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CARMIL2 Antibody

  • 货号：
    CSB-PA739364LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate
      All lanes: CARMIL2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 155, 149 kDa
      Observed band size: 155 kDa
    • Immunoprecipitating CARMIL2 in Jurkat whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA739364LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA739364LA01HU (6μg) + Jurkat whole cell lysate (500μg)
      Lane 3: Jurkat whole cell lysate (20μg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CARMIL2 Polyclonal antibody
  • Uniprot No.：
    Q6F5E8
  • 基因名：
    CARMIL2
  • 别名：
    CARMIL2 antibody; CARMIL2b antibody; Leucine rich repeat containing 16C antibody; leucine-rich repeat antibody; Leucine-rich repeat-containing protein 16C antibody; LR16C_HUMAN antibody; LRRC16C antibody; RGD antibody; RGD leucine rich repeat tropomodulin and proline rich containing protein antibody; RGD motif leucine rich repeats tropomodulin domain and proline rich containing antibody; Rltpr antibody; tropomodulin and proline-rich-containing protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Capping protein, Arp2/3 and myosin-I linker protein 2 protein (1136-1290AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CARMIL2 Antibody (CSB-PA739364LA01HU)，的标记方式是Non-conjugated。对于CARMIL2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA739364LB01HU CARMIL2 Antibody, HRP conjugated ELISA
    FITC CSB-PA739364LC01HU CARMIL2 Antibody, FITC conjugated
    Biotin CSB-PA739364LD01HU CARMIL2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization. Plays a role in cell protrusion formations; involved in cell polarity, lamellipodial assembly, membrane ruffling and macropinosome formations. Involved as well in cell migration and invadopodia formation during wound healing. Required for CD28-mediated stimulation of NF-kappa-B signaling, involved in naive T cells activation, maturation into T memory cells, and differentiation into T helper and T regulatory cells.
  • 基因功能参考文献：
    1. These cases showed that CARMIL2-deficiency is an autosomal recessive primary immunodeficiency disorder associated with defective CD28-mediated TCR co-signalling and impaired cytoskeletal dynamics. PMID: 28112205
    2. RLTPR (p.Q575E) increases binding of RLTPR to downstream components of the NF-kappaB signaling pathway, selectively upregulates the NF-kappaB pathway in activated T cells. PMID: 28694326
    3. the scaffolding role of RLTPR predominates during CD28 co-stimulation and underpins the similar function of RLTPR in human and mouse T cells. PMID: 27647348
    4. Human RLTPR deficiency is a Combined immunodeficiency affecting at least the CD28-responsive pathway in T cells and the BCR-responsive pathway in B cells. PMID: 27647349
    5. On the basis of these results, we propose a model in which dynamic vimentin filaments target CARMIL2 to critical membrane-associated locations, where CARMIL2 regulates CP, and thus actin assembly, to create cell protrusions PMID: 26466680
    6. Data suggest that CARMIL2 displays two localization patterns in cells; one coincides with vimentin intermediate filaments, and the other coincides with cell membrane at leading edge of migrating cells. PMID: 26578515

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  • 亚细胞定位：
    [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cell projection, ruffle.
  • 蛋白家族：
    CARMIL family
  • 组织特异性：
    Expressed in all tissues tested, including thymus, spleen, colon, leukocytes, peripheral blood, skin, skin keratinocytes and skin fibroblasts. Strong expression is detected in naive and memory CD4+ and CD8+ T cells, naive and memory B cells, regulatory T
  • 数据库链接：

    HGNC: 27089

    OMIM: 610859

    KEGG: hsa:146206

    STRING: 9606.ENSP00000334958

    UniGene: Hs.611432