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BCAT1 Antibody

  • 货号：
    CSB-PA005777
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of K562 cells using ECA39 Polyclonal Antibody.
  • 其他：

产品详情

  • Uniprot No.：
    P54687
  • 基因名：
    BCAT1
  • 别名：
    BCAT antibody; BCAT(c) antibody; Bcat1 antibody; BCAT1_HUMAN antibody; BCT1 antibody; Branched chain amino acid aminotransferase; cytosolic antibody; branched chain amino-acid transaminase 1; cytosolic antibody; branched chain aminotransferase 1 antibody; branched chain aminotransferase 1; cytosolic antibody; Branched-chain-amino-acid aminotransferase antibody; cytosolic antibody; DKFZp686E12175 antibody; EC 2.6.1.42 antibody; ECA39 antibody; MECA39 antibody; placental protein 18 antibody; PNAS 121 antibody; PP18 antibody; Protein ECA39 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from the Internal region of Human ECA39.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    ELISA 1:20000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
  • 基因功能参考文献：
    1. regulatory role in macrophage function PMID: 28699638
    2. Data suggest that both oncogenic mutations and cancer tissue-of-origin influence BCAA (branched-chain amino acid) metabolism in neoplastic tissue and neoplastic expression of cytosolic BCAT1 and mitochondrial BCAT2. (BCAT = branched-chain-amino-acid transaminase) [REVIEW] PMID: 29211698
    3. by limiting intracellular alphaKG, BCAT1 links branched-chain amino acid catabolism to HIF1alpha stability and regulation of the epigenomic landscape, mimicking the effects of IDH mutations; results suggest the BCAA-BCAT1-alphaKG pathway as a therapeutic target to compromise leukaemia stem-cell function in patients with IDH(WT)TET2(WT) AML PMID: 29144447
    4. BCAT1 plays a pathogenic role in hepatocellular carcinoma (HCC) by causing cell proliferation and chemoresistance. The MYC transcription factor is involved in regulating the transcriptional activity of BCAT1. BCAT1 expression has prognostic significance for the survival of patients with HCC. PMID: 27246112
    5. Phenotypic analyses using a lentiviral-mediated BCAT1 short hairpin RNA knockdown revealed that BCAT1 sustains proliferation in addition to migration and invasion and that its overexpression enhanced the capacity of antiestrogen-sensitive cells to grow in the presence of antiestrogens. PMID: 28319069
    6. The tumor suppressive role of miR-218 was mediated by negatively regulating branched-chain amino acid transaminase 1 (BCAT1) protein expression. Importantly, overexpression of BCAT1 decreased the chemosensitivity to cis-diaminedichloroplatinum treatment of PC3 and DU145 cells. PMID: 28052414
    7. It has been demonstrated that branched-chain amino acid (BCAA) catabolism is activated in human breast cancer, and abolishment of BCAA catabolism by knocking down BCAT1 inhibits breast cancer cell growth by repressing mTOR-mediated mitochondrial biogenesis and function. PMID: 28235484
    8. BCAT1 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    9. Mechanistic investigation revealed that BCAT1 might be an important regulator responsible for DOT1L-mediated sphere formation and cell migration in breast cancer cells PMID: 26783998
    10. BCAT1 overexpression is associated with advanced tumour status, and implies adverse clinical outcomes of urothelial carcinoma PMID: 26173071
    11. BCAT1 is strongly overexpressed in ovarian cancer. PMID: 26372729
    12. Focal deletions of the BCAT1 were associated with B-cell precursor acute lymphoblastic leukemia. PMID: 25261097
    13. levels of branched-chain aminotransferase-1 (BCAT1) transcripts are significantly decreased on the polysomes of both RPS19 and RPL11 cells and that translation of BCAT1 protein is especially impaired in cells with small RP gene mutations PMID: 24463277
    14. Over-expression of BCAT1, a c-Myc target gene, induces cell proliferation, migration and invasion in nasopharyngeal carcinoma. PMID: 23758864
    15. A central role for BCAT1 in glioma pathogenesis, making BCAT1 and branched-chain amino acids metabolism attractive targets for the development of targeted therapeutic approaches to treat patients with glioblastoma. PMID: 23793099
    16. The mitochondrial isoform human brain BCAT 2 is largely confined to vascular endothelial cells, whereas the cytosolic human brain BCAT 1 is restricted to neurons. PMID: 23043456
    17. BCATc (cytosolic) has an overall redox potential that is 30 mV lower than BCATm (mitochondrial). Furthermore, the CXXC motif of BCATc was estimated to be 80 mV lower, suggesting that BCATm is more oxidizing in nature. PMID: 22107788
    18. Our results suggest that ECA39 is a dominant predictive factor for distant metastasis in patients with advanced colorectal cancer (CRC). PMID: 17007058
    19. The BCAT1 identified in the amplified 12p11-p12 region may play a certain role in nasopharyngeal carcinoma development. PMID: 18074675
    20. hBCATc has redox mediated associations with several neuronal proteins involved in G-protein cell signaling, indicating a novel role for hBCATc in cellular redox control PMID: 18419134
    21. The effect of nitric oxide modification on the functionality of human mitochondrial and cytosolic branched-chain aminotransferases (hBCATm and hBCATc, respectively) was investigated. PMID: 19119849

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-IV pyridoxal-phosphate-dependent aminotransferase family
  • 组织特异性：
    During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
  • 数据库链接：

    HGNC: 976

    OMIM: 113520

    KEGG: hsa:586

    STRING: 9606.ENSP00000443459

    UniGene: Hs.438993