Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. ADH5 Antibody

ADH5 Antibody

  • 货号：
    CSB-PA001357LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Rat liver tissue
      All lanes: ADH5 antibody at 2μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 40 kDa
      Observed band size: 40 kDa
    • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA001357LA01HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001357LA01HU at dilution of 1:100
    • Immunofluorescent analysis of Hela cells using CSB-PA001357LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ADH5 Polyclonal antibody
  • Uniprot No.：
    P11766
  • 基因名：
    ADH5
  • 别名：
    ADH 3 antibody; ADH5 antibody; ADHX antibody; ADHX_HUMAN antibody; Alcohol dehydrogenase (class III) chi polypeptide antibody; alcohol dehydrogenase 5 (class III) chi polypeptide antibody; Alcohol dehydrogenase 5 antibody; Alcohol dehydrogenase class 3 antibody; Alcohol dehydrogenase class chi chain antibody; Alcohol dehydrogenase class III antibody; Alcohol dehydrogenase class-3 antibody; Alcohol dehydrogenase class-III antibody; class III alcohol dehydrogenase 5 chi subunit antibody; FALDH antibody; FDH antibody; formaldehyde dehydrogenase antibody; Glutathione dependent formaldehyde dehydrogenase antibody; Glutathione-dependent formaldehyde dehydrogenase antibody; GSH-FDH antibody; GSNOR antibody; hydroxymethyllutathione dehydrogenase antibody; S-(hydroxymethyl)glutathione dehydrogenase antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human Alcohol dehydrogenase class-3 protein (14-130AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ADH5 Antibody (CSB-PA001357LA01HU)，的标记方式是Non-conjugated。对于ADH5 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA001357LB01HU ADH5 Antibody, HRP conjugated ELISA
    FITC CSB-PA001357LC01HU ADH5 Antibody, FITC conjugated
    Biotin CSB-PA001357LD01HU ADH5 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol.
  • 基因功能参考文献：
    1. Data (including data from studies using knockout and transgenic mice) suggest that obesity and diabetes are accompanied by decreases in GSNOR activity in hepatocytes engendering nitrosative stress; obesity promotes S-nitrosylation of lysosomal proteins in liver, thereby impairing lysosomal enzyme activities and compromising autophagy. PMID: 29074597
    2. GSNOR represents the prototype enzyme to disclose how denitrosylation plays a crucial role in tuning NO-bioactivity and how much it deeply impacts on cell homeostasis and human pathophysiology. (Review) PMID: 28533171
    3. It was concluded that in HepG2 cells, ADH5 is a source of formate for de novo purine biosynthesis, especially during folate deficiency when folate-dependent formate production is limited. PMID: 28228507
    4. GSNOR expression has different effect on neuronal viability in dependence on the stimulus applied, and plays opposite roles in SH-SY5Y models of Parkinson's disease and amyotrophic lateral sclerosis PMID: 26491229
    5. ADH5 counteracts neuronal differentiation of neural stem cells and this effect can be reversed by pharmacological inhibition of ADH5. PMID: 24895131
    6. study compared individuals occupationally exposed to formaldehyde and controls to effects of XRCC3 Thr241Met, ADH5 Val309Ile and Asp353Glu polymorphisms; ADH5 polymorphisms did not show significant association with genotoxicity biomarkers PMID: 23355119
    7. N6022 binds in the GSNO binding pocket like a competitive inhibitor, although in kinetic assays it behaves with a mixed uncompetitive mode of inhibition (MOI) toward GSNO and a mixed competitive MOI toward formaldehyde adduct S-hydroxymethylglutathione. PMID: 22335564
    8. biological significance of SNPs rs11568816, rs17028487 and rs13832 PMID: 21920416
    9. Data suggest that GSNOR deficiency, through dysregulated S-nitrosylation, may promote hepatocellular carcinoma, possibly by inactivating a DNA repair system. PMID: 20371487
    10. Significant associations were found however, for reactions to alcohol with a SNP in ADH5 (rs6827292, p = .001) and a SNP just upstream of ADH5 (rs6819724, p = .0007) that is in strong LD with rs6827292. PMID: 20158305
    11. POZ domain of FBI1 represses transcription of ADH5. PMID: 12004059
    12. The structural determination of apo, binary alcohol, and inhibitory ternary FDH complexes provides new insight into the enzyme's metal-assisted catalysis and substrate-binding properties. PMID: 12196016
    13. Formation of a FDH-S-(hydroxymethyl)glutathione-NADH ternary complex causes movement of glutathione-dependent formaldehyde dehydrogenase catalytic domain toward the coenzyme-binding domain, and a change in active-site zinc coordination. PMID: 12484756
    14. No evidence that alcohol dehydrogenase 3genotype modifies risk related to alcohol and lung cancer. PMID: 14608084
    15. data suggest that genetic variation in S-nitrosoglutathione reductase (GSNOR) might play a role in asthma susceptibility PMID: 17543375
    16. A statistically significant increase of class III alcohol dehydrogenase isoenzymes was found in the sera of pancreatic cancer patients. PMID: 18095160
    17. GSNOR inhibitors to be novel tools for regulating nitric oxide bioactivity and assessing the role of SNOs in vivo. PMID: 19596685

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Zinc-containing alcohol dehydrogenase family, Class-III subfamily
  • 数据库链接：

    HGNC: 253

    OMIM: 103710

    KEGG: hsa:128

    STRING: 9606.ENSP00000296412

    UniGene: Hs.78989