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BROX Antibody

  • 货号：
    CSB-PA730136LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate
      All lanes: BROX antibody at 3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 47, 43 kDa
      Observed band size: 47 kDa
    • IHC image of CSB-PA730136LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA730136LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) BROX Polyclonal antibody
  • Uniprot No.：
    Q5VW32
  • 基因名：
    BROX
  • 别名：
    BRO1 domain and CAAX motif containing antibody; BRO1 domain containing protein antibody; BRO1 domain- and CAAX motif-containing protein antibody; BRO1 domain-containing protein BROX antibody; BROFTI antibody; Brox antibody; BROX_HUMAN antibody; C1orf58 antibody; FLJ32421 antibody; hCG_25336 antibody; RP11-452F19.1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human BRO1 domain-containing protein BROX protein (288-409AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，BROX Antibody (CSB-PA730136LA01HU)，的标记方式是Non-conjugated。对于BROX Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA730136LB01HU BROX Antibody, HRP conjugated ELISA
    FITC CSB-PA730136LC01HU BROX Antibody, FITC conjugated
    Biotin CSB-PA730136LD01HU BROX Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 基因功能参考文献：
    1. SGK1.1 regulates gene transcription upon a different set of genes some of which participate in cell survival pathways (BAG-4) and others in intracellular vesicular traffic (Brox). PMID: 25849655
    2. structural analysis of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding PMID: 22162750
    3. Results revealed that Strep-Brox(WT) exhibited accumulation in the perinuclear area suggested physiological significance of farnesylation of Brox in its subcellular distribution and efficient interaction with CHMP4s in vivo. PMID: 18190528
  • 亚细胞定位：
    Membrane; Lipid-anchor.
  • 蛋白家族：
    BROX family
  • 数据库链接：

    HGNC: 26512

    KEGG: hsa:148362

    STRING: 9606.ENSP00000343742

    UniGene: Hs.534965