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CASP2 Antibody

  • 货号：
    CSB-PA004547LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA004547LA01HU diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA004547LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CASP2 Polyclonal antibody
  • Uniprot No.：
    P42575
  • 基因名：
  • 别名：
    CASP 2 antibody; CASP-2 antibody; Casp2 antibody; CASP2_HUMAN antibody; Caspase 2 antibody; Caspase 2 apoptosis related cysteine peptidase antibody; Caspase-2 subunit p12 antibody; Caspase2 antibody; ICH 1 antibody; ICH 1 protease antibody; ICH 1L antibody; ICH1 antibody; ICH1 protease antibody; ICH1L antibody; NEDD-2 antibody; NEDD2 antibody; Neural precursor cell expressed developmentally down-regulated protein 2 antibody; PPP1R57 antibody; Protease ICH-1 antibody; Protein phosphatase 1 regulatory subunit 57 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Caspase-2 protein (2-452AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CASP2 Antibody (CSB-PA004547LA01HU)，的标记方式是Non-conjugated。对于CASP2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA004547LB01HU CASP2 Antibody, HRP conjugated ELISA
    FITC CSB-PA004547LC01HU CASP2 Antibody, FITC conjugated
    Biotin CSB-PA004547LD01HU CASP2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:1000-1:2000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress.
  • 基因功能参考文献：
    1. these data identify a novel caspase-2-interacting factor, FAN, and expand the role for the enzyme in seemingly non-apoptotic cellular mechanisms. PMID: 29621545
    2. Results suggest that TG-induced macrophage cell death is mediated via the caspase-2. PMID: 28768565
    3. study demonstrates that apoptosis inhibitor 5 (API5/AAC11) is an endogenous and direct inhibitor of caspase-2. API5 protein directly binds to the caspase recruitment domain (CARD) of caspase-2 and impedes dimerization and activation of caspase-2. PMID: 28336776
    4. This peptide, Ac-VDTTD-AFC, was efficiently cleaved by purified caspase-2 and auto-activating caspase-2 in mammalian cells, and exhibited better selectivity for caspase-2 relative to caspase-3 than reagents that are currently available. PMID: 27919034
    5. There results support a special role for miR-149 in malignant glioma by targeting Caspase-2 PMID: 27049919
    6. Whole exome sequencing (WES) of an affected fetus, and subsequent Sanger sequencing of the second fetus, revealed a homozygous frameshift variant in CRADD, which encodes an adaptor protein that interacts with PIDD and caspase-2 to initiate apoptosis PMID: 28686357
    7. This study shows that human procaspase-2 interaction with 14-3-3 zeta is governed by phosphorylation at both S139 and S164. PMID: 28943433
    8. NPM1-dependent nucleolar PIDDosome is a key initiator of the caspase-2 activation cascade. PMID: 28432080
    9. Sensitization of colon carcinoma cells to radiation-induced cell death and DNA-damage by HuR knockdown critically depends on caspase-2. PMID: 28219770
    10. BCL9L dysfunction contributes to aneuploidy tolerance in both TP53-WT and mutant cells by reducing basal caspase-2 levels and preventing cleavage of MDM2 and BID. PMID: 28073006
    11. CASP2 down-regulation had a reverse relationship with miR-383 down-regulation in regulating epithelial ovarian cancer development. PMID: 27567588
    12. Results suggest that mutations at all three cleavage sites of caspase-2 protein neither affect the macromolecular core complex assembly, nor modify caspase-2 activity upon DNA damage. Consequently, caspase-2 activation occurs in the macromolecular complex without its dissociation. PMID: 27193717
    13. These findings indicate that miR-125a-5p decreases after HOTAIR knockdown to promote cancer cell apoptosis by releasing caspase 2. PMID: 26962687
    14. these studies elucidate a Caspase-2-p53 signaling network that impacts lung tumorigenesis and chemotherapy response in vivo. PMID: 25301067
    15. We have also demonstrated that these correlations are tissue specific being reduced (CASP9 and CASP10) or different (CASP2) in the liver PMID: 25330190
    16. the initiator caspase-2 is required for robust death of ovarian cancer cells induced by FASN inhibitors PMID: 25151963
    17. HuR sensitizes adenocarcinoma cells to the intrinsic apoptotic pathway by upregulating the translation of caspase-2. PMID: 25010987
    18. s have demonstrated in vitro and in vivo that loss of function of caspase-2 allows to escape oncogenic stress induced senescence. PMID: 25114039
    19. Data strongly argue against a critical role for caspase-2 in ER-stress-induced apoptosis. PMID: 24292555
    20. axon regeneration promoted by suppression of CASP2 and CASP6 is CNTF-dependent and mediated through the JAK/STAT signalling pathway PMID: 24727569
    21. Our results reveal a novel mechanism of caspase-2 pre-mRNA splicing. PMID: 24321384
    22. TRIM16 can promote apoptosis by directly modulating caspase-2 activity in cancer cells. PMID: 23404198
    23. The role of caspase-2 isoforms in the progression of breast cancer may considerably differ between pre- and post-chemotherapy patients. PMID: 23469978
    24. MiR-708 may act as an oncogene and induce the carcinogenicity of bladder cancer by down-regulating Caspase-2 level. PMID: 23568547
    25. caspase-2 has a role as an initiator caspase in lipoapoptosis PMID: 23553630
    26. Data indicate induction of caspase-2 by sorting nexin 5 (SNX5) in papillary thyroid carcinoma PMID: 22486813
    27. activated human caspase-2 shares remarkably overlapping protease specificity with the prototype apoptotic executioner caspases-3 and -7, suggesting that caspase-2 could function as a proapoptotic caspase once released from the activating complex. PMID: 22825847
    28. IRE1alpha regulates translation of a proapoptotic protein, Caspase-2, through terminating microRNA biogenesis, and noncoding RNAs are part of the ER stress response PMID: 23042294
    29. These results revealed a thus far unknown, obligatory role for caspase-2 as an initiator caspase during pore-forming toxins -mediated apoptosis. PMID: 22531785
    30. caspase-2 acts upstream of caspase-3 and that caspase-2 functions in response to DNA damage in both PhSe-T- and MeSe-T-induced apoptosis. PMID: 22002103
    31. Tumor-suppressing function of caspase-2 requires catalytic site Cys-320 and site Ser-139 in mice. PMID: 22396545
    32. TAp73alpha represses caspase-2 enzymatic activity and by this means reduce caspase-2 induced Bax activation, loss of mitochondrial transmembrane potential and resulting apoptosis in small cell lung carcinoma cells. PMID: 22201672
    33. Data suggest that this novel role of caspase-2 as a translational regulator of p21 expression occurs not only independently of its enzymatic activity but also does not require known caspase-2-activating platforms. PMID: 21475302
    34. Findings suggest that XPC enhances DNA damage-induced apoptosis through inhibition of caspase-2 (casp-2S) transcription. PMID: 22174370
    35. Studies indicate that DNA damage may trigger caspase 2 activation. PMID: 22077397
    36. somatic mutation of caspase-2 is rare in gastric and colorectal carcinomas. PMID: 21940110
    37. ras-induced down-regulation of caspase-2 represents a novel mechanism by which oncogenic Ras protects malignant intestinal epithelial cells from anoikis PMID: 21903589
    38. Structural and enzymatic insights into caspase-2 protein substrate recognition and catalysis. PMID: 21828056
    39. Caspase-2 directly cleaves the E3 ubiquitin ligase Mdm2 at Asp 367, leading to loss of the C-terminal RING domain responsible for p53 ubiquitination. PMID: 21726810
    40. analyzed cancer tissues from acute leukemias, breast cancers, lung cancers, and liver cancers for the detection of caspase-2 somatic mutations PMID: 21332795
    41. The changes of caspase-2 and caspase-5 activities could be indicative of their involvement in the cervical malignancy mechanisms. PMID: 21051981
    42. Activation of caspase-9, but not caspase-2 or caspase-8, is essential for heat-induced apoptosis in Jurkat cells. PMID: 20978129
    43. This review discusses recent advances that have been made to help elucidate the true role of caspase 2 and the potential contribution of caspase-2 to the pathology of human diseases including cancer. PMID: 20158568
    44. caspase-2 activation is commonly associated with induction of IFN-beta-induced apoptosis in IFN-beta-sensitive melanoma cells PMID: 20187765
    45. we suggest that caspase-2 and/or -8 plays an important role in regulating gamma-secretase and may possibly provide a basis for the development of therapeutics targeting apoptosis PMID: 20143425
    46. The expressions of PIDD and RAIDD are upregulated during tumour progression in renal cell carcinomas. PMID: 20208132
    47. caspase 2 functions as an endogenous inhibitor of NFkappaB-dependent cell survival and this mechanism may contribute to tumor suppression in humans. PMID: 19935698
    48. Data show that the N-t-boc-Daidzein induced apoptosis is characterized by caspase activation, XIAP and AKT degradation. PMID: 19738422
    49. Data indicate that caspase activity was not essential for docetaxel-induced cytotoxicity since cell death associated with lysosomal membrane permeabilization still occurred in the presence of caspase inhibitors. PMID: 19715609
    50. role in cytochrome C release and apoptosis from the nucleus PMID: 11823470

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  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • 数据库链接：

    HGNC: 1503

    OMIM: 600639

    KEGG: hsa:835

    STRING: 9606.ENSP00000312664

    UniGene: Hs.368982