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AGXT2 Antibody

  • 货号：
    CSB-PA866322LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: LO2 whole cell lysate
      All lanes: AGXT2 antibody at 4.9µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 58, 50 kDa
      Observed band size: 50 kDa
    • IHC image of CSB-PA866322LA01HU diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA866322LA01HU diluted at 1:700 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA866322LA01HU at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) AGXT2 Polyclonal antibody
  • Uniprot No.：
    Q9BYV1
  • 基因名：
    AGXT2
  • 别名：
    AGXT2 antibody; AGT2Alanine--glyoxylate aminotransferase 2 antibody; mitochondrial antibody; AGT 2 antibody; EC 2.6.1.44; antibody; R)-3-amino-2-methylpropionate--pyruvate transaminase antibody; EC 2.6.1.40 antibody; Beta-ALAAT II antibody; Beta-alanine-pyruvate aminotransferase antibody; D-AIBAT antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Alanine--glyoxylate aminotransferase 2, mitochondrial protein (196-328AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，AGXT2 Antibody (CSB-PA866322LA01HU)，的标记方式是Non-conjugated。对于AGXT2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA866322LB01HU AGXT2 Antibody, HRP conjugated ELISA
    FITC CSB-PA866322LC01HU AGXT2 Antibody, FITC conjugated
    Biotin CSB-PA866322LD01HU AGXT2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Can metabolize asymmetric dimethylarginine (ADMA) via transamination to alpha-keto-delta-(NN-dimethylguanidino) valeric acid (DMGV). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure.
  • 基因功能参考文献：
    1. We purified human AGXT2 from tissues of AGXT2 transgenic mice and demonstrated its ability to metabolize homoarginine to 6-guanidino-2-oxocaproic acid (GOCA). After incubation of HepG2 cells overexpressing AGXT2 with isotope-labeled homoarginine-d4 we were able to detect labeled GOCA in the medium PMID: 27752063
    2. study in a well-characterized rheumatoid arthritis population did not show an association between serum concentrations of dimethylarginines and genetic variants of the AGXT2 gene. PMID: 28357606
    3. AGXT2 rs37369 polymorphism is associated with increased risk for chronic heart failure, which may due to distinct disparities of alleles in asymmetric dimethylarginine degradation PMID: 27423328
    4. Single nucleotide polymorphism in AGXT2 gene is associated with renal dysfunction in patients with chronic heart failure. PMID: 28942034
    5. The Alanine-glyoxylate aminotransferase 2 p.V498L polymorphism is associated with both paroxysmal and chronic forms of atrial fibrillation in coronary angiographic patients without structural heart disease in ultrasound, and earlier age at onset of ischemic stroke in patients undergoing exercise stress testing. PMID: 26984639
    6. AGXT2 rs37369 polymorphism is associated with increased risk for CHD in smokers and in diabetes mellitus patients PMID: 24834905
    7. The AGXT2 genotype may be an important factor underlying atherosclerosis. PMID: 25620171
    8. The results of this study that the AGXT2 gene is not associated with schizophrenia in Japanese subjects. PMID: 24727203
    9. SNPs of AGXT2 affect plasma as well as urinary BAIB. PMID: 24586340
    10. AGXT2 has an important role in SDMA metabolism in humans and may additionally have an unanticipated role in the autonomic nervous system regulation of cardiac function. PMID: 24159190
    11. Alanine-glyoxylate aminotransferase-2 metabolizes endogenous methylarginines, regulates NO, and controls blood pressure. PMID: 23023372
    12. mitochondrially localized human AGXT2 is able to effectively metabolize ADMA in vivo resulting in decreased ADMA levels and improved endothelial NO production. PMID: 20018850
    13. Discusses the cloning of the rat gene. PMID: 7592550

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  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    Class-III pyridoxal-phosphate-dependent aminotransferase family
  • 数据库链接：

    HGNC: 14412

    OMIM: 210100

    KEGG: hsa:64902

    STRING: 9606.ENSP00000231420

    UniGene: Hs.34494