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  4. DAB2 Antibody

DAB2 Antibody

  • 货号：
    CSB-PA337435
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA337435(DAB2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA337435(DAB2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
    • Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Hela cells, Primary antibody: CSB-PA337435(DAB2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
  • 其他：

产品详情

  • Uniprot No.：
    P98082
  • 基因名：
  • 别名：
    DAB2 antibody; DOC2 antibody; Disabled homolog 2 antibody; Adaptor molecule disabled-2 antibody; Differentially expressed in ovarian carcinoma 2 antibody; DOC-2 antibody; Differentially-expressed protein 2 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Fusion protein of Human DAB2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:500-1:2000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containing non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR. Involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development. Required for recycling of the TGF-beta receptor. Involved in CFTR trafficking to the late endosome. Involved in several receptor-mediated signaling pathways. Involved in TGF-beta receptor signaling and facilitates phosphorylation of the signal transducer SMAD2. Mediates TFG-beta-stimulated JNK activation. May inhibit the canoniocal Wnt/beta-catenin signaling pathway by stabilizing the beta-catenin destruction complex through a competing association with axin preventing its dephosphorylation through protein phosphatase 1 (PP1). Sequesters LRP6 towards clathrin-mediated endocytosis, leading to inhibition of Wnt/beta-catenin signaling. May activate non-canonical Wnt signaling. In cell surface growth factor/Ras signaling pathways proposed to inhibit ERK activation by interrupting the binding of GRB2 to SOS1 and to inhibit SRC by preventing its activating phosphorylation at 'Tyr-419'. Proposed to be involved in modulation of androgen receptor (AR) signaling mediated by SRC activation; seems to compete with AR for interaction with SRC. Plays a role in the CSF-1 signal transduction pathway. Plays a role in cellular differentiation. Involved in cell positioning and formation of visceral endoderm (VE) during embryogenesis and proposed to be required in the VE to respond to Nodal signaling coming from the epiblast. Required for the epithelial to mesenchymal transition, a process necessary for proper embryonic development. May be involved in myeloid cell differentiation and can induce macrophage adhesion and spreading. May act as a tumor suppressor.
  • 基因功能参考文献：
    1. Estrogen-induced miR-191 was identified as a direct upstream regulator of DAB2 in ER-positive breast cancer cells. PMID: 29247596
    2. DAB2 is a frequent target of epigenetic silencing in oral carcinomas and may be potentially used for tumor detection PMID: 29254794
    3. Dab2 Ser723 phosphorylation is a key molecular event in thrombin-stimulated inside-out signaling and platelet activation, contributing to a new function of Dab2 in thrombin signaling. PMID: 28876503
    4. DAB2 might be involved in excessive aldosterone biosynthesis and correlate with specific clinical characteristics of aldosterone-producing adenoma patients. PMID: 28514805
    5. the results indicated that substrate stiffness could regulate EMT of cervical cancer cell lines HeLa and SiHa at least partially through miR-106b and its downstream target DAB2. PMID: 28498390
    6. DAB2 can suppress the ERK signaling, but correlate to have TGF-beta-induced epithelial-to-mesenchymal transition (EMT) in esophageal squamous cell carcinomas (ESCCs). PMID: 27036032
    7. The various novel mechanisms are described by which Dab2 mediates an array of signaling events with vast physiological consequences. PMID: 27417122
    8. In conclusion, DAB2 and Intelectin-1 are newly identified positive markers of mesothelioma and have potential to be included in future immunohistochemical marker panels for differentiation of epithelioid mesothelioma from pulmonary adenocarcinoma PMID: 28394802
    9. abundances of megalin and Dab2 (p = 0.046) were reduced in infected placentas from women with LBW deliveries PMID: 27072056
    10. Data show that miR106b was frequently up-regulated in human cervical carcinoma tumors and cell lines and inversely correlated with DAB2 expression. Its regulation in under TGF-beta1 which contributes to cell migration by targeting DAB2 in cervical carcinoma. PMID: 26769181
    11. these findings reveal that DAB2 is critical for controlling inflammatory signaling during phenotypic polarization of macrophages PMID: 26927671
    12. DAB2 regulated the cell migration associated genes in PC3 cells, and the differential DAB2 expression between LNCaP and PC3 cells was partly regulated by histone 4 acetylation. PMID: 26143155
    13. Dab2 depletion also increases the rescued protein half-life of DeltaF508 CFTR by ~18% and ~91%, respectively PMID: 25879443
    14. These results indicated that miR-93 plays an important role in the initiation and progression of NPC by targeting Dab2 and the miR-93/Dab2 pathway may contribute to the development of novel therapeutic strategies for NPC in the future. PMID: 25892549
    15. DAB2 expression is decreased in Non-Small Cell Lung Cancer , and the frequent methylation event at sites -86 to 226 of the DAB2 gene could contribute to the downregulation of DAB2. PMID: 25719979
    16. Attenuating Dab2 expression in K562 cells diminished TfR internalization and increased surface levels of TfR concomitantly with a decrease in Tf uptake and erythroid differentiation. PMID: 24889971
    17. Results suggest that endogenous Dab2 exacerbates central nervous system inflammation, potentially acting to up-regulate reactive oxygen species expression in macrophages and microglia, and that it is of potential pathogenic relevance in multiple sclerosis PMID: 24252604
    18. Numb specifically regulates NPC1L1-mediated cholesterol absorption both in human intestine and liver, distinct from ARH and Dab2, which selectively participate in LDLR-mediated LDL uptake. PMID: 25331956
    19. Akt1 and Akt2 are involved in albumin endocytosis, and phosphorylation of Dab2 by Akt induces albumin endocytosis in proximal tubule epithelial cells. PMID: 25253241
    20. The data support a model in which Dab2 regulates the domain localization of TbetaRI in the membrane, balancing TGF-beta signaling via the Smad and JNK pathways. PMID: 24648493
    21. breast cancer cell was deficient in Dab2 expression and related receptor endocytosis-mediated TGF-beta depletion, which may contribute to the accumulation of TGF-beta in tumor microenvironment and the induction of immune tolerance. PMID: 24638085
    22. miR-145 regulates the migration and invasion of highly invasive prostate cancer cells through targeting DAB2 gene PMID: 24846918
    23. Aberrant hypermethylation and reduced expression of disabled-2 promote the development of lung cancers. PMID: 24002585
    24. Reduced Dab2 or loss of Dab2 expression significantly correlates with lower classification of meningiomas and negatively correlates with the invasive ability of adjacent tissues. PMID: 24608456
    25. Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266
    26. Megalin and Dab2 were expressed in prostate and colon epithelial cells, which was markedly enhanced following treatment with retinoic acid PMID: 23909735
    27. These data suggest that Dab2-mediated recruitment of EH domain proteins selectively drives the internalization of the Dab2 cargo, integrin b1. PMID: 22648170
    28. Data indicate that disabled-2 (Dab2) sulfatide-binding motif contains two helices when embedded in micelles, reversibly binds to sulfatides with moderate affinity, lies parallel to the micelle surface. PMID: 22977233
    29. The Dab2 gene is inactivated in part by DNA methylation, and the suppression of Dab2 expression by DNA methylation may play a role in the development of myelodysplastic syndrome. PMID: 23005040
    30. These findings thereby define an adaptor-specific mechanism in the control of fibrinogen uptake and implicate that DAB2 is the key adaptor in the clathrin-associated endocytic complexes to mediate fibrinogen internalization. PMID: 22705885
    31. inhibition of Dab2 decreases phosphorylation of SMAD-1, 5, and 8 PMID: 22898784
    32. FCHO2 regulates the size of clathrin structures, and its interaction with Dab2 is needed for LDLR endocytosis under conditions of low AP2. PMID: 22323290
    33. Dab2 mediates AP-2 independent recruitment of CFTR to CCVs in polarized human airway epithelial cells. PMID: 22399289
    34. It was concluded that PKB/Akt is part of an endocytic machinery and it mediates albumin uptake through its interaction with Dab2. PMID: 22218591
    35. Results indicate that miR-187 directly targeted Disabled homolog-2 (Dab2). PMID: 21725366
    36. Dab2 is required for the TGFbeta-induced gene expression of angiogenic factors such as VEGF and FGF2 PMID: 22265793
    37. Dab2 depletion increased the CFTR half-life ~3-fold, in addition to inhibiting CFTR endocytosis. PMID: 21995445
    38. Expressions of Disabled-2 and Axin were concurrently reduced and correlated with the malignant phenotype of lung cancers. PMID: 21496867
    39. Dab2 is part of an accommodation of the cell to the altered physicochemical conditions prevalent in mitosis, aimed at allowing endocytic activity throughout the cell cycle. PMID: 21097498
    40. Loss of Dab2 expression, commonly observed in breast cancer, may facilitate TGFbeta-stimulated epithelial-to-mesenchymal transition (EMT), and therefore increase the propensity for metastasis. PMID: 21063401
    41. DAB2 acts as a tumor suppressor by dictating tumor cell TGF-beta responses, identify a biomarker for squamous cell carcinomas progression. PMID: 20592473
    42. Demonstrate frequent DAB2 promoter hypermethylation in nasopharyngeal carcinoma and support the putative tumour suppressor effect of DAB2. PMID: 20525238
    43. two pools of Dab2 co-exist at the platelet surface, in both sulfatide- and integrin receptor-bound states, and their balance controls the extent of the clotting response PMID: 19956625
    44. Data show that downregulation of myosin VI expression results in a significant reduction in PSA and VEGF secretion in LNCaP cells, and the intracellular targeting seems to involve myosin VI-interacting proteins, GIPC and LMTK2 and Dab2. PMID: 19855435
    45. mechanism of growth inhibitory effect in prostate cancer PMID: 11812785
    46. DOC2 has a role in regulating c-Src in prostatic epithelium and cancer PMID: 12473651
    47. Results suggest that disabled-2 functions as a negative regulator of canonical Wnt signaling by stabilizing the beta-catenin degradation complex. PMID: 12805222
    48. CIN85 association with Dab2 is essential for its recruitment to clathrin coat and appears to be modulated by growth factor stimulation PMID: 14596919
    49. Loss in dab-2 expression is correlated with the loss of epithelial basement membrane in morphologically normal areas PMID: 14669280
    50. a binding affinity of Dab phosphotyrosine interaction domain for megalin CT of K(D) = 2.6 x 10(-7) +/- 5.3 x 10(-8) PMID: 15134832

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  • 亚细胞定位：
    Cytoplasm. Cytoplasmic vesicle, clathrin-coated vesicle membrane. Membrane, clathrin-coated pit. Note=Colocalizes with large insert-containing isoforms of MYO6 at clathrin-coated pits/vesicles. During mitosis is progressively displaced from the membrane and translocated to the cytoplasm.
  • 组织特异性：
    Expressed in deep invaginations, inclusion cysts and the surface epithelial cells of the ovary. Also expressed in breast epithelial cells, spleen, thymus, prostate, testis, macrophages, fibroblasts, lung epithelial cells, placenta, brain stem, heart and s
  • 数据库链接：

    HGNC: 2662

    OMIM: 601236

    KEGG: hsa:1601

    STRING: 9606.ENSP00000313391

    UniGene: Hs.696631