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CCKAR Antibody

  • 货号：
    CSB-PA004772LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate
      All lanes: CCKAR antibody at 3.4µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 48 kDa
      Observed band size: 48 kDa
    • IHC image of CSB-PA004772LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA004772LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CCKAR Polyclonal antibody
  • Uniprot No.：
    P32238
  • 基因名：
  • 别名：
    CCKAR; CCKRA; Cholecystokinin receptor type A; CCK-A receptor; CCK-AR; Cholecystokinin-1 receptor; CCK1-R
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Cholecystokinin receptor type A protein (235-313AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CCKAR Antibody (CSB-PA004772LA01HU)，的标记方式是Non-conjugated。对于CCKAR Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA004772LB01HU CCKAR Antibody, HRP conjugated ELISA
    FITC CSB-PA004772LC01HU CCKAR Antibody, FITC conjugated
    Biotin CSB-PA004772LD01HU CCKAR Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 基因功能参考文献：
    1. CCK1R may play a role differing from CCK2R in colon carcinogenesis, nuclear CCK1R represents a potential biomarker for poor prognosis. PMID: 26508021
    2. Study shows downregulation of CCKAR gene expression in A1/A1 genotype of gallstone disease patients as compared with control with significant variation in its expression pattern in relation to polymorphism. PMID: 27287528
    3. Our study showed significantly higher expression of CCKAR and down regulation of CCKBR in pancreatic cancer as compared to control while CCKBR/GR was detected in majority of stomach cancer samples. Thus, our study suggests that CCK and Gs receptors may have diagnostic and therapeutic implications. PMID: 27072272
    4. The neurotransmitter cholecystokinin (CCK), along with its receptors, CCKAR and CCKBR, have been previously associated with psychiatric disorders, suggesting that variants near these genes may play a role in the pre-pulse/startle response in this cohort PMID: 26608796
    5. CCK-AR polymorphism is protective against functional dyspepsia. PMID: 26551933
    6. There is functional synergy between cholecystokinin receptors CCKAR and CCKBR in mammalian brain development. PMID: 25875176
    7. Age related differential expression of CCKAR in GBC may suggest two possible variants of the disease in this endemic belt. PMID: 25025063
    8. Y140A mutation within a cholesterol-binding motif results in ligand binding and activity characteristics similar to wild type CCK1R. in a high cholesterol environment PMID: 24825903
    9. The findings suggest that variants in the CCKAR gene may influence the risk of gallbladder cancer in women. PMID: 23701593
    10. A significant association of the cholecystokinin-A receptor (CCKAR) gene variation rs1800857 and language lateralization, is reported. PMID: 23341962
    11. The results showed that three individual haplotypes of CCKAR were strongly associated with increased risk of schizophrenia. PMID: 22825913
    12. Data suggest that CCK-1R expression is up-regulated in kidney tubules (but not in glomeruli) in patients with diabetic nephropathy; increased expression of CCK-1R in tubules appears to be biomarker of severity of proteinuria in these patients. PMID: 22396142
    13. data may suggest that the TM3 CRAC cholesterol-binding motif could be responsible for the cholesterol sensitivity of the CCK1R. PMID: 22021636
    14. CCKAR expression was significantly increased in gallbladder cancer compared to gallstone disease. PMID: 21813391
    15. Data indicate that the Homo-Phe derivative 2 (VL-0797) enhanced 12-fold the affinity for the rat CCK(1)-R affinity and 15-fold for the human CCK(1)-R relative to the reference compound 12 (VL-0395). PMID: 21728335
    16. An association is not found between cholecystokinin A receptor polymorphisms and antipsychotic induced weight gain in schizophrenia patients. PMID: 20732371
    17. LPS can up-regulate the expression of CCK-AR and CCK-BR mRNA in vascular endothelial cells. PMID: 19751565
    18. a 2-marker haplotype (rs1800855/rs1800857) in the CCKAR gene protected women against PD (P=0.004). In addition, we found two novel rare missense variations in the CCKBR gene (Lys329Asn and Pro446Leu) in two and one patient, respectively PMID: 20023595
    19. an intron 1 polymorphism in the cholecystokinin A receptor gene is associated with schizophrenia in males PMID: 19753663
    20. Significant association between polymorphism at the -85 locus of the CCKAR gene in patients with hallucination, especially patients with hallucination in delirium tremens. PMID: 12198366
    21. the presence of CCK receptors in human ductal pancreatic tumor samples is mainly due to CCK2 expression in residual pancreatic islets and CCK1 in pancreatic nerves. PMID: 12851875
    22. heterodimerization of type A and B cholecystokinin receptors forms a powerful signaling unit with potential clinical significance in promoting cell growth PMID: 14534299
    23. In this review, both localization and functional studies suggest that the motor effects of cholecystokinin are mediated by CCK1/CCKA receptors in humans. PMID: 15100163
    24. CCK-AR gene polymorphism may be involved in the neurobiology of panic disorder. PMID: 15108185
    25. CCK-AR gene is suggested to predispose persons to schizophrenia. PMID: 15363473
    26. analysis of partial and full agonism mediated by the human cholecystokinin-1 receptor PMID: 15632187
    27. Finds significant differences in intelligence for Cholecystokinin A receptor gene promoter polymorphisms A-81G and G-128T in community-living Japanese. PMID: 15723764
    28. the deficiency of CCK-R may be a key point leading to the impairment of gallbladder motor function and the pathogenesis of cholesterol gallstone formation PMID: 15786550
    29. possible role of the CCK-AR gene in the vulnerability to schizophrenia in patients with auditory hallucinations PMID: 17413443
    30. No evidence for the association between the CCK-AR gene and schizophrenia in the Japanese population. PMID: 17413452
    31. CCK-A receptor agonist, GI181771X, did not reduce body in obese patients, suggesting that CCK-A by itself does not have a central role in long-term energy balance. PMID: 17597711
    32. Responses of human esophageal sphincter sling and clasp fibers to cholecystokinin (CCK) and gastrin through CCK-A and -B receptors are reported. PMID: 18444993
    33. Report effects of cholecystokinin-58 on type 1 cholecystokinin receptor function and regulation. PMID: 18776046

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 数据库链接：

    HGNC: 1570

    OMIM: 118444

    KEGG: hsa:886

    STRING: 9606.ENSP00000295589

    UniGene: Hs.129