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CA4 Antibody

  • 货号：
    CSB-PA004372LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA004372LA01HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA004372LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CA4 Polyclonal antibody
  • Uniprot No.：
    P22748
  • 基因名：
    CA4
  • 别名：
    CA IV antibody; CA4 antibody; CAH4_HUMAN antibody; CAIV antibody; Car4 antibody; Carbonate dehydratase IV antibody; Carbonic anhydrase 4 antibody; Carbonic dehydratase antibody; Carbonic dehydratase IV antibody; EC 4.2.1.1 antibody; Retinitis pigmentosa 17 (autosomal dominant) antibody; RP17 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Carbonic anhydrase 4 protein (75-200AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CA4 Antibody (CSB-PA004372LA01HU)，的标记方式是Non-conjugated。对于CA4 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA004372LB01HU CA4 Antibody, HRP conjugated ELISA
    FITC CSB-PA004372LC01HU CA4 Antibody, FITC conjugated
    Biotin CSB-PA004372LD01HU CA4 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.
  • 基因功能参考文献：
    1. CA4 is a novel tumour suppressor in Colorectal cancer through the inhibition of the Wnt signalling pathway by targeting the WTAP-WT1-TBL1 axis. CA4 methylation may serve as an independent biomarker for the recurrence of Colorectal cancer . PMID: 26071132
    2. Carbonic anhydrase 4 is lost in papillary thyroid carcinoma but not thyroid nodules PMID: 24880201
    3. Although CA IV has been shown to be active in mediating CO2 and HCO3 (-) transport in many important tissues like kidney and lung, and in isolated cells from brain and muscle, the gene for CA IV appears not to be essential. PMID: 24146379
    4. Coexpression of CAIV with MCT1 and MCT4 resulted in a significant increase in MCT transport activity. PMID: 24338019
    5. membrane-associated CA IV contributes robust catalytic activity intracellularly, and this activity participates in regulating H(+) dynamics in the cytosol PMID: 23297198
    6. Two SNPs of carbonic anhydrase IV, which are responsible for amino acid changes, were found. The frequencies of these SNPs were not significantly different between the caries-free group and the group with caries PMID: 22021688
    7. compared the subcellular localization and post-translational processing of wild-type (WT)- and retinitis pigmentosa 17 mutant-CAIV in three cell types PMID: 20626030
    8. The results suggest that the expression level of carbonic anhydrase IV may be important to maintain retina function in retinitis pigmentosa. PMID: 20450258
    9. renal injury inflicted by expression of human carbonic anhydrase IV folding mutants was markedly enhanced by haploinsufficiency of the endoplasmic reticulum cochaperone p58(IPK). PMID: 20308551
    10. binding by extracellular loop 4 of the human AE1 Cl-/HCO3- exchanger PMID: 11994299
    11. carbonic anhydrase IV binds EC4 of NBC1, and this interaction is essential for full NBC1 activity PMID: 14567693
    12. mutation was found to cosegregate with the retinitis pigmentosa RP17 phenotype in two large families PMID: 15090652
    13. CA inhibitors may have long-term adverse effects on vision. PMID: 15563508
    14. characterized the CA IV expressed in Capan-1 cells PMID: 17409381
    15. A novel mutation has been identified in CA4 that provides further evidence that impaired pH regulation may underlie photoreceptor degeneration in retinitis pigmentosa PMID: 17652713
    16. CAIV p.R14W sequence variant found in one of the patients with Bothnia dystrophy phenotype is benign polymorphism in population of northern Sweden. PMID: 18344446
    17. Apoptosis induced by the CA IV mutants could be prevented, at least partially, by treating the cells with dorzolamide, a CA inhibitor PMID: 19211803

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  • 相关疾病：
    Retinitis pigmentosa 17 (RP17)
  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Alpha-carbonic anhydrase family
  • 组织特异性：
    Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels.
  • 数据库链接：

    HGNC: 1375

    OMIM: 114760

    KEGG: hsa:762

    STRING: 9606.ENSP00000300900

    UniGene: Hs.89485