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ARFGAP1 Antibody

  • 货号：
    CSB-PA843284ESR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: ADP-ribosylation factor GTPase-activating protein 1 antibody at 5μg/ml
      Lane 1: PC-3 whole cell lysate
      Lane 2: Hela whole cell lysate
      Lane 3: Mouse brain tissue
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 45, 46, 32, 40 kDa
      Observed band size: 45 kDa
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA843284ESR2HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ARFGAP1 Polyclonal antibody
  • Uniprot No.：
    Q8N6T3
  • 基因名：
  • 别名：
    ADP ribosylation factor 1 GTPase activating protein antibody; ADP ribosylation factor GTPase activating protein 1 antibody; ADP-ribosylation factor 1 GTPase-activating protein antibody; ADP-ribosylation factor GTPase-activating protein 1 antibody; ARF GAP 1 antibody; ARF1 directed GTPase activating protein antibody; ARF1 GAP antibody; ARF1-directed GTPase-activating protein antibody; ARF1GAP antibody; ARFG1_HUMAN antibody; Arfgap1 antibody; GAP protein antibody; HRIHFB2281 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human ADP-ribosylation factor GTPase-activating protein 1 protein (1-220AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
  • 基因功能参考文献：
    1. ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes. PMID: 25397679
    2. ARFGAP1 is a host factor for Hepatitis C Virus RNA replication, and is hijacked by HCV NS5A to remove COPI cargo Sac1 from the site of HCV replication to maintain high levels of PI4P. PMID: 24623438
    3. ArfGAP1 is identified as the first GTPase activating protein (GAP) for leucine-rich repeat kinase 2 (LRRK2). PMID: 22423108
    4. role of ARFGAP1 in AP-2-regulated endocytosis has mechanistic parallels with its roles in COPI transport, as both its GAP activity and coat function contribute to promoting AP-2 transport PMID: 21499258
    5. The small GTPases of the Ras superfamily mediate numerous biological processes through their ability to cycle between an inactive GDP-bound and an active GTP-bound form. PMID: 15246431
    6. ArfGAP1 interacts with the Golgi through multiple hydrophobic motifs and that alternative modes of interaction may exist in tissue-specific ArfGAP1 isoforms. PMID: 16316994
    7. SMAP2 functions in the retrograde, early endosome-to-trans golig network pathway in a clathrin- and AP-1-dependent manner. PMID: 16571680
    8. coatomer is an allosteric regulator of Arf GAP1 PMID: 18541532
    9. Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking PMID: 19015319
    10. catalytic amounts of Arf1GAP1 significantly reduce the yield of purified COPI vesicles PMID: 19055691
    11. ArfGAP1, ArfGAP2, and ArfGAP3 have overlapping roles in regulating COPI function in Golgi-to-ER retrograde transport. PMID: 19299515

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  • 亚细胞定位：
    Cytoplasm. Golgi apparatus.
  • 数据库链接：

    HGNC: 15852

    OMIM: 608377

    KEGG: hsa:55738

    STRING: 9606.ENSP00000314615

    UniGene: Hs.25584