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AIFM2 Antibody

  • 货号：
    CSB-PA874794LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA874794LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Western Blot
      Positive WB detected in: A549 whole cell lysate
      All lanes: AIFM2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 41, 37 kDa
      Observed band size: 41 kDa
    • Immunofluorescence staining of HepG2 cells with CSB-PA874794LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) AIFM2 Polyclonal antibody
  • Uniprot No.：
    Q9BRQ8
  • 基因名：
    AIFM2
  • 别名：
    5430437E11Rik antibody; aifm2 antibody; AIFM2_HUMAN antibody; AMID antibody; Apoptosis inducing factor (AIF) homologous mitochondrion associated inducer of death antibody; Apoptosis inducing factor (AIF) like mitochondrion associated inducer of death antibody; Apoptosis inducing factor mitochondrion associated 2 antibody; Apoptosis-inducing factor 2 antibody; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death antibody; Apoptosis-inducing factor-like mitochondrion-associated inducer of death antibody; Cys51Stop antibody; HGNC11998 antibody; p53 responsive gene 3 antibody; p53 tumor suppressor antibody; p53-responsive gene 3 protein antibody; PRG3 antibody; TRP53 antibody; Tumor protein p53 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Apoptosis-inducing factor 2 protein (110-373AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，AIFM2 Antibody (CSB-PA874794LA01HU)，的标记方式是Non-conjugated。对于AIFM2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA874794LB01HU AIFM2 Antibody, HRP conjugated ELISA
    FITC CSB-PA874794LC01HU AIFM2 Antibody, FITC conjugated
    Biotin CSB-PA874794LD01HU AIFM2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    A NAD(P)H-dependent oxidoreductase involved in cellular oxidative stress response. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels. May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death. Capable of DNA binding in a non-sequence specific way.
  • 基因功能参考文献：
    1. Low AIFM2 expression is associated with T-cell lymphoblastic lymphoma. PMID: 28534937
    2. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type). PMID: 26063804
    3. HUHS1015 increased nuclear localization of apoptosis-inducing factor-homologous mitochondrion-associated inducer of death (AMID). PMID: 25244912
    4. induces caspase-independent apoptosis PMID: 11980907
    5. encodes a homologue of the apoptosis-inducing factor PMID: 12135761
    6. AMID is a p53-downstream gene involved in tumorigenesis. PMID: 15273740
    7. Reasults establish a link between coenzyme and DNA binding that likely impacts on the physiological role of AMID in cellular apoptosis. PMID: 15958387
    8. study of cellular localization of the endonuclease G, AIF & AMID during apoptosis using bioinformatics and image analysis PMID: 17347867
    9. AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro)viral) cytosolic DNA, thus contributing to the onset of apoptosis PMID: 17711848

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  • 亚细胞定位：
    Lipid droplet. Cell membrane; Lipid-anchor. Cytoplasm. Mitochondrion membrane. Nucleus.
  • 蛋白家族：
    FAD-dependent oxidoreductase family
  • 组织特异性：
    Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expres
  • 数据库链接：

    HGNC: 21411

    OMIM: 605159

    KEGG: hsa:84883

    STRING: 9606.ENSP00000312370

    UniGene: Hs.650680