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SPP1 Monoclonal Antibody

  • 货号：
    CSB-MA069021A0m
  • 规格：
    ￥1320
  • 图片：
    • Immunohistochemical of paraffin-embedded human colon cancer using CSB-MA069021A0m at dilution of 1:200
    • Immunohistochemical of paraffin-embedded human Kidney tissue using CSB-MA069021A0m at dilution of 1:200
    • All Lanes Mouse anti human OPN monoclonal antibody at at 2μg/ml
      Lane 1:mouse kidney tissue lysate
      Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution
      Predicted band size : 33,36,34kDa
      Observed band size: 50kDa
  • 其他：

产品详情

  • 产品描述：

    The SPP1 monoclonal antibody is produced through a process of immunizing mice with recombinant human osteopontin, isolating B cells from the spleen of the immunized mice, and then fusing these B cells with myeloma cells. The resulting hybridomas are screened for those that produce antibodies specific to SPP1. The selected hybridomas are then cultured in the mouse abdominal cavity, and the SPP1 monoclonal antibodies are harvested and purified using a protein G affinity chromatography from the mouse ascites. The final purified antibody has a high level of purity, over 95%. It can be used for the detection of human osteopontin in ELISA, WB, and IHC applications.

    SPP1, also known as osteopontin, mainly regulates cellular activities such as adhesion, migration, and signaling. It plays a critical role in many physiological and pathological processes, including bone metabolism, immune response, inflammation, tissue repair, and tumor progression.

  • 别名：
    Bone sialoprotein 1,Nephropontin,Secreted phosphoprotein 1,SPP-1,Urinary stone protein,Uropontin, BNSP, OPN, PSEC0156
  • 宿主：
    mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Osteopontin
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%,Protein G purified
  • 克隆号：
    1B2C2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    liquid
  • 应用范围：
    ELISA,WB,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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