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Clec4g Antibody

  • 货号：
    CSB-PA803912ZA01MO
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse Heart tissue lysate
      All lanes: Clec4g antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 34 kDa
      Observed band size: 34 kDa
    • Western Blot
      Positive WB detected in: Mouse Heart tissue lysate
      All lanes: Clec4g antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 34 kDa
      Observed band size: 34 kDa
    • IHC image of CSB-PA803912ZA01MO diluted at 1:66 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-PA803912ZA01MO diluted at 1:66 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of NIH/3T3 cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of NIH/3T3 cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Mus musculus (Mouse) Clec4g Polyclonal antibody
  • Uniprot No.：
    Q8BNX1
  • 基因名：
    Clec4g
  • 别名：
    C-type lectin domain family 4 member G, Clec4g
  • 宿主：
    Rabbit
  • 反应种属：
    Mus musculus
  • 免疫原：
    Recombinant Mus musculus Clec4g protein (52-294aa)
  • 免疫原种属：
    Mus musculus (Mouse)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:30-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner.
  • 基因功能参考文献：
    1. This study shows that C-type lectin LSECtin, a member of the DC-SIGN family, is a novel liver regulator for natural killer cells (NK cells). LSECtin could bind to NK cells in a carbohydrate-dependent manner and could regulate the number of hepatic NK cells. PMID: 27184407
    2. Data show that C-type lectin-like domain family 4, member g (Clec4g) suppresses beta-Site amyloid precursor protein cleaving enzyme-1 (BACE1)-mediated amyloid-beta (Abeta) peptides generation. PMID: 25957769
    3. When expressed in B16 melanoma cells, LSECtin promoted tumor growth, whereas its blockade slowed tumor growth in either wild-type or LSECtin-deficient mice. PMID: 24769443
    4. LSECtin-mediated signaling up-regulates the threshold of CD4(+) T cell activation via tuning the expression of Cbl-b. PMID: 22884358
    5. LSECtin may facilitate the reduction of liver inflammation at the cost of delaying virus clearance, an effect that might be hijacked by the virus as an escape mechanism. PMID: 23487419
    6. The studies provide a basis for using mouse LSECtin, and knockout mice lacking this receptor, to model the biological properties of the human receptor. PMID: 21257728
    7. Liver sinusoidal endothelial cell lectin, LSECtin, negatively regulates hepatic T-cell immune response. PMID: 19632227

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 数据库链接：

    KEGG: mmu:75863

    STRING: 10090.ENSMUSP00000059574

    UniGene: Mm.109183