. Involved in rRNA transcription activation through binding to active hypomethylated rDNA gene loci by recruiting UBTF and the RNA polymerase Pol I transcriptional machinery. Plays a role in embryonic development and prevention of aneuploidy. Involved in melanoma cell proliferation and survival. Associates with chromatin at DNA replication fork regions. Binds to single- and double-stranded DNAs.; (Microbial infection) Required for bovine papillomavirus type 1 regulatory protein E2 loading onto mitotic chromosomes during DNA replication for the viral genome to be maintained and segregated.
  • 基因功能参考文献：
    1. DDX11 orchestrates jointly with 9-1-1 and its loader, RAD17, DNA damage tolerance at sites of bulky lesions, and endogenous abasic sites. These functions may explain the essential roles of DDX11 and its similarity with 9-1-1 during development. PMID: 30061412
    2. We present two new cases of Warsaw Breakage Syndrome (WABS), an autosomal recessive cohesinopathy, in sisters aged 13 and 11 years who both had compound heterozygous mutations in DDX11 PMID: 28960803
    3. A mutation has been identified in HPV16 E2 that abrogates interaction with ChlR1, and it was shown that ChlR1 regulates the chromatin association of HPV16 E2 and that this virus-host interaction is essential for viral episome maintenance. PMID: 27795438
    4. In this study, we found that cohesion establishment factors, like CHlR1, cooperatively stimulate endonuclease activity of hFen1 in in vivo mimic condition, including replication protein-A-coated DNA and high salt. PMID: 26032365
    5. DDX11 and Tim proteins physically and functionally interact and act in concert to preserve replication fork progression in perturbed conditions. PMID: 26503245
    6. Q Motif Is Involved in DNA Binding but Not ATP Binding in ChlR1 Helicase PMID: 26474416
    7. our results indicate that DDX11 functions as a positive regulator of rRNA transcription and provides a novel insight into the pathogenesis of WABS. PMID: 26089203
    8. A distinct triplex DNA unwinding activity of ChlR1 helicase. PMID: 25561740
    9. ChlR1 plays a critically important role in cellular replication and/or DNA repair. [review] PMID: 24487782
    10. Data indicate a homozygous mutation (c.788G>A [p.R263Q]) in DDX11 in three affected siblings with severe intellectual disability and many of the congenital abnormalities reported in the Warsaw breakage syndrome (WABS) original case. PMID: 23033317
    11. helicase DDX11 is expressed at high levels in primary and metastatic melanoma, and that interfering with its expression leads to severe chromosome segregation defects, telomere shortening, and massive melanoma cell apoptosis. PMID: 23116066
    12. The results identify novel roles of Ddx11 during embryo morphogenesis and demonstrate that the activity of its motif V is essential for DDX11 function. PMID: 22678773
    13. Wild-type ChlR1 required a minimal 5' single-stranded DNA tail of 15 nucleotides to efficiently unwind a simple duplex DNA substrate. PMID: 22102414
    14. The ChlR1 is involved in the proper formation of heterochromatin, which in turn contributes to global nuclear organization and pleiotropic effects. PMID: 21854770
    15. These data provide evidence that the association of bovine papillomavirus E2 with human ChlR1 contributes to a loading mechanism during DNA replication rather than direct tethering during mitotic division. PMID: 21489590
    16. Timeless associates with the cohesion-promoting DNA helicase ChlR1, which, when overexpressed, partially alleviates the cohesion defect of cells depleted of Timeless-Tipin. PMID: 20124417
    17. We present preliminary analysis of a strong candidate gene in the region, the DNA helicase DDX11. In conclusion, we report mapping of the first locus that determines mean telomere length in humans. PMID: 15520935
    18. human ChlR1 is required for sister chromatid cohesion and, hence, normal mitotic progression. PMID: 17105772
    19. These studies provide compelling evidence that ChlR1 association is required for loading the papillomavirus E2 protein onto mitotic chromosomes and represents a kinetochore-independent mechanism for viral genome maintenance and segregation. PMID: 17189189
    20. hChlR1 has a role in the establishment of sister chromatid cohesion, with Ctf18-RFC and Fen1 PMID: 18499658

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  • 相关疾病：
    Warsaw breakage syndrome (WBRS)
  • 亚细胞定位：
    Nucleus. Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle pole. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.; Chromosome.
  • 蛋白家族：
    DEAD box helicase family, DEAH subfamily, DDX11/CHL1 sub-subfamily
  • 组织特异性：
    Expressed in melanoma cells. Not detected in epidermal melanocytes of normal skin (at protein level). Highly expressed in spleen, B-cells, thymus, testis, ovary, small intestine and pancreas. Very low expression seen in brain. Expressed in dividing cells
  • 数据库链接：

    HGNC: 2736

    OMIM: 601150

    KEGG: hsa:1663

    STRING: 9606.ENSP00000384703

    UniGene: Hs.443960