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BRF1 Antibody

  • 货号：
    CSB-PA149603
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from HeLa cells and Jurkat cells, using TF3B antibody.
    • Immunohistochemistry analysis of paraffin-embedded human brain tissue using TF3B antibody.
    • Immunofluorescence analysis of MCF-7cells, using TF3B antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) BRF1 Polyclonal antibody
  • Uniprot No.：
    Q92994
  • 基因名：
    BRF1
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Synthesized peptide derived from internal of Human TF3B.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
    IF 1:100-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
  • 基因功能参考文献：
    1. In an analysis of families with a history of colorectal cancer, we associated germline mutations in BRF1 with predisposition to colorectal cancer. Seven of the identified variants (1 detected in 2 families) affected BRF1 mRNA splicing, protein stability, or expression and/or function. PMID: 28912018
    2. Site-directed mutagenesis combined with kinase assays and specific phosphosite immunodetection identified Ser-54 (S54) and Ser-334 (S334) as PKA target amino acids in vitro and in vivo. Phosphomimetic mutation of the C-terminal S334 markedly increased TIS11b half-life and, unexpectedly, enhanced TIS11b activity on mRNA decay. PMID: 27708140
    3. Mutations in BRF1 cause severe short stature, remarkably delayed bone age, dysmorphic features, cerebellar hypoplasia and cognitive dysfunction inherited in an autosomal recessive pattern. PMID: 27748960
    4. Brf1 expression is increased in human HCC cases, which is correlated with shorter survival times. PMID: 26701855
    5. BRF1 mutations that reduce protein activity cause neurodevelopmental anomalies, suggesting that BRF1-mediated Pol III transcription is required for normal cerebellar and cognitive development. PMID: 25561519
    6. hnRNP F is a co-factor in a subset of tristetraprolin/BRF1/BRF2-mediated mRNA decay. PMID: 24978456
    7. these observations are in favor of a cell- and context-dependent regulation of Tis11b by hypoxia, which then contributes to modulation of angiogenesis. PMID: 21832157
    8. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression. PMID: 21106530
    9. results identify a human Pol III isoform and isoform-specific functions in the regulation of cell growth and transformation PMID: 20154270
    10. CK2 forms a stable complex with TFIIIB and activates RNA polymerase III transcription in human cells. PMID: 11997511
    11. human small nuclear RNA gene-specific transcription factor IIIB complex de novo on and off promoter PMID: 12016223
    12. BRF1 accelerated mRNA decay and antagonized the stabilizing effect of PI3-kinase, while mutation of the zinc fingers abolished both function and ARE-binding activity. This approach identifies BRF1 as an essential regulator of ARE-dependent mRNA decay. PMID: 12198173
    13. Data report that protein kinase B (PKB/Akt) stabilizes ARE transcripts by phosphorylating butyrate response factor (BRF1) at serine 92. PMID: 15538381
    14. These results suggest a direct role of an RNA polymerase III transcription factor in the targeting process. PMID: 16982688
    15. depletion of endogenous TTP and BRF-1 proteins, or overexpression of dominant-negative mutant TTP proteins, impairs the localization of reporter AU-rich element mRNAs PMID: 17369404
    16. Maf1 occupancy of Pol III genes is inversely correlated with that of the initiation factor TFIIIB (subunit Brf1) and Pol III. PMID: 17499043
    17. the hypo-phosphorylated Rb appeared to be largely sequestered into a complex with Brf1, which resulted in the blockage of Rb function to repress E2F1 transactivation PMID: 17877750
    18. Brf1 gene was identified in the genome-wide loss-of-function genetic screen as putative tumor suppressor located at 14q32.33. PMID: 17968325
    19. MK2-mediated inhibition of BRF1 requires phosphorylation at S54, S92, and S203. PMID: 18326031
    20. deregulation of Brf1 and Brf2 expression could be a key mechanism responsible for the observed deregulation of RNA pol III transcription in cancer cells PMID: 18700021

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  • 相关疾病：
    Cerebellofaciodental syndrome (CFDS)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIB family
  • 数据库链接：

    HGNC: 11551

    OMIM: 604902

    KEGG: hsa:2972

    STRING: 9606.ENSP00000448323

    UniGene: Hs.424484