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BRAP Antibody

  • 货号：
    CSB-PA773793LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse Brain tissue lysate, Mouse Heart tissue lysate, Rat Heart tissue lysate,
      All lanes: BRAP antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 68 kDa
      Observed band size: 68 kDa
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate
      All lanes: BRAP antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 68 kDa
      Observed band size: 68 kDa
    • IHC image of CSB-PA773793LA01HU diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
    • Immunofluorescence staining of Hela cell with CSB-PA773793LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) BRAP Polyclonal antibody
  • Uniprot No.：
    Q7Z569
  • 基因名：
  • 别名：
    BRAP antibody; RNF52BRCA1-associated protein antibody; EC 2.3.2.27 antibody; BRAP2 antibody; Impedes mitogenic signal propagation antibody; IMP antibody; RING finger protein 52 antibody; RING-type E3 ubiquitin transferase BRAP2 antibody; Renal carcinoma antigen NY-REN-63 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat, Mouse
  • 免疫原：
    Recombinant Human BRCA1-associated protein (13-124AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，BRAP Antibody (CSB-PA773793LA01HU)，的标记方式是Non-conjugated。对于BRAP Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA773793LB01HU BRAP Antibody, HRP conjugated ELISA
    FITC CSB-PA773793LC01HU BRAP Antibody, FITC conjugated
    Biotin CSB-PA773793LD01HU BRAP Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
  • 基因功能参考文献：
    1. Six polymorphisms (rs12229654 at 12q24.1, rs671 of ALDH2, rs11066015 of ACAD10, rs2074356 and rs11066280 of HECTD4, and rs3782886 of BRAP) were found to be associated with both systolic and diastolic blood pressure, with those at 12q24.1 or in ACAD10 or BRAP being novel determinants of blood pressure in Japanese. PMID: 28562329
    2. IMPACT data were consistent with increased risks of onset among BRCA1 and BRCA2 mutation carriers PMID: 27742670
    3. Expression of BRAP is increased in esophageal squamous cell carcinoma samples compared with non-tumor esophageal tissues; increased expression correlates with reduced patient survival time and promotes metastasis of xenograft tumors in mice. PMID: 28780075
    4. Together the results indicate for the first time that BRAP2 may play an important NRNI role in germ cells of the testis, with an additional, scaffold/structural role in mature spermatozoa. PMID: 25820252
    5. BRAP gene may confer vulnerability for schizophrenia in Han Chinese population. PMID: 24454952
    6. ectopic expression of BRAP2 inhibits nuclear localization of HMG20A and NuMA1, and prevents nuclear envelope accumulation of SYNE2. PMID: 23707952
    7. genetic association studies in population of Chinese Han young adults in Beijing: Data suggest that 2 SNPs in BRAP (rs11066001; rs3782886) are associated with decreased risk of metabolic syndrome in this population. PMID: 22965072
    8. BAP1 expression closely correlates with age, clinical stage, pathologic differentiation and histological type in colorectal cancer (CRC). BAP1 may serve as a novel prognostic biomarker for CRC. PMID: 23526420
    9. Brap2 regulates temporal control of NF-kappaB localization mediated by inflammatory response. PMID: 23554956
    10. The BRAP polymorphism may not play an important role in ischemic stroke in the Taiwanese population. PMID: 23356535
    11. The dominant effect of prolonged USP15 depletion upon signal amplitude is due to a decrease in CRAF levels while allowing for the possibility that USP15 may also function to dampen MAPK signaling through direct stabilization of BRAP. PMID: 23105109
    12. ANRIL on 9p21 and BRAP were both associated with ankle brachial index in a Taiwanese population. PMID: 22122968
    13. Several BRCA1 mutations were observed in Pakistani breast cancer patients with moderate family history. PMID: 22078348
    14. Data show that BRAP conferred a risk for carotid plaque and intima-medial thickness. PMID: 21670849
    15. BRAP gene is associated with the extent of coronary atherosclerosis and has a synergistic effect with diabetes on the occurrence of significant CAD in the Chinese population PMID: 22085839
    16. our approach, which is applicable to any set of interval scale traits that is heritable and exhibits evidence of phenotypic clustering, identified three new loci in or near APOC1, BRAP, and PLCG1, which were associated with multiple phenotype domains. PMID: 22022282
    17. Polymorphism of 270 A > G in BRAP is Associated with Lower Ankle-Brachial Index and peripheral artery disease. PMID: 21301165
    18. All results are consistent with BRAP2 being a novel, phosphorylation-regulated negative regulator of nuclear import, with potential as an antiviral agent. PMID: 20040518
    19. Data indicate that one SNP in BRAP showed(rs11066001) a significant association in allele frequency distribution with CAD in both the Japanese and Korean populations. PMID: 19713974
    20. modulates sensitivity of the MAP kinase cascade to stimulus-dependent activation by limiting functional assembly of the core enzymatic components through the inactivation of KSR PMID: 14724641
    21. Monocytic differentiation of the promyelomonocytic cell lines U937 and HL60 is associated with the upregulation of Brap2 expression concomitantly with the upregulation and cytoplasmic relocalization of p21. PMID: 15340083
    22. the capacity of IMP to inhibit signal propagation through Raf to MEK is a consequence of disrupting KSR1 homooligomerization and B-Raf/c-Raf hetero-oligomerization PMID: 18332145
    23. The most current evidence suggests that it may be more beneficial in those with BRCA mutations because tumors associated with these mutations are likely to be estrogen-receptor positive. PMID: 18380995
    24. p21 Ras/Imp regulate cytokine production and migration in CD4 T cells [Imp] PMID: 18577512
    25. Carriers of mutations in the genes BRCA1/2 may present a specific high risk group for PABC especially at younger ages. PMID: 19009954
    26. The s found that Brap2, which has intrinsic RING domain dependent E3 ligase activity, facilitates HsCdc14A Lys-63 linked ubiquitin modification, indicating that Brap2 may be the ubiquitin E3 Ligase of HsCdc14A. PMID: 19152073
    27. SNPs in BRAP associated with risk of myocardial infarction in Asian populations.BRAP knockdown in cultured coronary endothelial cells suppressed activation of NF-kappaB. PMID: 19198608

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  • 亚细胞定位：
    Cytoplasm.
  • 组织特异性：
    Expressed in breast epithelial cell lines.
  • 数据库链接：

    HGNC: 1099

    OMIM: 604986

    KEGG: hsa:8315

    STRING: 9606.ENSP00000403524

    UniGene: Hs.530940