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  4. AKAP12 Antibody

AKAP12 Antibody

  • 货号：
    CSB-PA610964
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA610964(AKAP12 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
    • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA610964(AKAP12 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    Q02952
  • 基因名：
    AKAP12
  • 别名：
    A kinase (PRKA) anchor protein (gravin) 12 antibody; A kinase Anchor Protein 12 antibody; A kinase anchor protein 250kDa antibody; A-kinase anchor protein 12 antibody; A-kinase anchor protein 250 kDa antibody; AKA12_HUMAN antibody; AKAP 12 antibody; AKAP 250 antibody; AKAP-12 antibody; Akap12 antibody; AKAP250 antibody; DKFZp686M0430 antibody; DKFZp686O0331 antibody; FLJ20945 antibody; FLJ97621 antibody; Gercelin antibody; Gravin antibody; Kinase scaffold protein gravin antibody; Myasthenia gravis autoantigen antibody; Myasthenia gravis autoantigen gravin antibody; OTTHUMP00000017426 antibody; OTTHUMP00000017427 antibody; Src-Suppressed C Kinase Substrate antibody; Srcs5 antibody; SSeCKS antibody; Tsga12 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthetic peptide of Human AKAP12
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:3000-1:10000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
  • 基因功能参考文献：
    1. Low AKAP12 expression is associated with high-grade meningiomas. PMID: 29391485
    2. There is a positive association between the expression of AKAP12 and Semaphorin 3F in prostate cancer, suggesting that the activation of Semaphorin 3F by AKAP12 may be involved in prostate cancer progression and metastasis. PMID: 28698137
    3. A critical role for AKAP12 as an UV-inducible scaffold for PKA-mediated ATR phosphorylation. PMID: 27683220
    4. the AKAP12a promoter shows high DNA methylation levels in juvenile myelomonocytic leukemia, which go along with reduced AKAP12a mRNA expression levels PMID: 26891149
    5. MiR-103 regulates hepatocellular carcinoma growth by targeting AKAP12 PMID: 26646106
    6. results suggest that the association between the 6q25.1 locus and breast cancer risk may be mediated through single nucleotide polymorphisms that regulate expressions of the AKAP12 gene. PMID: 26645718
    7. The s propose that Gravin-mediated recruitment of Aurora A and Plk1 to the mother (oldest) spindle pole contributes to the fidelity of symmetric cell division. PMID: 26406118
    8. The SSeCKS, a major Lipopolysaccharide (LPS) response protein, has been involved in regulating astrocyte activation via production of proinflammatory factor in CNS inflammation. PMID: 26428665
    9. Elevated expression of AKAP12 is characteristic of paclitaxel-resistant high-grade serous ovarian cancer cells, and a prognostic and predictive marker for poor overall survival in SOC patients. PMID: 25748058
    10. The enrichment of activated Cdc42 in SSeCKS-null leading edge filopodia correlated with recruitment of the Cdc42-specific guanine nucleotide exchange factor, Frabin. PMID: 25356636
    11. AKAP12 scaffolding protein mediates meningeal reconstruction after central nervous system injury PMID: 25229625
    12. PKA compartmentalization via AKAP220 and AKAP12 contributes to endothelial barrier regulation. PMID: 25188285
    13. This study showed that AKAP12,CAMK2D and a molecular pathway(cyclic amp)association to outcome of depressive during citalopram treatment. PMID: 24986638
    14. AKAP12 variant 2 hypoxic induction shifts PKA-mediated protein phosphorylation to enhance migration and metastasis of melanoma cells PMID: 25792458
    15. AKAP12 expression was positive in 82.2% of tumors in a cohort with colorectal adenocarcinoma. AKAP12 expression was unrelated to p53 or Bcl-2 expression. PMID: 24870731
    16. Receptor-mediated Ca2+ and PKC signaling triggers the loss of cortical PKA compartmentalization through the redistribution of gravin. PMID: 23838009
    17. High AKAP12 expression is associated with colorectal cancer. PMID: 24065476
    18. AKAP12 is differentially expressed in human astrocytomas showing high expression in pilocytic but low expression in diffuse astrocytomas of all WHO-grades. further, epigenetic mechanisms are involved in silencing AKAP12 in diffuse astrocytomas PMID: 24042196
    19. Gravin gene expression is decreased in acute myeloid leukemia and correlates with poor prognosis. PMID: 23543478
    20. Data suggest a model in which SSeCKS suppresses oncogenic motility by sequestering Src to caveolin-rich lipid rafts, thereby disengaging Src from FAK-associated adhesion and signaling complexes. PMID: 22710722
    21. demonstrated for the first time that AKAP12 tumor/angiogenesis suppressor gene is an epigenetic target of HDAC7 PMID: 22584896
    22. It defines a role for Gravin as a temporal organizer of phosphorylation-dependent protein-protein interactions during mitosis. PMID: 23063527
    23. The results demonstrate that AKAP12 may play an important role in tumor growth suppression and the survival of human colorectal cancer. PMID: 21918680
    24. PKC-mediated remodeling of the actin cytoskeleton is likely regulated by the ability of SSeCKS to control PKC signaling and activity through a direct scaffolding function PMID: 21903576
    25. AKAP12 is involved in the regulation of endothelial cell migration through the inhibitory regulation of MMP-9 expression in tumor cells. PMID: 21461577
    26. AKAP12 promoter methylation is a frequent event in human prostate cancer. PMID: 21310466
    27. SERPINB5 and AKAP12 may have a role in increased metastasis in pancreatic ductal adenocarcinoma PMID: 20939879
    28. Data show that AKAP12 methylation represents a potential molecular biomarker for predicting the malignancy of colorectal cancer. PMID: 20364105
    29. AKAP12alpha promoter methylation is associated with lung cancer. PMID: 21115911
    30. In addition to genetic alterations, epigenetic mechanisms are responsible for the reduction of the tumor suppressor gene AKAP12 in human hepatocarcinogenesis. PMID: 20979053
    31. Findings suggest that SSeCKS suppresses metastatic motility by disengaging activated Src and then inhibiting the PKC-Raf/MEK/ERK pathways controlling matrix metalloproteinase-2 expression and podosome formation. PMID: 20018890
    32. gravin provides a dynamic platform for the localization for kinases during neuronal development PMID: 12857743
    33. AKAP250 acts as a scaffold that binds protein kinase A (PKA), protein kinase C and protein phosphatases, associating reversibly with the beta(2)-adrenergic receptor. PMID: 14657015
    34. AKAP12A may function as an important negative regulator of the survival pathway in human gastric cancer. PMID: 15258566
    35. AKAP12alpha belongs to a novel class of atypical SRF-dependent target genes PMID: 15590635
    36. AKAP12 localization is regulated by a hierarchy of targeting domains and the localization of AKAP12-assembled signaling complexes may be dynamically regulated PMID: 15923193
    37. Results suggest that gravin maintains a signaling complex that includes protein kinase A and phosphodiesterase 4D. PMID: 16642035
    38. Membrane binding of gravin, and thus function, can be reversed by calcium/calmodulin, which binds to the membrane and causes translocation of gravin from membrane to cytoplasm. PMID: 16762919
    39. We conclude that LPA-dependent increased level of cAMP in senescent human diploid fibroblasts is associated with increases in Gravin levels resulting in its increased binding with and activation of calcium-dependent PKC alpha/beta and AC4/6. PMID: 17081159
    40. The Src-binding peptide-(1-51) of gravin behaves as a dominant-negative for AKAP gravin regulation of beta2-adrenergic receptor resensitization/recycling PMID: 17200117
    41. Results suggest that AKAP12 may play an important role in tumor growth suppression by inducing apoptosis with the regulation of multiple molecules in the cell cycle progression. PMID: 17442483
    42. AKAP12 may induce BRB formation through antiangiogenesis and barriergenesis in developing human eye and defects in this mechanism can lead to loss of tight junction proteins and contribute to development of retinal pathologies such as retinoblastoma. PMID: 17442832
    43. The Gravin expression was found to be decreased in samples of acute leukaemia and was associated with an inferior overall survival. PMID: 17577780
    44. Hypermethylation of the AKAP12 promoter is associated esophageal carcinoma. PMID: 18199717
    45. AKAP12 in astrocytes induces barrier functions in human endothelial cells through protein kinase Czeta. PMID: 18397319
    46. These findings implicate AKAP12 in the regulation of cytokinesis progression, and suggest a novel role for AKAP12 tumor suppressor. PMID: 18554502
    47. Results suggest that AKAP12A may activate SREBP-2 by increasing cholesterol efflux, and is a novel regulator of cellular cholesterol metabolism. PMID: 18579430
    48. AKAP12 docks to the beta2-adrenergic receptor in response to agonist stimulation, later internalizes with agonist-induced receptors, and finally dissociates from the recycled, cell membrane-bound receptor. PMID: 18950703
    49. Data show that PKC activation resulted in redistribution of gravin and PKA constructs to the same subcellular site, and suggest that this response to PKC activity may mediate PKC dependent control of PKA activity. PMID: 19210988
    50. Data revealed that six polymorphisms of F10, PITRM1, PCSK2, JPH3, MYO7B, and AKAP12 were related (P<0.05) to the prevalence of chronic kidney disease. PMID: 19724895

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  • 亚细胞定位：
    Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Membrane; Lipid-anchor. Note=May be part of the cortical cytoskeleton.
  • 组织特异性：
    Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
  • 数据库链接：

    HGNC: 370

    OMIM: 604698

    KEGG: hsa:9590

    STRING: 9606.ENSP00000253332

    UniGene: Hs.371240