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  4. CENPE Antibody

CENPE Antibody

  • 中文名称：
    CENPE兔多克隆抗体
  • 货号：
    CSB-PA722114
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA722114(CENPE Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA722114(CENPE Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: K562 cells, A431 cells, Primary antibody: CSB-PA722114(CENPE Antibody) at dilution 1/1400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
  • 其他：

产品详情

  • Uniprot No.：
    Q02224
  • 基因名：
    CENPE
  • 别名：
    CENPECentromere-associated protein E antibody; Centromere protein E antibody; CENP-E antibody; Kinesin-7 antibody; Kinesin-related protein CENPE antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthetic peptide of Human CENPE
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:2000-1:5000
    WB 1:500-1:2000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized. Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss.
  • 基因功能参考文献：
    1. SUMOylated NKAP is essential for chromosome alignment by anchoring CENP-E to kinetochores PMID: 27694884
    2. CENP-E motor activity appears to play important roles in the organization of midbody proteins to complete cytokinetic abscission. PMID: 27835888
    3. Data indicate that three genes, KIF14, NCAPG and CENPE that were upregulated in Pediatric high-grade gliomas (pHGGs) and were direct miR-137 or miR-6500-3p targets. PMID: 26933822
    4. CENPE was regulated by the co-binding of LSD1 and AR to its promoter, which was associated with loss of RB1 in CRPC. PMID: 28916652
    5. these results support a novel function of XAB2 in mitotic cell cycle regulation, which is partially mediated by transcription regulation on CENPE. PMID: 27735937
    6. CTCF helps recruit CENP-E to the centromere during mitosis and that it may do so through a structure stabilized by the CTCF/CENP-E complex. PMID: 26321640
    7. Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules. PMID: 25743205
    8. CENP-Q - a subunit of the CENP-O complex (comprising CENP-O, CENP-P, CENP-Q and CENP-U) that targets polo-like kinase (Plk1) to kinetochores - is also required for the recruitment of CENP-E to kinetochores. PMID: 25395579
    9. CENP-E-driven chromosome congression is guided by microtubule detyrosination. PMID: 25908662
    10. CENP-E expression is highest in basal-like subtype among breast cancer patients. PMID: 24928852
    11. An unexpected role of CENP-E elongated stalk in ensuring stability of kinetochore-microtubule attachments during chromosome congression and segregation. PMID: 24920822
    12. dynein and CENP-E at kinetochores drive congression of peripheral polar chromosomes by preventing arm-ejection forces mediated by chromokinesins from working in the wrong direction. PMID: 25383660
    13. Mutations in CENPE define a novel kinetochore-centromeric mechanism for microcephalic primordial dwarfism. PMID: 24748105
    14. A CENP-E mediated wall-tethering event and a MCAK-mediated wall-removing event show that human chromosome-microtubule attachment is achieved through a set of deterministic sequential events rather than stochastic direct capture of microtubule ends. PMID: 23891108
    15. The changes in ATP binding affinity and conformational deviations in human CENP-E motor domain, were studied. PMID: 23740391
    16. Kinetochore kinesin CENP-E is a processive bi-directional tracker of dynamic microtubule tips. PMID: 23955301
    17. In this study we investigated the pathogenic effect of 132 nsSNPs reported in CENP-E using computational platform. PMID: 22974711
    18. Evolutionarily conserved protein ERH controls CENP-E mRNA splicing and is required for the survival of KRAS mutant cancer cells. PMID: 23236152
    19. the unusually slow CENP-E microtubule association step favors CENP-E binding of stable microtubules over dynamic ones, a mechanism that would bias CENP-E binding to kinetochore fibers. PMID: 22637578
    20. Data show that CENP-E-mediated traction forces on misaligned chromosomes are responsible for the irreversible loss of spindle-pole integrity in CLASP1/2-depleted cells. PMID: 22307330
    21. SKAP cooperates with CENP-E to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation. PMID: 22110139
    22. MPS1 is required by cells to arrest in mitosis in response to spindle defects and kinetochore defects resulting from the loss of the kinesin-like protein. PMID: 12686615
    23. Aurora B allowed chromosome alignment in CENP-E-compromised cells; implied that by destabilizing pole proximal syntelic attachments, Aurora B cooperates with CENP-E to mediate congression of mono-oriented polar chromosomes PMID: 20354862
    24. An Aurora/PP1 phosphorylation switch modulates CENP-E motor activity as a feature of chromosome congression from poles and localized PP1 delivery by CENP-E to the outer kinetochore is necessary for stable microtubule capture by those chromosomes. PMID: 20691903
    25. CENP-E expression was reduced in human hepatocellular carcinoma PMID: 20021663
    26. CENP-E integrates two critical functions that are important for accurate chromosome movement and spindle architecture: one relying directly on its motor activity, and the other involving the targeting of key microtubule regulators to kinetochores. PMID: 19733075
    27. x-ray crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure PMID: 15159587
    28. Depletion of CENP-E leads to chromosomes missegregation and cell death during mitotic delay. PMID: 15181147
    29. crystal structure reveals that the CENP-E linker region is in a "docked" position identical to that in the human plus-end directed conventional kinesin PMID: 15236970
    30. Results support a plus end motion for CENP-E, based on a cryoelectron microscopy density map of the complex to 17 A resolution, which is consistent with features of the crystallographic structure. PMID: 16926026
    31. HsNUF2 and CENP-E are required for organization of stable microtubule-kinetochore attachment that is essential for faithful chromosome segregation in mitosis PMID: 17535814
    32. Global inhibition of SUMOylation caused a prometaphase arrest due to defects in targeting the microtubule motor protein CENP-E to kinetochores. PMID: 18374647
    33. Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938
    34. Data show that the kinetochore localization of PinX1 is dependent on Hec1 and CENP-E. PMID: 19553660
    35. Studies strongly suggest that chromosome congression defects as the result of KIF18A depletion is at least in part mediated through destabilizing kinetochore CENP-E. PMID: 19625775

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  • 相关疾病：
    Microcephaly 13, primary, autosomal recessive (MCPH13)
  • 亚细胞定位：
    Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family
  • 数据库链接：

    HGNC: 1856

    OMIM: 117143

    KEGG: hsa:1062

    STRING: 9606.ENSP00000265148

    UniGene: Hs.75573