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CAPRIN1 Antibody

  • 货号：
    CSB-PA614529LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA614529LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA614529LA01HU diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA614529LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CAPRIN1 Polyclonal antibody
  • Uniprot No.：
    Q14444
  • 基因名：
    CAPRIN1
  • 别名：
    activation/proliferation-associated protein 1 antibody; CAPR1_HUMAN antibody; Caprin-1 antibody; CAPRIN1 antibody; Cell cycle-associated protein 1 antibody; Cytoplasmic activation- and proliferation-associated protein 1 antibody; cytoplasmic activation/proliferation-associated protein-1 antibody; GPI-anchored membrane protein 1 antibody; GPI-anchored protein p137 antibody; GPI-p137 antibody; GPIAP1 antibody; GPIP137 antibody; M11S1 antibody; Membrane component chromosome 11 surface marker 1 antibody; p137GPI antibody; RNA granule protein 105 antibody; RNG105 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Caprin-1 protein (573-703AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CAPRIN1 Antibody (CSB-PA614529LA01HU)，的标记方式是Non-conjugated。对于CAPRIN1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA614529LB01HU CAPRIN1 Antibody, HRP conjugated ELISA
    FITC CSB-PA614529LC01HU CAPRIN1 Antibody, FITC conjugated
    Biotin CSB-PA614529LD01HU CAPRIN1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Binds directly and selectively to MYC and CCND2 RNAs. In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs.
  • 基因功能参考文献：
    1. that high Caprin1 expression was significantly associated with worse overall survival for patients with hepatocellular carcinoma PMID: 29037839
    2. Based on insights from the structures and existing biochemical data, the existence of an evolutionarily conserved ribonucleoprotein (RNP) complex consisting of Caprin-1, FMRP and G3BP1 is proposed. PMID: 27303792
    3. Data suggest that DEAD-box helicase 3 (DDX3X) physically interacts and co-localizes with poly(A)-binding cytoplasmic protein 1 (PABPC1) and caprin-1 in lamellipodia at the leading edge of spreading cells; these interactions are dependent on mRNA; depletion of DDX3X (via gene silencing with the CRISPR-Cas system) leads to decreased cell motility. These studies were conducted using MRC5 lung fibroblast cell line. PMID: 28733330
    4. G3BP mediates the condensation of stress granules by shifting between two different states that are controlled by the phosphorylation of S149 and by binding to Caprin1 or USP10. PMID: 27022092
    5. The G3BP1-Caprin1-PKR complex represents a new mode of PKR activation and is important for antiviral activity of G3BP1 and PKR during infection with mengovirus. PMID: 25784705
    6. Data show that tylophorine compounds exert anti-cancer activity predominantly by targeting and sequestering the caprin-1 protein and c-Myc mRNA associated ribonucleoprotein complex. PMID: 25669982
    7. G3BP1, G3BP2 and CAPRIN1 are required for translation of interferon stimulated mRNAs and are targeted by a dengue virus non-coding RNA. PMID: 24992036
    8. Cyr61/Caprin-1 co-expression was associated with worse survival. PMID: 23528710
    9. These results suggest that the Japanese encephalitis virus core protein circumvents translational shutoff by inhibiting stress granule formation through an interaction with Caprin-1 and facilitates viral propagation in vitro and in vivo. PMID: 23097442
    10. Fragile mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes PMID: 22737234
    11. Homologous region-1 (HR-1) is a novel protein domain that has been identified near the N-terminus of human caprin-1; highly conserved in vertebrates and insects, it is also present in the human caprin-2 paralog. PMID: 14764709
    12. involvement of cellular protein p137 in transcription of intermediate stage genes may regulate the transition between early and late phases of vaccinia virus replication PMID: 15471883
    13. Suppression of expression of human Caprin-1 results in slowing of the proliferation rate, due to prolongation of the G1 phase of the cell cycle, formally demonstrating that Caprin-1 is essential for normal cellular proliferation. PMID: 16177067
    14. Caprin-1/G3BP-1 complex is likely to regulate the transport and translation of mRNAs of proteins involved with synaptic plasticity in neurons PMID: 17210633
    15. MiR-16 negatively regulate HMGA1 and caprin-1 which are involved in cell proliferation. PMID: 19250063

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  • 亚细胞定位：
    Cytoplasm, cytosol. Cell projection, dendrite. Cell projection, lamellipodium.
  • 蛋白家族：
    Caprin family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 6743

    OMIM: 601178

    KEGG: hsa:4076

    STRING: 9606.ENSP00000340329

    UniGene: Hs.471818