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  4. lacZ Monoclonal Antibody

lacZ Monoclonal Antibody

  • 货号：
    CSB-MA009476A0m
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in Recombinant protein
      All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 117 kDa
      Observed band size: 130 kDa
    • Western Blot
      Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
      All lanes: lacZ antibody at 1:2000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 117 kDa
      Observed band size: 130 kDa
    • Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
    • Immunoprecipitating lacZ in 293T transfected whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate
      Lane 2: CSB-MA009476A0m (2µl) + 293T transfected whole cell lysate (500µg)
      Lane 3: 293T transfected whole cell lysate (20µg)
      For western blotting, the blot was detected with CSB-MA000021M0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
    • Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.
  • 其他：

产品详情

  • 产品描述：

    The production process of this lacZ monoclonal antibody involves immunizing mice with the recombinant Escherichia coli Beta-galactosidase protein (2-1024aa), followed by isolation splenocytes from immunized mice and fusion of splenocytes with myeloma cells to form hybridomas. The selected lacZ antibody-secreting hybridomas were cultured to harvest large amounts of lacZ monoclonal antibodies. This lacZ monoclonal antibody was purified with protein G with a purity of above 95%. It can be used for the detection of Escherichia coli lacZ protein in ELISA, WB, IF, IP, and FC applications.

    lacZ protein is a β-galactosidase enzyme that hydrolyzes the glycosidic bond between a galactose and a glucose or galactose. Its main function is to break down lactose into glucose and galactose, which can then be utilized by the cell for energy production. Additionally, the lacZ protein is often used as a reporter gene in molecular biology experiments to detect the expression of genes of interest.

  • 产品名称：
    Mouse anti-Escherichia coli lacZ Monoclonal antibody
  • Uniprot No.：
    P00722
  • 基因名：
    lacZ
  • 别名：
    lacZ antibody; b0344 antibody; JW0335Beta-galactosidase antibody; Beta-gal antibody; EC 3.2.1.23 antibody; Lactase antibody
  • 宿主：
    Mouse
  • 反应种属：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli Beta-galactosidase protein (2-1024AA)
  • 免疫原种属：
    Escherichia coli
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    3B7D5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:64000
    IF 1:50-1:200
    IP 1ul-4ul
    FC 1:100-1:300
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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