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  4. Crotonyl-HIST1H2BC (K23) Antibody

Crotonyl-HIST1H2BC (K23) Antibody

  • 货号：
    CSB-PA010403OA23crHU
  • 规格：
    ￥660
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CSB-PA010403OA23crHU at 1:37.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate
      All lanes: HIST1H2BC antibody at 1.8μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 14 kDa
      Observed band size: 14 kDa
    • Immunoprecipitating HIST1H2BC in K562 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA010403OA23crHU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA010403OA23crHU (3μg) + K562 whole cell lysate (500μg)
      Lane 3: K562 whole cell lysate (20μg)
    • Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA23crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HIST1H2BC Polyclonal antibody
  • Uniprot No.：
    P62807
  • 基因名：
    HIST1H2BC
  • 别名：
    H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibody; H2BC6 antibody; H2BFH antibody; HIST1H2BE; antibody; H2BC7 antibody; H2BFG antibody; HIST1H2BF; antibody; H2BC8 antibody; H2BFA antibody; HIST1H2BG; antibody; H2BC10 antibody; H2BFK antibody; HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody; Histone H2B.1 A antibody; Histone H2B.a antibody; H2B/a antibody; Histone H2B.g antibody; H2B/g antibody; Histone H2B.h antibody; H2B/h antibody; Histone H2B.k antibody; H2B/k antibody; Histone H2B.l antibody; H2B/l antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence around site of Crotonyl-Lys (23) derived from Human Histone H2B type 1-C/E/F/G/I
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP, ChIP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:100-1:1000
    IF 1:20-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 数据库链接：

    HGNC: 4757

    OMIM: 602798

    KEGG: hsa:3017

    STRING: 9606.ENSP00000366962

    UniGene: Hs.182137