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CASP4 Antibody

  • 货号：
    CSB-PA060273
  • 规格：
    ￥880
  • 其他：

产品详情

  • Uniprot No.：
    P49662
  • 基因名：
  • 别名：
    Apoptotic cysteine protease Mih1/TX antibody; CASP-4 antibody; CASP4 antibody; CASP4_HUMAN antibody; Caspase 4 apoptosis related cysteine peptidase antibody; Caspase-4 subunit 2 antibody; Caspase4 antibody; ICE(rel)-II antibody; ICE(rel)II antibody; ICEREL II antibody; ICH2 antibody; Mih1/TX antibody; Protease ICH-2 antibody; Protease TX antibody; TX antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human Caspase-4.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:40000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS. Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1. Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators. Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity. Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains. Activated by direct binding to LPS without the need of an upstream sensor. Does not directly process IL1B. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.
  • 基因功能参考文献：
    1. Results implicate pyroptosis induced by the CASP11/4-GSDMD pathway in the pathogenesis of alcoholic hepatitis PMID: 29108122
    2. Results indicate functional differences between human caspase-4 and murine caspase-11. PMID: 29339744
    3. The last four amino acids of the NleF carboxy terminus are essential in inhibiting caspase-4-dependent inflammatory cell death. PMID: 28593173
    4. Pathophysiological studies showed that the overexpression of the CASP4 gene was involved in the loss of proximal tubules and renal injury in nephropathic cystinosis patients. Considering kidney's key roles in sodium filtration and reabsorption CASP4 genes are involved in Blood Pressure regulation. PMID: 27271309
    5. We propose that microglial caspase-4 expression contributes to the cognitive impairments in Alzheimer's disease (AD), and that further study of caspase-4 will enhance our understanding of AD pathogenesis and may lead to novel therapeutic targets in AD. PMID: 27516385
    6. this study shows that activation of caspase-4 is mediated by interactions with endotoxin-rich membrane interfaces rather than by endotoxin monomers PMID: 28409545
    7. apoptosis is induced by rhein traditional Chinese medicine via induction of endoplasmic reticulum stress, caspase-4 and intracellular calcium in primary human hepatic HL-7702 cells PMID: 27003256
    8. Caspase-4 and caspase-5 mediate IL-1alpha and IL-1beta release from human monocytes after lipopolysaccharides stimulation. PMID: 26508369
    9. both caspase-4 and caspase-5 are functionally important for appropriate responses to intracellular Gram-negative bacteria. PMID: 26173988
    10. caspase-4 activation alone is sufficient to induce pyroptosis, this process depends on the NLRP3 inflammasome activation to drive IL-1beta maturation. PMID: 26174085
    11. This study reveled that CASP4 having a central role in the bipolar disease and schizophrenia manifestation. PMID: 25487697
    12. NF-kappaB can mediate Fas-induced apoptosis through caspase-4 protease PMID: 25695505
    13. implicate caspase-4 as a critical regulator of noncanonical inflammasome activation that initiates defense against bacterial pathogens in primary macrophages PMID: 25964352
    14. this study has identified epithelial-expressed caspases-4 and -5 as biomarkers with diagnostic and therapeutic potential in colorectal cancer. PMID: 25943872
    15. s observed increases in the endoplasmic reticulum stress markers BiP and cleaved caspase-4 in Duchenne muscular dystrophy patient biopsies, compared with controls. PMID: 24879640
    16. The caspase-4/11 inflammasome also governs activation of the proinflammatory cytokine, interleukin (IL)-18, in response to intracellular and extracellular enteric pathogens. PMID: 25121752
    17. function of caspase-4/5/11 represents a new mode of pattern recognition in immunity and also an unprecedented means of caspase activation PMID: 25119034
    18. caspase-4 can support activation of caspase-1 and secretion of IL-1beta and IL-18 in response to priming signals (LPS or Pam3CSK4) alon PMID: 24879791
    19. TLR3 stimulation in keratinocytes induces a caspase-4 dependent release of pro-IL-1beta, but further processing to active IL-1beta is limited. Furthermore, TLR3 stimulation results in pyroptotic- and apoptotic cell death. PMID: 23845419
    20. Shigella OspC3 interacts with the caspase-4-p19 subunit and inhibits its activation by preventing caspase-4-p19 and caspase-4-p10 heterodimerization. PMID: 23684308
    21. TMEM214 is essential for endoplasmic reticulum (ER) stress-induced apoptosis by acting as an anchor for recruitment of procaspase 4 to the ER and its subsequent activation. PMID: 23661706
    22. Loss of heterozygosity contributes to tumor progression of oral squamous cell carcinoma, and a specific role for PDCD4, CTNNB1, and CASP4 was found. PMID: 21781452
    23. These experiments identify caspase 4 as a novel regulator of TNF-alpha-induced NF-kappaB signaling that is required for the activation of I-kappaB kinase. PMID: 22733992
    24. Caspase-4 expression is essential for efficient nucleotide-binding domain leucine-rich repeat containing, Pyrin domain containing-3 and for absent in melanoma 2 inflammasome-dependent proIL-1beta activation in macrophages PMID: 22246630
    25. Levels of c-Rel directly modulated expression of caspase-4 as well as other endoplasmic reticulum stress genes. PMID: 21984918
    26. Data suggest that Casp4, Casp 6 and TNFSF10 are differentially expressed in potentially fertile and subfertile men and represent useful biomarkers for predicting male fertility in combination with P1 and P2. PMID: 21317160
    27. results suggest that caspase-4 directly activates caspase-9 by the processing of procaspase-9 at Asp-315 in endoplasmic reticulum stress-induced neuronal apoptosis PMID: 21282934
    28. Neuronal cell death is induced via cleavage of caspase-4 in endoplasmic reticulum cells under stress in vitro. PMID: 20816908
    29. This study suggests a possible role of caspase-4 in the apoptotic loss of proximal tubular cells. PMID: 19705160
    30. results show that activation of caspase-4 contributes to TRAIL-induced apoptosis and is associated with induction of ER stress by TRAIL in melanoma cells PMID: 20514521
    31. Caspase-4 can function as an ER stress-specific caspase in humans, and may be involved in pathogenesis of Alzheimer's disease. PMID: 15123740
    32. caspase-12 and caspase-4 are not required for the induction of ER stress-induced apoptosis and that caspase-4-like activity is not always associated with an initiating event. PMID: 15975932
    33. the familial Alzheimer's disease-linked presenilin 1 mutation accelerates the cleavage of caspase-4 under the endoplasmic reticulum stress PMID: 17942194
    34. These results demonstrate that induction of GADD153/CHOP plays a pivotal role in mechanism of ER stress-induced cell death in SH-SY5Y cells, on the other hand, cleavage of pro-caspase-4 by activation of calpain play a crucial role in SK-N-SH cells. PMID: 18029041
    35. TRAF6-caspase-4 interaction, triggered by LPS, leads to NF-kappaB-dependent transcriptional up-regulation and secretion of important cytokines and chemokines in innate immune signaling in human monocytic cells. PMID: 18056395
    36. somatic mutation of the CASP4 gene is common in gastrointestinal stromal tumor & suggest a possibility that CASP4 mutation might lead to alteration of apoptotic or inflammatory function & contribute to pathogenesis of some gastrointestinal stromal tumors PMID: 19269008

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  • 亚细胞定位：
    Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion. Inflammasome. Secreted.
  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.
  • 数据库链接：

    HGNC: 1505

    OMIM: 602664

    KEGG: hsa:837

    STRING: 9606.ENSP00000388566

    UniGene: Hs.138378