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ALOX15B Antibody

  • 货号：
    CSB-PA550481
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA550481(ALOX15B Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    O15296
  • 基因名：
    ALOX15B
  • 别名：
    15 Lipoxygenase 2 antibody; 15 LO 2 antibody; 15 LOX 2 antibody; 15-lipoxygenase 2 antibody; 15-LOX-2 antibody; 15-LOX-B antibody; Alox15b antibody; Arachidonate 15 lipoxygenase second type antibody; Arachidonate 15 lipoxygenase type II antibody; Arachidonate 15-lipoxygenase B antibody; Arachidonate 15-lipoxygenase type II antibody; LX15B_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Fusion protein of Human ALOX15B
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    IHC 1:15-1:50
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist. Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates. Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively downregulate the immune response and have anti-aggregation properties with platelets. In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids. Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.; Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE.
  • 基因功能参考文献：
    1. Ca(2+) binding induces major structural changes in the 15-lipoxygenase-2 PLAT domain. PMID: 28809482
    2. 15-LOX-2 is distributed at the plasma membrane when cells are stimulated by the addition Ca(2+) ionophore and that cellular localization is dependent upon the presence of a putative membrane insertion loop. PMID: 27435673
    3. these results demonstrate the strict regiospecificity of h15-LOX-2 that circumscribes its role in transcellular synthesis. PMID: 27145229
    4. This study combined molecular dynamics simulations, QM/MM calculations, and umbrella sampling free energy simulations to study the hydrogen atom abstraction from arachidonic acid catalyzed by the human enzyme 15-lipoxygenase-2. PMID: 26918937
    5. The ALOX15B gene may be associated with coronary artery disease. PMID: 24373925
    6. Hpoxia-induced pulmonary vascular remodeling is associated with increased levels of 15-LO-2. PMID: 25895668
    7. ALOX15 rs11568070 polymorphisms did not discriminate for the disease or its severity. PMID: 24975552
    8. 15-LOX2 expression inhibits Myc-induced prostate cancer development, such that in the 3-month- and 6-month-old double transgenic mice, there is a significant reduction in prostate intraneoplasia. PMID: 24732589
    9. activated ALOX15B in macrophages may play a role in the induction of atherothrombotic events by increasing platelet aggregation and thrombin generation PMID: 24533104
    10. Positive feedback-loop of TERT and 15-lipoxygenase-2 promotes pulmonary hypertension. PMID: 24376652
    11. Presented is the crystal structure of 15-LOX-2 in complex with an inhibitor that appears to bind as a substrate mimic. 15-LOX-2 contains a long loop, composed of hydrophobic amino acids, which projects from the amino-terminal membrane-binding domain. PMID: 24497644
    12. ALOX15B is the mainly expressed 12/15-lipoxygenase in human macrophages and that its expression is induced by IL-4, LPS and hypoxia. PMID: 22980500
    13. The C2-domain is not essential for catalytic activity and does hardly impact reaction specificity. PMID: 21951814
    14. Tumor-associated macrophages isolated from RCC tumors had a high 15-LOX2 expression and secreted substantial amounts of 15(S)-hydroxyeicosatetraenoic acid. PMID: 21900394
    15. In summary, we observed associations between high ALOX15B expression in carotid lesions and a history of cerebrovascular symptoms. PMID: 21316676
    16. Results from this study suggest a 125-bp region (-157 to -33) is critical for the 15-LOX-2 promoter activity in prostate epithelial cells and cancer cells PMID: 20428779
    17. a negative cell cycle regulator in normal prostate epithelial cells, but not present in prostatic neoplasms PMID: 11839751
    18. 15-LOX-2 expression is lost in esophageal cancers and the induction of 15-LOX-2 can inhibit cancer cell proliferation PMID: 12659684
    19. 15-LOX2 and its splice variants suppress prostate tumor development PMID: 12704195
    20. Sp1 and Sp3 proteins play a physiologically important role in positively and negatively regulating the 15-LOX2 gene expression, respectively. PMID: 15247906
    21. feedback mechanisms may contribute to the loss of 15-LOX-2 pathway components, which coincide with an increase in PPAR-gamma in many epithelial cancers PMID: 15799828
    22. Crosstalk mechanisms exist between the 15-LOX-2 gene and peroxisome proliferator-activated receptor gamma (PPARgamma) to counterbalance expression and help explain the inverse relationship of these genes in normal prostate versus prostate cancer cells. PMID: 16682954
    23. Loss of heterozygosity on 17p13 and down-regulation of ALOX15B can be used to discriminate adrenal cortex neoplasms from adrenocortical adenoma. PMID: 18156936
    24. 13-(S)-hydroxyoctadecadienoic acid (13-HODE) is docked to solvent-exposed histidines of a 15-hLOX-2 homology model and found to bind well with histidine627, suggesting a potential location for the allosteric site. PMID: 19645454
    25. Expression of 8S-LOX and 15S-LOX-2 suppresses CRD-BP/IMP-1 expression, resulting in inhibition of human prostate carcinoma PC-3 cell proliferation. PMID: 19661680

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  • 亚细胞定位：
    [Isoform A]: Nucleus.; Cytoplasm, cytosol. Cell membrane. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Cell junction, adherens junction. Cell junction, focal adhesion.
  • 蛋白家族：
    Lipoxygenase family
  • 组织特异性：
    Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea.
  • 数据库链接：

    HGNC: 434

    OMIM: 603697

    KEGG: hsa:247

    STRING: 9606.ENSP00000369530

    UniGene: Hs.111256