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AMOT Antibody

  • 货号：
    CSB-PA001677ESR2HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: Angiomotin antibody at 2μg/ml + 293T whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 119, 73 kDa
      Observed band size: 119 kDa
    • Western Blot
      Positive WB detected in: A549 whole cell lysate
      All lanes: AMOT antibody at 1.21μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 119 kDa
      Observed band size: 130 kDa
    • IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) AMOT Polyclonal antibody
  • Uniprot No.：
    Q4VCS5
  • 基因名：
  • 别名：
    AMOT antibody; AMOT_HUMAN antibody; Angiomotin antibody; Angiomotin p130 isoform antibody; KIAA1071 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Angiomotin protein (1-300AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
  • 基因功能参考文献：
    1. Angiomotin promotes prostate cancer cell proliferation by signaling through the Hippo-YAP pathway. PMID: 28052036
    2. The s propose that phosphorylation of Amot(S176) is a critical post-translational modification that suppresses YAP's ability to promote cell proliferation and tumorigenesis by altering the subcellular localization of an essential YAP co-factor. PMID: 28464980
    3. Data indicate that Amot is crucial for the maintenance of nuclear YAP to promote renal epithelial and RCC proliferation. PMID: 26848622
    4. Decreased AMOT-p130 expression coupled with high nuclear YAP1 expression resulted in shorter overall survival and disease-free survival in patients with advanced gastric cancer. PMID: 28885730
    5. Study focused on the methylation profile of the AMOT promoter CpG island during development, comparing it in circulating cord blood endothelial progenitor cells (ECFC) of cord blood from term versus preterm newborns. Findings highlight importance of pro-angiogenic AMOT gene methylation in ECFC, suggesting that epigenetic mechanisms may control the regulation of angiogenesis during development. PMID: 29036193
    6. AMOT may function as an oncogene in the progression of colon cancer by activating the YAP-ERK/PI3K-AKT signaling pathway. PMID: 27779692
    7. angiomotin and Merlin respectively interface cortical actin filaments and core kinases in Hippo signaling PMID: 26045165
    8. Study shows miR-205 significantly downregulated and directly target the 3'-UTR of AMOT in breast cancer. In vitro, miR-205 regulates the proliferation and invasion of breast cancer cells through suppression of AMOT expression. PMID: 26239614
    9. Amot was highly expressed in breast cancer tissues and was important in the promotion of breast cancer cell proliferation and invasion. Amot knockdown in MCF-7 cells decreased the expression of YAP, YAP/TAZ and LATS1. PMID: 25647626
    10. experiments indicate that AMOT and other motin family members function together with NEDD4L to help complete immature virion assembly prior to ESCRT-mediated virus budding PMID: 25633977
    11. AMOT is a crucial suppressor of lung cancer metastasis and highlight its critical role as a tumor suppressor and its potential as a prognostic biomarker and therapeutic target for lung cancer. PMID: 25381822
    12. expression is upregulated in sinonasal inverted papilloma PMID: 24532565
    13. angiomotin proteins connect F-actin architecture to YAP regulation. PMID: 24648494
    14. function of Angiomotins and other members of the Motin protein family PMID: 24548561
    15. Scaffold proteins angiomotin (Amot) and angiomotin-related AmotL1 and AmotL2 were recently identified as negative regulators of YAP and TAZ by preventing their nuclear translocation. PMID: 24003252
    16. Within the nucleus, Amot-p130 was associated with the transcriptional complex containing Yap and Teads (TEA domain family members) and contributed to the regulation of a subset of Yap target genes, many of which are associated with tumorigenesis. PMID: 24003254
    17. These results collectively suggest that the Hippo pathway negatively regulates the actin-binding activity of Amot family members through direct phosphorylation. PMID: 24225952
    18. Thus AMOT is a direct substrate of Lats1/2 mediating functions of the Hippo pathway in endothelial cell migration and angiogenesis. PMID: 24106267
    19. Data indicate that the phosphorylation of Amot130 by LATS1/2 is found to be a key feature that enables it to inhibit Yes-associated protein (YAP) dependent signaling and cell growth. PMID: 24101513
    20. High levels of Amot transcripts were associated with poor differentiation, venous invasion and decreased survival in patients with clear cell renal cell carcinoma. PMID: 23588948
    21. Soluble melanoma cell adhesion molecule (sMCAM/sCD146) promotes angiogenic effects on endothelial progenitor cells through angiomotin PMID: 23389031
    22. Three L/P-PXY motifs of AMOT/p130 and the WW domains of Nedd4 mediate the interaction of Nedd4, Nedd4-2 and Itch. PMID: 22385262
    23. Data show that Amot expression is required for proliferation of breast cancer cells in specific microenvironmental contexts that require ERK1/2 signaling. PMID: 21285250
    24. ADepletion of Angiomotin in Nf2(-/-) Schwann cells attenuates the Ras-MAPK signaling pathway, impedes cellular proliferation in vitro and tumorigenesis in vivo PMID: 21481793
    25. novel mechanism to restrict the activity of TAZ and YAP through physical interaction with Amot and AmotL1 PMID: 21224387
    26. a novel lipid binding domain within Amot is shown to selectively bind with high affinity to membranes containing monophosphorylated phosphatidylinositols and cholesterol PMID: 20080965
    27. PDZ binding motif of angiomotin plays a critical role in regulating the responsiveness of endothelial cells to chemotactic cues PMID: 12902404
    28. angiomotin, in addition to controlling cell motility, may play a role in the assembly of endothelial cell-cell junctions PMID: 16043488
    29. p80- and p130-angiomotin play coordinating roles in vascular tube formation by affecting cell migration and cell shape, respectively. PMID: 16640563
    30. Results show that DNA vaccination targeting angiomotin may be used to mimic the effect of angiostatin. PMID: 16754857
    31. the directional migration of capillaries in the embryo is governed by the Amot:Patj/Mupp1:Syx signaling that controls local GTPase activity PMID: 18824598
    32. The angiomotin-like 1 is involved in actin-cytoskeleton-based processes, in part, via its interaction with a p80-angiomotin-containing complex and the actin cytoskeleton PMID: 19565639
    33. Amot and AmotL1 have similar effects on endothelial migration and tight junction formation in vitro. In vivo Amot appears to control the cell polarity and AmotL1 affects the stability of cell-cell junctions. PMID: 19590046

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  • 亚细胞定位：
    Cell junction, tight junction. Note=Localized on the cell surface. May act as a transmembrane protein.
  • 蛋白家族：
    Angiomotin family
  • 组织特异性：
    Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
  • 数据库链接：

    HGNC: 17810

    OMIM: 300410

    KEGG: hsa:154796

    STRING: 9606.ENSP00000361027

    UniGene: Hs.528051