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DAPK3 (Ab-265) Antibody

  • 货号：
    CSB-PA994602
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from HuvEc cells, using DAPK3 (Ab-265) antibody.
    • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using DAPK3 (Ab-265) antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) DAPK3 Polyclonal antibody
  • Uniprot No.：
    O43293
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized non-phosphopeptide derived from Human DAPK3 around the phosphorylation site of threonine 265 (R-M-T(p)-I-A).
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.
  • 基因功能参考文献：
    1. For the first time, the present study showed that anacardic acid induces cell apoptosis of prostatic cancer through autophagy by ER stress/DAPK3/Akt signaling pathway. PMID: 28731173
    2. Activation of AKT negatively regulates the pro-apoptotic function of DAPK3 in prostate cancer. PMID: 27126362
    3. Methylation of DAPK3 at CpG1 but not CpG2 was lower in men with type 2 diabetes compared with healthy control men. Moreover, a glucose challenge was associated with lower CpG1 and CpG2 methylation in DAPK3 in both men with type 2 diabetes and healthy men. PMID: 28011458
    4. DAPK3 controls proliferation, migration and tumor growth through activation of ERK/c-Myc signaling in lung adenocarcinoma cells. PMID: 28075459
    5. The results revealed mutations caused a significant structural variation in DAPK3, concentrated in flexible loops forming part of the ATP binding pocket which have been associated with very low kinase activity, and the cellular progression towards cancer PMID: 26748242
    6. Cellular and mouse modeling studies show that DAPK3 is a tumor suppressor gene and is important in early development. Also, its expression is downregulated in more aggressive breast cancer relative to less aggressive and normal patient samples. PMID: 25304685
    7. ROCK1 and ZIPK have diverse, but predominantly distinct regulatory functions in vascular SMC and that ROCK1-mediated activation of ZIPK is not involved in most of these functions. PMID: 25723491
    8. DAPK3 plays a central role in preventing miR-17/miR-20a depletion-induced genome instability and in miR-17/miR-20a overexpression-triggered tumor formation. PMID: 26117336
    9. These findings indicated that ZIPK may also be involved in the regulation of the cell cycle in human cells, by interacting with HsCdc14A. PMID: 25503649
    10. findings suggest that ZIPK plays a role in the progression and completion of cytokinesis through MRLC phosphorylation PMID: 25769953
    11. Depletion of ZIPK resulted in impairment of cultured VSMC migration and contraction. PMID: 24633547
    12. ZIPK has a crucial role in regulation of ubiquitination and degradation of the AR PMID: 23146908
    13. Interaction of RhoD and ZIP kinase modulates actin filament assembly and focal adhesion dynamics. PMID: 23454120
    14. ZIPK may serve as a transcriptional regulator of canonical Wnt/beta-catenin signaling through interaction with NLK/TCF4. PMID: 21454679
    15. Results suggest that DAPK3 is a tumor suppressor in which loss-of-function mutations promote increased cell survival, proliferation, cellular aggregation. PMID: 21487036
    16. The NLS2 of human ZIPK functions the nucleus-directing motif, but only upon dephosphorylation of the adjacent T299 residue. PMID: 20854903
    17. Dlk plays a positive role in modulating death-related signaling pathways. PMID: 20085750
    18. These results suggest that ZIPK, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis PMID: 12917339
    19. data identify hZIPK as the unique SMPP-1-associated kinase expressed in human vesicular smooth muscle and support a role for Rho in promoting the hZIPK-MBS interaction PMID: 15292222
    20. The specific expression pattern of TCP10L and interaction with DAPK-3 implies that TCP10L might play crucially important roles in spermatogenesis through its interaction with DAPK-3. PMID: 15910542
    21. ZIP kinase plays a crucial role in androgen receptor-mediated transcription. PMID: 18084323
    22. These results suggest that UbcH5 regulates ZIPK accumulation in PML-NBs by interacting with ZIPK and stimulating its ubiquitination. PMID: 18515077
    23. ZIPK is a tumor suppresser gene and plays an important role in gastric carcinoma PMID: 19117059

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  • 亚细胞定位：
    Nucleus. Cytoplasm.; [Isoform 1]: Nucleus. Cytoplasm.; [Isoform 2]: Nucleus. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, DAP kinase subfamily
  • 组织特异性：
    Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.
  • 数据库链接：

    HGNC: 2676

    OMIM: 603289

    KEGG: hsa:1613

    STRING: 9606.ENSP00000301264

    UniGene: Hs.631844