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  4. CCAR2 Antibody

CCAR2 Antibody

  • 货号：
    CSB-PA040106
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    Q8N163
  • 基因名：
    CCAR2
  • 别名：
    CCAR2 antibody; DBC1 antibody; KIAA1967Cell cycle and apoptosis regulator protein 2 antibody; Cell division cycle and apoptosis regulator protein 2 antibody; DBIRD complex subunit KIAA1967 antibody; Deleted in breast cancer gene 1 protein antibody; DBC-1 antibody; DBC.1 antibody; NET35 antibody; p30 DBC antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human DBC-1.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Mediates ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation. Represses the ligand-dependent transcriptional activation function of ESR2. Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1. Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization. Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway. Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3. Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2. Represses the transcriptional activator activity of BRCA1. Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro.
  • 基因功能参考文献：
    1. Since its up-regulation in cancer patients is usually associated with poor prognosis and its depletion reduces cancer cell growth in vitro, CCAR2 was suggested to act as a tumor promoter. However, there is also evidence that CCAR2 functions as a tumor suppressor and therefore its role in cancer formation and progression is still unclear. Review. PMID: 29807573
    2. CCAR2/DBC1 inhibits recombination by limiting the initiation and the extent of DNA end resection, thereby acting as an antagonist of CtIP. PMID: 27503537
    3. conclude, these findings demonstrated that DBC1 was essential in tumorigenesis and proliferation. Moreover, it was identified as a potential therapeutic target for HCC. PMID: 29106957
    4. Long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation. PMID: 28973437
    5. Data suggest that DBC1 has a dual function in regulating beta-catenin-PROX1 signaling axis: as a coactivator for both beta-catenin and PROX1. PMID: 26477307
    6. These results establish an important role for CCAR2 in cancer cells proliferation and could shed new light on novel therapeutic strategies against cancer, devoid of detrimental side effects. PMID: 27809307
    7. important role for CCAR2 in maintaining cell cycle progression and promoting squamous cell carcinoma (SCC) tumorigenesis. PMID: 27725203
    8. Data show that the interaction between cell cycle and apoptosis regulator 2 (CCAR2) and heat shock protein 60 (Hsp60) increases in the presence of rotenone. PMID: 28254432
    9. Results found transcriptional levels of DBC1,a negative regulator of HDAC3 significantly reduced in type 2 diabetes mellitus patients. PMID: 27904654
    10. DBC1 protein could be a prognostic marker of shorter recurrence-free survival in hepatocellular carcinoma patients after hepatectomy and human hepatocarcinogenesis was a multistep process accompanied by a stepwise increase in high DBC1 expression from low-grade dysplastic nodules, through high-grade dysplastic nodules, to hepatocellular carcinoma. PMID: 27083241
    11. Results suggest that DBC1 is integral to the maintenance of the circadian molecular clock. PMID: 26657080
    12. loss of DBC1 expression plays a role in tumorigenesis and tumor progression in gallbladder carcinoma. PMID: 26617872
    13. Proteosome-mediated degradation and poly-ubiquitination of AR were increased with the knock-down of DBC1. PMID: 26249023
    14. These studies further extend and confirm the role of CCAR2 in the DNA damage response and DNA repair PMID: 26158765
    15. The results suggest that the PP4-mediated dephosphorylation of DBC1 is necessary for efficient damage responses in cells. PMID: 26194823
    16. These results indicate that the expression of DBC1 and BRCA1 are closely related with in the progression of ovarian carcinomas PMID: 25823848
    17. the results indicated that DBC1 promotes anoikis resistance in gastric cancer cells by regulating NF-kappaB activity and may thus be a new therapeutic target for preventing potential metastasis PMID: 26035299
    18. DBC1 modification by Small Ubiquitin-like Modifier 2/3 is crucial for p53 transactivation under genotoxic stress. PMID: 25406032
    19. molecular mechanism underlying DBC1 function in PEA3-mediated transcription involves inhibition of SIRT1 interaction with PEA3 and of SIRT1-mediated deacetylation of PEA3 PMID: 25417701
    20. DBC1 might promote adipose tissue inflammation and senescence in obese subjects PMID: 25682741
    21. Although DBC1 gene expression was reduced in adipose tissue from obese subjects, it was negatively associated with ADIPOQ gene expression in VAT, suggesting that DBC1 might promote visceral adipose tissue dysfunction. PMID: 25648830
    22. These results clearly indicate a novel mechanism in which CCAR2 may regulate the transcriptional activation function of LXRalpha due to its specific inhibition of SIRT1 and serve to regulate cellular proliferation. PMID: 25661920
    23. We propose that DBC1 is part of the molecular machinery that regulates fat storage capacity in adipocytes and participates in the "turn-off" switch that limits adipocyte fat accumulation. PMID: 25053585
    24. CK2 alpha is an independent prognostic indicator for gastric carcinoma patients and is involved in tumorigenesis by regulating the phosphorylation of DBC1. PMID: 24962073
    25. The results link Chk2 and REGgamma to the mechanism underlying the DBC1-dependent SIRT1 inhibition. PMID: 25361978
    26. cytoplasmic MCC-DBC1 interaction sequesters DBC1 away from the nucleus, thereby removing a brake on DBC1 nuclear targets, such as SIRT1 PMID: 24824780
    27. These results suggest that DBC1 is over-expressed in colorectal cancer and that it might serve as a predictor for selecting patients at high risk of poor prognosis. PMID: 23299276
    28. demonstrates that SIRT1- and DBC1-related pathways may be involved in the progression of soft-tissue sarcomas and can be used as clinically significant prognostic indicators for sarcoma patients PMID: 24019980
    29. Results reveal novel mechanisms by which TFII-I and DBC1 can modulate cellular fate by affecting cell-cycle control as well as the homologous recombination pathway. PMID: 24231951
    30. DBC1 as a novel regulator of gluconeogenesis. PMID: 24415752
    31. DBC1 may be implicated in the regulation of cancer cell energy metabolism. [Review] PMID: 23841676
    32. This study demonstrates that the acetylation status of P53 and the expression of SIRT1, DBC1, and AR could be new prognostic indicators for clear cell renal cell carcinoma PMID: 24018803
    33. Protein acetylation serves as an endogenous regulatory mechanism for SIRT1-DBC1 binding. PMID: 23892437
    34. MOF acetylation of DBC1 inhibits binding to SirT1 and serves as a mechanism that connects DNA damage signaling to SirT1 and cell fate determination. PMID: 24126058
    35. Our findings integrate KSR1 into a network involving DBC1 and SIRT1, which results in the regulation of p53 acetylation and its transcriptional activity. PMID: 24129246
    36. DBC1 is an important co-factor for the control of the IKK-beta-NF-kappaB signaling pathway that regulates anoikis. PMID: 23588592
    37. DBC1 enhances cell survival against UV irradiation.Therefore, DBC1 plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. PMID: 23352644
    38. DBC1 phosphorylation by ATM/ATR inhibits SIRT1 deacetylase in response to DNA damage. PMID: 22735644
    39. Data indicate that an increase in cAMP/PKA activity resulted in the dissociation of SIRT1 and DBC1 in an AMP-activated protein kinase (AMPK)-dependent manner. PMID: 22553202
    40. data indicate that the DBIRD complex (consisting of DBC1 and ZNF326) acts at the interface between mRNP particles and RNAPII, integrating transcript elongation with the regulation of alternative splicing PMID: 22446626
    41. Suggest that DBC1 may promote tumor progression, and DBC1 could be a prognostic biomarker in esophageal squamous cell carcinoma. PMID: 22127596
    42. results implicate the principal role of DBC1 in regulating ERbeta-dependent gene expressions PMID: 20074560
    43. HDAC3 is negatively regulated by the nuclear protein DBC1 PMID: 21030595
    44. DBC1 may modulate the cellular functions of BRCA1 and have important implications in the understanding of carcinogenesis in breast tissue. PMID: 20160719
    45. New role for DBC1 as an in vivo regulator of SIRT1 activity and liver steatosis, in which interaction with SIRT1 may serve as a new target for therapies aimed at nonalcoholic liver steatosis. PMID: 20071779
    46. Expression of DBC1 and SIRT1 is a significant prognostic indicator for gastric carcinoma patients. PMID: 19509139
    47. Results identify DBC1 as a novel cellular inhibitor of SUV39H1 activity, and suggest that DBC1 may be an important regulator of heterochromatin formation and genomic stability by disrupting the SUV39H1-SirT1 complex and inactivating both enzymes. PMID: 19218236
    48. Caspase-dependent processing of DBC-1 may act as a feed-forward mechanism to promote apoptosis and possibly also tumor suppression. PMID: 15824730
    49. biological function for DBC-1 in the modulation of ERalpha expression and hormone-independent breast cancer cell survival PMID: 17473282
    50. DBC1 directly interacts with SIRT1 and inhibits SIRT1 activity in vitro and in vivo PMID: 18235501

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle.
  • 组织特异性：
    Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level). Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.
  • 数据库链接：

    HGNC: 23360

    OMIM: 607359

    KEGG: hsa:57805

    STRING: 9606.ENSP00000310670

    UniGene: Hs.744848