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  4. COL8A1 Antibody

COL8A1 Antibody

  • 货号：
    CSB-PA005755LA01HU
  • 规格：
    ￥3,900
  • 图片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate
      All lanes: COL8A1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 74 kDa
      Observed band size: 74 kDa
    • Western Blot
      Positive WB detected in: Mouse brain tissue lysate, Rat heart tissue lysate, Rat brain tissue lysate
      All lanes: COL8A1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 74 kDa
      Observed band size: 74 kDa
    • IHC image of CSB-PA005755LA01HU diluted at 1:67 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence staining of Hela cell with CSB-PA005755LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of RAW264.7 cell with CSB-PA005755LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of RAW264.7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of NIH/3T3 cell with CSB-PA005755LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of NIH/3T3 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The COL8A1 polyclonal antibody was produced in rabbits using the recombinant human COL8A1 protein containing amino acids 572-744 as the immunogen. After immunization, the antibody was isolated and subjected to protein G chromatography to achieve a purity level exceeding 95%. This antibody can recognize and bind the COL8A1 protein from human and mouse samples.

    This COL8A1 polyclonal antibody has been validated in various applications, including ELISA, WB, and IF. This demonstrates the ability of the COL8A1 polyclonal antibody to detect COL8A1 protein levels and distribution in these different contexts.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) COL8A1 Polyclonal antibody
  • Uniprot No.：
    P27658
  • 基因名：
    COL8A1
  • 别名：
    C3orf7 antibody; CO8A1_HUMAN antibody; COL8A1 antibody; Collagen alpha 1(VIII) chain [Precursor] antibody; Endothelial collagen antibody; MGC9568 antibody; Vastatin antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse,Rat
  • 免疫原：
    Recombinant Human Collagen alpha-1(VIII) chain protein (572-744AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，COL8A1 Antibody (CSB-PA005755LA01HU)，的标记方式是Non-conjugated。对于COL8A1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA005755LB01HU COL8A1 Antibody, HRP conjugated ELISA
    FITC CSB-PA005755LC01HU COL8A1 Antibody, FITC conjugated
    Biotin CSB-PA005755LD01HU COL8A1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.02% sodium azide
    Constituents: PBS containing 50% glycerol pH 7.3
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC IHC：1:50-1:200，IF:1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.; Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.
  • 基因功能参考文献：
    1. COL8A1 was identified and proved to be correlated with the progression and prognosis of human colon adenocarcinoma, probably through regulating focal adhesion-related pathways. PMID: 29497907
    2. High COL8A1 level is associated with chronic obstructive pulmonary disease and cancer. PMID: 27234597
    3. The COL8A1 rs13095226 polymorphism is not associated with nAMD or PCV, which suggesting this gene maybe not a susceptibility gene locus for neovascular age-related macular degeneration (nAMD) or polypoidal choroidal vasculopathy (PCV) in Chinese subjects. PMID: 26617902
    4. High COL8A1 expression is associated with early loss of kidney function. PMID: 26110394
    5. Data indicate that complement factor H (CFH) R1210C and common variants in COL8A1 and RAD51B plus six genes contribute predictive information for advanced macular degeneration (AMD) beyond macular and behavioral phenotypes. PMID: 24498017
    6. The results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions. PMID: 23533600
    7. In addition, eight genes classified as 'second tier' hits in the original study (PAX7, THADA, COL8A1/FILIP1L, DCAF4L2, GADD45G, NTN1, RBFOX3 and FOXE1) showed evidence of linkage and association in this replication sample. PMID: 23512105
    8. The purpose of this study is to evaluate COL8A1 and COL8A2 as candidate genes for thin central corneal thickness in human primary open angle glaucoma patients. PMID: 21139683
    9. The absence of pathogenic mutations identified in the COL8A1 or COL8A2 genes in affected members of 15 pedigrees with familial FECD (Fuchs endothelial corneal dystrophy) indicates that other genetic factors are involved. PMID: 16936088
    10. The absence of pathogenic mutations in COL8A1 and COL8A2 in patients with keratoconus indicates that other genetic factors are involved in the pathogenesis of this corneal ectatic disorder. PMID: 17721297

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix, basement membrane.
  • 组织特异性：
    Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve b
  • 数据库链接：

    HGNC: 2215

    OMIM: 120251

    KEGG: hsa:1295

    STRING: 9606.ENSP00000261037

    UniGene: Hs.654548