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AMN Antibody

  • 货号：
    CSB-PA880128LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA880128LA01HU diluted at 1:600 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA880128LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) AMN Polyclonal antibody
  • Uniprot No.：
    Q9BXJ7
  • 基因名：
    AMN
  • 别名：
    5033428N14Rik antibody; amn antibody; Amnion associated transmembrane protein antibody; Amnionless antibody; Amnionless homolog antibody; AMNLS_HUMAN antibody; AV002116 antibody; PRO1028 antibody; Protein amnionless antibody; Type I transmembrane protein antibody; UNQ513/PRO1028 antibody; Visceral endoderm specific type 1 transmembrane protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein amnionless protein (25-163AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，AMN Antibody (CSB-PA880128LA01HU)，的标记方式是Non-conjugated。对于AMN Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA880128LB01HU AMN Antibody, HRP conjugated ELISA
    FITC CSB-PA880128LC01HU AMN Antibody, FITC conjugated
    Biotin CSB-PA880128LD01HU AMN Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:500-1:1000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Membrane-bound component of the endocytic receptor formed by AMN and CUBN. Required for normal CUBN glycosylation and trafficking to the cell surface. The complex formed by AMN and CUBN is required for efficient absorption of vitamin B12. Required for normal CUBN-mediated protein transport in the kidney (Probable).
  • 基因功能参考文献：
    1. Study shows that cubilin mutations (novel and some previously reported) and all previously reported amnionless missense mutations resulted in endoplasmic reticulum retention and completely inhibited amnionless-dependent plasma membrane expression of cubilin. PMID: 29402915
    2. heterozygous mutations in AMN in a family from the United Kingdom with clinical features of Imerslund-Grasbeck Syndrome PMID: 26040326
    3. Our genetic screening of 154 families of patients with inherited cobalamin malabsorption revealed population-specific mutations, mutational hotspots, and functionally distinct regions in the three causal genes: CUBN, AMN, and GIF. PMID: 22929189
    4. We present evidence that this founder mutation causes over 50% of the Imerslund-Grasbeck syndrome (IGS) cases among Arabic, Turkish, and Sephardic Jewish families, and the mutation is as old as human civvlization. PMID: 22078000
    5. amnionless is essential for the correct luminal expression of cubilin in humans. PMID: 21750092
    6. homozygous mutations affecting exons 1-4 of human AMN lead to megaloblastic anemia 1 PMID: 12590260
    7. cubilin and amnionless are subunits of a novel cubilin/amnionless (cubam) complex PMID: 14576052
    8. Recurrent spontaneous abortions may be caused by mutations in the Amnionless gene. PMID: 16403802
    9. This review summarizes recent data on the biological function of amnionless and focuses on its implication in embryonic nutrition and central nervous system malformations. PMID: 17979745

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  • 相关疾病：
    Recessive hereditary megaloblastic anemia 1 (RH-MGA1)
  • 亚细胞定位：
    [Isoform 1]: Apical cell membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Endosome membrane. Membrane, coated pit.; [Soluble protein amnionless]: Secreted.
  • 组织特异性：
    Detected in proximal tubules in the kidney cortex (at protein level). Long isoforms are highly expressed in small intestine, colon and kidney (renal proximal tubule epithelial cells). Shorter isoforms are detected at lower levels in testis, thymus and per
  • 数据库链接：

    HGNC: 14604

    OMIM: 261100

    KEGG: hsa:81693

    STRING: 9606.ENSP00000299155

    UniGene: Hs.534494