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CCNY Antibody

  • 货号：
    CSB-PA847689LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate
      All lanes: CCNY antibody at 6µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 40, 37, 34 kDa
      Observed band size: 40 kDa
    • IHC image of CSB-PA847689LA01HU diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA847689LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CCNY Polyclonal antibody
  • Uniprot No.：
    Q8ND76
  • 基因名：
    CCNY
  • 别名：
    CCNY antibody; C10orf9 antibody; CBCP1 antibody; CFP1Cyclin-Y antibody; Cyc-Y antibody; Cyclin box protein 1 antibody; Cyclin fold protein 1 antibody; cyclin-X antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Cyclin-Y protein (2-170AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CCNY Antibody (CSB-PA847689LA01HU)，的标记方式是Non-conjugated。对于CCNY Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA847689LB01HU CCNY Antibody, HRP conjugated ELISA
    FITC CSB-PA847689LC01HU CCNY Antibody, FITC conjugated
    Biotin CSB-PA847689LD01HU CCNY Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription.
  • 基因功能参考文献：
    1. increased expression of CCNY was significantly associated with cell proliferation and migration. PMID: 29557391
    2. Amplification of gene CCNY is associated with metastatic lung adenocarcinoma. PMID: 28381877
    3. we believe that CCNY has biological effect in breast cancer (BC) development, and its inhibition via an RNA interference lentiviral system may provide a therapeutic option for BC PMID: 27666310
    4. CCNY was significantly upregulated in ovarian cancer cell lines and tissues. Significant association was observed between CCNY expression and clinicopathological stage and lymph node metastasis. It significantly exacerbated proliferation, migration, and invasion of A2780 cells, in vitro and in nude mice. It significantly increased c-Myc, cyclin D1, PFTK1, ki67, and OGT protein expressions in xenograft tumor tissues. PMID: 26831658
    5. Activation of PCTAIRE-1 is mediated through interaction with the phosphorylated form of cyclin Y in complex with 14-3-3. PMID: 26205494
    6. Our findings highlight a PFTK1-CCNY complex in activating noncanonical Wnt signaling in HCC cells. PMID: 24824184
    7. Human cyclin Y (CCNY) is a phosphoprotein in vivo and phosphorylation of CCNY by CDK14 triggers its ubiquitination and degradation. PMID: 24794231
    8. Cyclin Y regulates the expression and membrane trafficing of PCTAIRE kinases (PCTK). (Review) PMID: 22895054
    9. CCNY binding to CDK16 required a region upstream of the kinase domain and was found to be inhibited by phosphorylation of serine 153, a potential PKA phosphorylation site. PMID: 22184064
    10. these results validated the role of CCNY as a clinically relevant human oncoprotein PMID: 21273179
    11. CCNY might play an important role in glioma tumorigenisis. PMID: 20441050
    12. Single nucleotide polymorphism in CCNY gene is associated with Crohn's disease and ulcerative colitis PMID: 18438405

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side.; [Isoform 3]: Nucleus.
  • 蛋白家族：
    Cyclin family, Cyclin Y subfamily
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 23354

    OMIM: 612786

    KEGG: hsa:219771

    STRING: 9606.ENSP00000363836

    UniGene: Hs.14745