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ADRM1 Antibody

  • 货号：
    CSB-PA001396HA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse stomach tissue
      All lanes: ADRM1 antibody at 2.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 43 kDa
      Observed band size: 43 kDa
    • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA001396HA01HU at dilution of 1:100
    • Immunofluorescent analysis of MCF-7 cells using CSB-PA001396HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ADRM1 Polyclonal antibody
  • Uniprot No.：
    Q16186
  • 基因名：
    ADRM1
  • 别名：
    110 kDa cell membrane glycoprotein antibody; adhesion regulating molecule 1 antibody; Adhesion-regulating molecule 1 antibody; ADRM 1 antibody; Adrm1 antibody; ADRM1_HUMAN antibody; ARM 1 antibody; ARM-1 antibody; ARM1 antibody; Gp110 antibody; hRpn13 antibody; M(r) 110,000 surface antigen antibody; Proteasomal ubiquitin receptor ADRM1 antibody; proteasome regulatory particle non ATPase 13 antibody; Proteasome regulatory particle non-ATPase 13 antibody; Regulatory particle non ATPase 13 antibody; Rpn13 antibody; Rpn13 homolog antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Proteasomal ubiquitin receptor ADRM1 protein (2-407AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ADRM1 Antibody (CSB-PA001396HA01HU)，的标记方式是Non-conjugated。对于ADRM1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA001396HB01HU ADRM1 Antibody, HRP conjugated ELISA
    FITC CSB-PA001396HC01HU ADRM1 Antibody, FITC conjugated
    Biotin CSB-PA001396HD01HU ADRM1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. Within the complex, functions as a proteasomal ubiquitin receptor. Engages and activates 19S-associated deubiquitinases UCHL5 and PSMD14 during protein degradation. UCHL5 reversibly associate with the 19S regulatory particle whereas PSMD14 is an intrinsic subunit of the proteasome lid subcomplex.
  • 基因功能参考文献：
    1. Rpn13-Rpn2 complex structural analysis shows that RA190 targets hRpn13 and Uch37 through parallel mechanisms and at proteasomes, RA190-inactivated Uch37 cannot disassemble hRpn13-bound ubiquitin chains PMID: 28598414
    2. findings indicate that up-regulated ADRM1 was involved in intrahepatic cholangiocarcinoma (ICC) progression and suggest the potential clinical application of ADRM1 inhibitors (e.g., RA190 and KDT-11) for ICC treatment. PMID: 29913454
    3. We show that ADRM1 mRNA overexpression is an early event in high grade serous carcinoma of the ovary. This is associated with TP53 mutation and increased burden of misfolded proteins in carcinomas that likely renders the cancer cells particularly sensitive to RPN13 inhibitors. PMID: 28784174
    4. evidence that the interaction can mediate the association of Rpn13 and SGTA in a cellular context. PMID: 27827410
    5. The structures of proteasome substrate receptor complexes with the shuttle factors that deliver ubiquitinated proteins to proteasomes have been solved, namely human Rpn13 complexed with PLIC2 and Saccharomyces cerevisiae Rpn1 with Rad23. PMID: 27396824
    6. RPN13 binds ubiquitin with an affinity similar to that of other proteasome-associated ubiquitin receptors and that RPN2, ubiquitin, and the deubiquitylase UCH37 bind to RPN13 with independent energetics. PMID: 28442575
    7. regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. PMID: 26903513
    8. this work implicates hRpn13 and Uch37 in cell cycle progression, providing a rationale for their function in cellular proliferation and for the apoptotic effect of the hRpn13-targeting molecule RA190. PMID: 26907685
    9. the binding of SGTA to Rpn13 enables specific polypeptides to escape proteasomal degradation and/or selectively modulates substrate degradation. PMID: 26169395
    10. Data suggest that ADRM1 is involved in proliferation of acute leukemia cells; expression of ADRM1 is up-regulated in leukemia; knockdown of ADRM1 inhibits cell proliferation at G0/G1 phase of cell cycle but does not affect apoptosis/cell migration. PMID: 25896055
    11. findings implicate Rpn13 in linking parkin to the 26 S proteasome and regulating the clearance of mitochondrial proteins during mitophagy PMID: 25666615
    12. Data show that DEUBAD domain in RPN13 (ADRM1) activates ubiquitin thioesterase L5 (UCH-L5), and the related DEUBAD domain in INO80G (NFRKB) inhibits UCH-L5. PMID: 25702870
    13. Together, our findings suggest that the interaction of Psmd1 with Adrm1 is controlled by SUMOylation in a manner that may alter proteasome composition and function. PMID: 24910440
    14. ADRM1 is a candidate target gene in the chromosome 20q13.33 amplicon that may possibly be linked to development of gastric cancer PMID: 24968865
    15. Autoubiquitination of the 26S proteasome on Rpn13 regulates breakdown of ubiquitin conjugates. PMID: 24811749
    16. Inherent asymmetry in the 26S proteasome is defined by the ubiquitin receptor RPN13. PMID: 24429290
    17. mRNA and protein levels of ADRM1 were increased in hepatocellular carcinoma tissues and was parallel to the metastatic potential PMID: 22576803
    18. hRpn13 modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts. PMID: 22057889
    19. this study provides a possible mechanism of action in ovarian cancer for amplification and overexpression of ADRM1 PMID: 21432940
    20. In tumor cells non-phosphorylated DeltaNp63alpha failed to form protein complexes with Rpn13, allowing the latter to bind and target LKB1 into a proteasome-dependent degradation pathway, modulating cisplatin-induced autophagy. PMID: 21191146
    21. Phosphorylated TP63 induces transcription of RPN13, leading to NOS2 protein degradation PMID: 20959455
    22. Silencing of Adrm1 by RNA interference can significantly suppress proliferation of colorectal cancer cells through inducing apoptosis and arresting the cell cycle. PMID: 20137344
    23. show that Rpn13 is involved in inducible nitric oxide synthase degradation and is required for iNOS interaction with the deubiquitination protein UCH37. PMID: 20634424
    24. results suggest that there is different substrate specificity between S5a and hRpn13 at the level of delivery and S5a may be the major docking site for ERAD substrates. PMID: 20417181
    25. Rpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding. PMID: 20471946
    26. ARM-1 is a cytosolic protein associated with the plasma membrane. However, no cell surface expression of the protein was observed. These results suggest an indirect role of ARM-1 in adhesion rather than a direct role as an adhesion molecule itself.[ARM-1] PMID: 15819879
    27. Adrm1 has a specialised role in proteasome function. Identified Adrm1 as a novel component of the regulatory ATPase complex of the 26 S proteasome PMID: 16815440
    28. Neither Uch37 alone nor the Uch37-Adrm1 or Uch37-Adrm1-S1 complexes can hydrolyse di-ubiquitin efficiently; rather, incorporation into the 19S complex is required to enable processing of polyubiquitin chains. PMID: 16906146
    29. These results indicate that hRpn13 (Adrm1) is essential for the activity of UCH37. PMID: 16990800
    30. In human 26S proteasomes, hRpn13 appears to be important for the binding of UCH37 to the 19S complex and for efficient proteolysis. PMID: 17139257
    31. identification of a new ubiquitin receptor, Rpn13/ARM1, a known component of the proteasome PMID: 18497817
    32. ADRM1 overexpression was the most highly correlated with amplification and is associated with ovarian cancer. PMID: 18615678
    33. Adrm1 is potentially oncogenic and may play an important role in colon tumorigenesis PMID: 19148532
    34. these data suggest that Adrm1, a new Atp6v0d2-interacting protein, plays an important role in osteoclast differentiation, and in particular the fusion of preosteoclasts. PMID: 19818731

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    ADRM1 family
  • 数据库链接：

    HGNC: 15759

    OMIM: 610650

    KEGG: hsa:11047

    STRING: 9606.ENSP00000253003

    UniGene: Hs.90107