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CUL1 Antibody

  • 货号：
    CSB-PA614436ESR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA614436ESR2HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CUL1 Polyclonal antibody
  • Uniprot No.：
    Q13616
  • 基因名：
  • 别名：
    CUL 1 antibody; CUL-1 antibody; CUL1 antibody; CUL1_HUMAN antibody; Cullin-1 antibody; Cullin1 antibody; MGC149834 antibody; MGC149835 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Cullin-1 protein (500-776AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of CCNE1, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.
  • 基因功能参考文献：
    1. High expression of CUL1 is associated with colorectal cancer. PMID: 28560438
    2. we analyzed eight probands with clinically suspected Weaver syndrome by whole-exome sequencing and identified three mutations: a 25.4-kb deletion partially involving EZH2 and CUL1 ,a missense mutation , and a missense mutation in SUZ12 inherited from her father .In vitro functional analyses demonstrated that the identified EED and SUZ12 missense mutations cause decreased decreased trimethylation of lysine 27 of H3 PMID: 28229514
    3. DCUN1D3 has a role in activating SCFSKP2 ubiquitin E3 ligase activity through cullin-1 neddylation and cell cycle progression in tumor cells with UV damage PMID: 27542266
    4. Cullin1 and MMP-2 expressions could be novel diagnostic and prognostic markers for colorectal cancer patients. PMID: 28269751
    5. Our results demonstrate that increased CUL1 expression is significantly correlated with poor prognosis of patients with osteosarcoma. CUL1 might be an important marker and a therapeutic target for osteosarcoma. PMID: 28315506
    6. Our results demonstrate that increased CUL1 expression is significantly correlated with poor prognosis of patients with urothelial carcinoma PMID: 27312089
    7. unneddylated Cul1 is another central player involved in maintenance of the adaptor module pool through the formation of Cul1-Skp-1-F-box complexes and promotion of rapid SCF assembly PMID: 28923850
    8. Data suggest that cullin1 (Cul1) may constitute a potential therapeutic target in renal cell carcinoma (RCC). PMID: 27449035
    9. Cul1 promoted mTORC1 activity and cap-dependent translation by enhancing the ubiquitination and degradation of DEPTOR. eIF4F suppression inhibited the promoting effect of Cul1 on melanoma cell proliferation. PMID: 26717892
    10. Data show that melanoma antigen, family C, 2 protein (MAGE-C2) binds with RING-box protein 1 (Rbx1) and Cullin 1, and regulates cyclin E stability in melanoma cells. PMID: 26540345
    11. High Cul1 expression predicts worse 5-year overall and disease-specific survival rates in HCC patients. PMID: 26097587
    12. Nedd8(Q40E) cannot induce the same structural effect on Cul1-Rbx1 as wild-type Nedd8. PMID: 26632597
    13. Cullin-1 has been deeply implicated in the pathogenesis and development of prostate cancer PMID: 25973042
    14. Increased CUL1 expression in CRC cells significantly promoted cell migration and invasion abilities in vitro and peritoneal metastasis in vivo through inducing high expression of MMPs. PMID: 25652886
    15. Our data indicated that Cul1 expression significantly increased in human glioma, and it may be involved in proliferation, migration and invasion of glioma cells. PMID: 25201578
    16. In papillary thyroid carcinoma, cytoplasmic expression of Cul1 was correlated with tumor occurrence, N stage and Cyclin D1 expression. Nuclear Cul1 expression was negatively correlated with tumor occurence. PMID: 24819753
    17. We discuss how these results can explain the rapid association of Cdc34 and SCF. PMID: 25425648
    18. By integrating our WES and CN data we identified three mutated putative candidate genes targeted by 7q deletions (CUL1, EZH2 and FLNC), with FLNC positioned within the well-characterized 7q minimally deleted region. PMID: 24349473
    19. data suggested that Cullin1 might promote the progression of non-small-cell lung cancer PMID: 24767980
    20. Studies indicate that in SCFs, Rbx1 serves as the RING-containing enzyme, Cul1 is the Cullin scaffold, and Skp1 is an adaptor, which serves to link the beta-TrCP F-box substrate-specific factor to the rest of the ligase. PMID: 23624913
    21. Cul1 overexpression is significantly correlated with breast cancer progression and predicts worse survival. Cul1 regulates breast cancer cell proliferation, migration and invasion. PMID: 23592700
    22. Substrate binding promotes formation of the Skp1-Cul1-Fbxl3 (SCF(Fbxl3)) protein complex. PMID: 24085301
    23. The Epstein-Barr virus protein BPLF1, is targeted to cullin-RING ubiquitin ligases (CRLs) via the interaction of the conserved helix-2 with helix-23 of cullins, at a site involved in electrostatic interaction with CAND1. PMID: 22474075
    24. CUL1 promotes human trophoblast cell invasion and dysregulation of CUL1 expression may be associated with pre-eclampsia. PMID: 23429288
    25. Skp1-Cul1-F-box ubiquitin ligase (SCF(betaTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry PMID: 23027877
    26. Deconjugation of Nedd8 from Cul1 is directly regulated by Skp1-F-box and substrate, and the COP9 signalosome inhibits deneddylated SCF by a noncatalytic mechanism. PMID: 22767593
    27. RBX1-containing cullin-RING ligases can bind glomulin (GLMN), which binds RING-box1's RING domain, regulating the RBX1-CUL1-containing SCF(FBW7) complex. PMID: 22748924
    28. Glomulin binds Rbx1 and regulates cullin-1 RING ligase-mediated turnover of Fbw7. PMID: 22405651
    29. binding of Cul1-Rbx1 to Cul7-Rbx1 is mediated via heterodimerization of Fbxw8 with other F-box proteins which function to recruit substrates into the E3 ligase complex PMID: 21946088
    30. Association with CUL1 haplotype indicates a possible role of CUL1 variation(s) in rheumatoid arthritis and its response to methotrexate. PMID: 21738080
    31. Our data indicated that Cullin1 may be an important marker for human gastric cancer lymph node metastasis and prognosis. PMID: 21190721
    32. Data show that CRN7 interacted with Cullin1 and Roc1 to form a novel SCF-like E3 complex. PMID: 21130766
    33. The Cul1 expression is significantly correlated with melanoma development at early stages, implying targeting Cul1 may serve as a potential therapy for melanoma. PMID: 20518860
    34. Cul1 regulates melanoma cell growth and cell cycle progression through degradation of p27 PMID: 20878082
    35. Rictor forms a complex with Cullin-1 to promote SGK1 ubiquitination and destruction. PMID: 20832730
    36. crystal structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF complex PMID: 11961546
    37. The COP9 signalosome inhibits p27(kip1) degradation and impedes G1-S phase progression via deneddylation of SCF Cul1. PMID: 11967155
    38. Data show that interference with Skp1 function through expression of the Cul1-N252 mutant results in the formation of multinucleated cells, centrosome and mitotic spindle abnormalities, and impaired chromosome segregation. PMID: 12417738
    39. p120(CAND1) selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation PMID: 12504025
    40. unnedyylated protein binds to CAND1 and regulates the formation of SCF ubiquitin E3 ligase complex PMID: 12504026
    41. results suggest a unique role for NEDD8-specific protease 1(DEN1) in regulating the modification of cullin 1 by Nedd8 protein PMID: 12759363
    42. regulation of CUL1 expression may affect the susceptibility of rheumatoid arthritis via altering lymphocyte signal transduction PMID: 15759013
    43. CAND1 & COP9 signalosome (CSN), major deneddylase of cullins, bind to unneddylated CUL1 in mutually exclusive way. Binding of CSN to CUL1 required 4 helix bundle in CUL1 C-terminal domain, which was wrapped around by CAND1 in CAND1-CUL1-Rbx1 complex. PMID: 16036220
    44. the SCF complex (Skp1/Cul1/F-box protein/Roc1) intervenes in the surveillance of Cdh1 cellular abundance in S-phase PMID: 16123585
    45. Results describe the regulation of neddylation and deneddylation of cullin1 by Nedd8 in SCFSkp2 ubiquitin ligase by F-box protein and substrate. PMID: 16861300
    46. Thiazolidinediones modulate the expression of beta catenin and other cell cycle proteins by targeting CUL1 independently of PPARG. PMID: 17569795
    47. CUL1 ECTD (extreme C-terminal domain; spanning the C-terminal 50 amino acids), did not contribute to CUL1's stable association with ROC1. PMID: 18723677
    48. p18-Cyclin E by the Skp1-Cul1-Fbw7 (SCF) complex and its interaction with the Fbw7 protein isoforms can take place independently of phosphorylation of p18-Cyclin E at a C-terminal phosphodegron. PMID: 18784078
    49. SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation PMID: 18826954
    50. CUL1 may function as a tumor suppressor by regulating PLK4 protein levels and thereby restraining excessive daughter centriole formation at maternal centrioles. PMID: 19679553

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  • 蛋白家族：
    Cullin family
  • 组织特异性：
    Expressed in lung fibroblasts.
  • 数据库链接：

    HGNC: 2551

    OMIM: 603134

    KEGG: hsa:8454

    STRING: 9606.ENSP00000326804

    UniGene: Hs.146806