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ARHGAP18 Antibody

  • 货号：
    CSB-PA850791LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Rat lung tissue, Rat kidney tissue, Mouse heart tissue
      All lanes: ARHGAP18 antibody at 4.5µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 75, 71 kDa
      Observed band size: 75 kDa
    • IHC image of CSB-PA850791LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA850791LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ARHGAP18 Polyclonal antibody
  • Uniprot No.：
    Q8N392
  • 基因名：
    ARHGAP18
  • 别名：
    4833419J07Rik antibody; AI314906 antibody; AI843501 antibody; Arhgap18 antibody; bA307O14.2 antibody; FLJ25728 antibody; MacGAP antibody; MGC126757 antibody; MGC138145 antibody; OTTHUMP00000017190 antibody; OTTMUSP00000028606 antibody; RHG18_HUMAN antibody; Rho GTPase-activating protein 18 antibody; Rho-type GTPase-activating protein 18 antibody; SENEX antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat, Mouse
  • 免疫原：
    Recombinant Human Rho GTPase-activating protein 18 protein (389-577AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ARHGAP18 Antibody (CSB-PA850791LA01HU)，的标记方式是Non-conjugated。对于ARHGAP18 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA850791LB01HU ARHGAP18 Antibody, HRP conjugated ELISA
    FITC CSB-PA850791LC01HU ARHGAP18 Antibody, FITC conjugated
    Biotin CSB-PA850791LD01HU ARHGAP18 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Rho GTPase activating protein that suppresses F-actin polymerization by inhibiting Rho. Rho GTPase activating proteins act by converting Rho-type GTPases to an inactive GDP-bound state. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts downstream of YAP1 and inhibits actin polymerization, which in turn reduces nuclear localization of YAP1. Regulates cell shape, spreading, and migration.
  • 基因功能参考文献：
    1. Our data suggest that ARHGAP18, which was located by the SNP rs11759328 via positive selection, plays a potential role in regulating HbF expression in beta-thalassaemia and may be a promising therapeutic target PMID: 28983712
    2. these results define opposing roles for oncogenic ARHGAP18 and tumor suppressive miR-200b in determining triple-negative breast cancer cell migration and metastatic prowess PMID: 28619708
    3. data suggest the ARHGAP18 may confer vulnerability to SZ in the Chinese Han population, providing additional evidence for the involvement of neurodevelopmental dysfunction in the pathogenesis of schizophrenia PMID: 28384650
    4. In endothelial cells, ARHGAP18 may act as a significant regulator of vascular homeostasis. PMID: 28251925
    5. Our results show that miR-181a is down-regulated in glioblastoma multiforme (GBM) patients. The three target genes, ANGPT2, ARHGAP18 and LAMC1, are negatively correlated with the expression of miR-181a. Moreover, high expression of ANGPT2 or LAMC1 together with large size of GBM is correlated with a shorter median overall survival PMID: 27176932
    6. Results describe ARHGAP18 as a novel negative regulator of sprouting by acting dualistically to limit tip cell formation and to maintain junctional integrity. PMID: 25425145
    7. The results define ARHGAP18 as one of the crucial factors for the regulation of RhoA for the control of cell shape, spreading, and migration. PMID: 21865595
    8. identification of a novel gene, SENEX, that regulates stress induced premature senescence pathways in endothelial cells involving p16(INK4a) and retinoblastoma protein activation PMID: 20664062
    9. This study use Functional MRI and Genome Wide Association Analysis indentic novel gene(ARHGAP18) associated with schizophrenia. PMID: 19065146

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    HGNC: 21035

    OMIM: 613351

    KEGG: hsa:93663

    STRING: 9606.ENSP00000275189

    UniGene: Hs.486458