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USP6 Monoclonal Antibody

  • 货号：
    CSB-MA025747A0m
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: USP6 antibody at 1:1000
      Lane 1: MCF-7 whole cell lysate
      Lane 2: THP-1 whole cell lysate
      Lane 3: SH-SY5Y whole cell lysate
      Lane 4: Jurkat whole cell lysate
      Lane 5: HEK293 whole cell lysate
      Lane 6: Hela whole cell lysate
      Secondary
      Goat polyclonal to Mouse IgG at 1/20000 dilution
      Predicted band size: 89, 122, 159 KDa
      Observed band size: 89, 122, 159 KDa
      Exposure time: 1min
    • Immunofluorescence staining of HeLa cells with CSB-MA025747A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    After immunizing mice with recombinant human USP6 protein (348-535aa), B cells were isolated from the mouse spleen and then fused with myeloma cells to form hybridomas. USP6 antibody-producing hybridomas were selected from these hybridomas and cultured to obtain monoclonal antibodies against USP6. This USP6 monoclonal antibody is highly specific to human species and can be used in ELISA, WB, and IF applications. The antibody is purified using protein G affinity chromatography, with a purity of over 95%.

    USP6 is a deubiquitinating enzyme implicated in various cellular functions, including protein degradation, DNA repair, and signal transduction. USP6 is well-known for the involvement in bone remodeling. It has been found to be overexpressed in certain types of bone tumors, such as aneurysmal bone cysts, and has been implicated in the regulation of osteoblast differentiation and bone growth. Additionally, USP6 has also been linked to the regulation of cell proliferation, migration, and invasion, and is involved in the maintenance of genomic stability.

  • 产品名称：
    Human anti-Homo sapiens (Human) USP6 Monoclonal antibody
  • Uniprot No.：
    P35125
  • 基因名：
    USP6
  • 别名：
    Deubiquitinating enzyme 6 antibody; HRP1 antibody; Proto-oncogene TRE-2 antibody; TRE17 antibody; TRE2 antibody; Ubiquitin carboxyl-terminal hydrolase 6 antibody; Ubiquitin specific protease 6 antibody; Ubiquitin thiolesterase 6 antibody; Ubiquitin-specific-processing protease 6 antibody; UBP6_HUMAN antibody; USP6 antibody
  • 宿主：
    Human
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Ubiquitin carboxyl-terminal hydrolase 6 protein (348-535AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    3C3G2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
  • 基因功能参考文献：
    1. None of the genitourinary pseudosarcomatous myofibroblastic proliferations was found to harbour USP6 (0/12), ROS1 (0/8) or ETV6 (0/7) rearrangements PMID: 29617048
    2. we identified seven novel fusion partners for USP6 in nodular fasciitis, highlighting the importance of USP6 expression and promoter-swapping fusions in the etiology of this neoplasm PMID: 28752842
    3. Report the presence of USP6 rearrangements in a subset of cellular fibroma of tendon sheath. PMID: 27125357
    4. our studies highlight Jak1 as the first identified substrate for USP6, and they offer a mechanistic rationale for the clinical investigation of Jak and STAT3 inhibitors as therapeutics for the treatment of bone and soft tissue tumors along with other neoplasms driven by USP6 overexpression PMID: 27440725
    5. Molecular analyses revealed the presence and amplification of the novel PPPR6-USP6 gene fusion, which resulted in USP6 mRNA transcriptional upregulation. These findings further support the oncogenic role of the USP6 protease in mesenchymal neoplasia and expand the biologic potential of Nodular fasciitis PMID: 27113271
    6. It was shown that TRE17 activates the classical NF-kappa B pathway through an atypical mechanism that does not involve IkappaB degradation. Optimal activation of NF-kappa B by TRE17 required both catalytic subunits of IkappaB kinase. PMID: 22081069
    7. USP6 fluorescence in-situ hybridization is a useful ancillary test in cases where nodular fasciitis is a potential diagnostic consideration. PMID: 27271298
    8. the deubiquitylase ubiquitin-specific protease 6 (USP6) as a potent activator of Wnt signaling. USP6 enhances Wnt signaling by deubiquitylating Fzds, thereby increasing their cell-surface abundance. PMID: 27162353
    9. TRE17/USP6 regulates ubiquitylation and trafficking of cargo proteins that enter cells by clathrin-independent endocytosis PMID: 25179595
    10. 8 of the 9 giant cell reparative granulomas from hands and feet showed rearrangements of the USP6 gene compared with none of 8 gnathic lesions PMID: 24742829
    11. we discuss the clinicopathologic features, molecular pathology, and pathogenesis of ABC and nodular fasciitis in relation to USP6 PMID: 23769422
    12. identification of a USP6 gene rearrangement is helpful in making a diagnosis of nodular fasciitis. PMID: 23748914
    13. manipulating USP6 expression levels alters the ability of cells to migrate and to divide. Cell proliferation and progression through cytokinesis depend on USP6 expression PMID: 22188517
    14. TRE17 is sufficient to initiate tumorigenesis, identify MMPs as novel TRE17 effectors that likely contribute to aneurysmal bone cyst pathogenesis. PMID: 20418905
    15. TRE17 coprecipitated specifically with the active forms of Cdc42 and Rac1 in vivo. TRE17 is part of a novel effector complex for Cdc42 and Rac1, potentially contributing to their effects on actin remodeling. PMID: 12612085
    16. Complementation tests in yeasts indicate that Tre2 codes for a nonfunctional RabGAP. PMID: 14521938
    17. Deregulated USP6 transcription is associated with aneurysmal bone cyst PMID: 15026324
    18. primary aneurysmal bone cysts are mesenchymal neoplasms exhibiting USP6 and/or CDH11 oncogenic rearrangements PMID: 15509545
    19. TRE17 associates directly with Arf6 in its GDP- but not GTP-bound state. PMID: 15509780
    20. Tre2 oncogene seems to encode a nonfunctional Rab GAP. As regions flanking the TBC domain may be crucial for catalytic activity PMID: 16099424
    21. Ca2+/CaM has a role in regulating ubiquitination through direct interaction with TRE17 PMID: 16127172
    22. The lack of secondary structure of the region flanking the TBC domain in TRE2 may explain why this region plays a role in the lack of GAP activity, even when a potentially functional TBC domain is present. PMID: 17701273
    23. No USP6 rearrangements were found in cherubism or brown tumors. USP6 rearrangements were identified in 2 patients with myositis ossificans. PMID: 18265974

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  • 相关疾病：
    A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation (PubMed:15026324).
  • 亚细胞定位：
    Cell membrane. Cytoplasm. Endosome. Note=Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
  • 蛋白家族：
    Peptidase C19 family
  • 组织特异性：
    Testis specific. Expressed in various cancer cell lines.
  • 数据库链接：

    HGNC: 12629

    OMIM: 604334

    KEGG: hsa:9098

    STRING: 9606.ENSP00000250066

    UniGene: Hs.448851