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ASIC2 Antibody

  • 货号：
    CSB-PA613683LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA613683LA01HU diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA613683LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA613683LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ASIC2 Polyclonal antibody
  • Uniprot No.：
    Q16515
  • 基因名：
    ASIC2
  • 别名：
    ACCN antibody; ACCN1 antibody; ACCN1_HUMAN antibody; Acid sensing ion channel 2 antibody; Acid-sensing ion channel 2 antibody; Amiloride sensitive brain sodium channel antibody; Amiloride sensitive cation channel 1 neuronal (degenerin) antibody; Amiloride sensitive cation channel 1 neuronal antibody; Amiloride-sensitive brain sodium channel antibody; Amiloride-sensitive cation channel 1; neuronal antibody; Amiloride-sensitive cation channel neuronal 1 antibody; ASIC2 antibody; ASIC2a antibody; BNaC1 antibody; BNC1 antibody; Brain sodium channel 1 antibody; Degenerin antibody; hBNaC1 antibody; Mammalian degenerin homolog antibody; MDEG antibody; Neuronal amiloride sensitive cation channel 1 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Acid-sensing ion channel 2 protein (197-345AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ASIC2 Antibody (CSB-PA613683LA01HU)，的标记方式是Non-conjugated。对于ASIC2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA613683LB01HU ASIC2 Antibody, HRP conjugated ELISA
    FITC CSB-PA613683LC01HU ASIC2 Antibody, FITC conjugated
    Biotin CSB-PA613683LD01HU ASIC2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.
  • 基因功能参考文献：
    1. Study shows that ASIC2 promotes metastasis of colorectal cancer (CRC) cells by activating the calcineurin/NFAT1 pathway under acidosis and high expression of ASIC2 predicts poor outcomes of patients with CRC. PMID: 28927426
    2. The s finally confirmed that the ASIC2b isoform is delivered to the cell surface from the endoplasmic reticulum by forming heteromers with ASIC2a isoform, and that the N-terminal region of ASIC2a is additionally required for the ASIC2a-dependent membrane targeting of ASIC2b. PMID: 27477936
    3. The ASIC2 are involved in fear behaviours, learning and memory functions, and pain sensation. PMID: 27278329
    4. The results show that acid-sensing ion channel 1, acid-sensing ion channel 2, and acid-sensing ion channel 3 are expressed in A549 cells at the messenger RNA and protein levels, and acid-sensing ion channel-like currents were elicited by extracellular acid stimuli. PMID: 28618956
    5. ASICs may enhance susceptibility to epileptogenesis in the piriform cortex. PMID: 25744567
    6. results demonstrate the occurrence of ASIC2 and TrkB in the human intervertebral disc (IVD), and the increased expression of both in pathological IVD suggest their involvement in IVD degeneration PMID: 26617738
    7. distribution of ASIC2 in the human cutaneous mechanosensory system and suggest the involvement of ASIC2 in mechanosensation PMID: 25224144
    8. This study suggests that ASIC2 may play a role as mediators of inflammatory pain and be involved in the pathogenesis of frozen shoulder. PMID: 26033064
    9. Combinatorial analysis suggests a model of random mixing of ASIC1a and ASIC2a subunits to yield both 2:1 and 1:2 ASIC1a:ASIC2a heteromers together with ASIC1a and ASIC2a homomers. PMID: 24847067
    10. The results showed that there was a significant increase in the mean relative optical density of ASIC2 and ASIC3 but not ASIC1a in the lining epithelium and glandular tubes of gastric mucosa in patients with Henoch-Schonlein purpura. PMID: 22157923
    11. ACCN1 might be one of numerous susceptibility genes for panic disorder. PMID: 21811305
    12. these results indicate that ACCN1 gene is a potential candidate for response to lithium treatment that would serve as a genetic marker of lithium efficacy for bipolar disorder patients. PMID: 21961650
    13. These results demonstrate for the first time the differential distribution of ASIC1 and ASIC2 in human rapidly adapting low-threshold mechanoreceptors, and suggest specific roles of both proteins in mechanotransduction. PMID: 20306292
    14. Application of these methods allowed us to find a significant association between MS and the SNP rs28936 located in the 3' UTR segment of ACCN1 with p = 0.0004 (p = 0.002, after adjusting for multiple testing) PMID: 17534430
    15. the ASIC2a subtype consists of intracellular amino and carboxyl termini and two transmembrane domains connected by a large extracellular loop PMID: 15504740
    16. ASIC2 surface expression inhibits the amiloride-sensitive current and migration of glioma cells PMID: 16704974
    17. AKAP150 and the protein phosphatase calcineurin are binding proteins to ASIC2a, and calcineurin regulates ASIC1a and ASIC2a activity PMID: 17548344
    18. This work provides a mechanism explaining how defects in parkin-mediated PICK1 monoubiquitination could enhance ASIC activity and thereby promote neurodegeneration in Parkinson's disease. PMID: 17553932
    19. Two PKC consensus sites on human acid-sensing ion channel 1b differentially regulate its function. PMID: 19091960

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family, ASIC2 subfamily
  • 组织特异性：
    Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.
  • 数据库链接：

    HGNC: 99

    OMIM: 601784

    KEGG: hsa:40

    UniGene: Hs.368417