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CYP8B1 Antibody

  • 货号：
    CSB-PA006462LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Rat kidney tissue
      All lanes: CYP8B1 antibody at 6.4μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 59 kDa
      Observed band size: 59 kDa
    • IHC image of CSB-PA006462LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA006462LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA006462LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CYP8B1 Polyclonal antibody
  • Uniprot No.：
    Q9UNU6
  • 基因名：
  • 别名：
    7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase antibody; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase antibody; CP8B antibody; CP8B1_HUMAN antibody; CYP12 antibody; CYP8B1 antibody; CYPVIIIB1 antibody; Cytochrome P450 8B1 antibody; Cytochrome P450 subfamily VIIIB (sterol 12 alpha hydroxylase) polypeptide 1 antibody; Cytochrome P450; family 8; subfamily B; polypeptide 1 antibody; Sterol 12-alpha-hydroxylase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase protein (303-440AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CYP8B1 Antibody (CSB-PA006462LA01HU)，的标记方式是Non-conjugated。对于CYP8B1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA006462LB01HU CYP8B1 Antibody, HRP conjugated ELISA
    FITC CSB-PA006462LC01HU CYP8B1 Antibody, FITC conjugated
    Biotin CSB-PA006462LD01HU CYP8B1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis. Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH--hemoprotein reductase).
  • 基因功能参考文献：
    1. RORalpha is a key regulator of diurnal rhythm and fasting induction of CYP8B1, which regulates bile acid composition and serum and liver cholesterol levels. PMID: 24226095
    2. The single nucleotide polymorphism rs3732860 in the 3'-untranslated region of the CYP8B1 gene is associated with risk of Gallstone Disease in Chinese Han. PMID: 23216301
    3. SNP rs3732860 of CYP8B1 gene is associated with gallstone disease in this Chinese population. PMID: 22093981
    4. Cholesterol loading reduces SREBP-1 mRNA expression in addition to reducing functional SREBP-1 protein, and results in decreasing CYP8B1 gene transcription. PMID: 15249218
    5. Studies indicate a homogenous pattern for CYP8B1 expression in human liver, which was even rather than zonal. PMID: 15891895
    6. Isoflavone-related induction of 12-alpha-hydroxylase (CYP8B1) was studied in vitro and murine in vivo models. PMID: 17585019

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Liver.
  • 数据库链接：

    HGNC: 2653

    OMIM: 602172

    KEGG: hsa:1582

    STRING: 9606.ENSP00000318867

    UniGene: Hs.447793