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SLN Monoclonal Antibody

  • 货号：
    CSB-MA021780A0m
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-MA021780A0m diluted at 1:60 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Overlay Peak curve showing U87 cells stained with CSB-MA021780A0m (red line) at 1:60. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    Using recombinant human SLN protein (1-31aa) as the immunogen, the mouse is immunized to isolate the B cells. Fusing mouse B cells with myeloma cells to get the hybridomas, screening for the desired hybridoma cell line that produces the SLN antibody, and then culturing the selected hybridomas in the mouse abdominal cavity. The SLN monoclonal antibody is purified from the mouse ascites using protein G affinity chromatography, resulting in a purity of over 95%. This unconjugated IgG2b antibody is suitable for detecting the human SLN protein in ELISA, WB, IF, and FC applications.

    Sarcolipin (SLN) is a small peptide that regulates the activity of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump in skeletal muscle cells. Specifically, sarcolipin inhibits the activity of SERCA, which leads to reduced uptake of calcium ions into the sarcoplasmic reticulum and slower muscle relaxation. It plays an important role in regulating the contractility of skeletal muscle cells and thus overall muscle function. Dysfunction or deficiency of sarcolipin has been associated with muscle disorders such as skeletal muscle weakness and heart failure.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) SLN Monoclonal antibody
  • Uniprot No.：
    O00631
  • 基因名：
    SLN
  • 别名：
    SLN antibody; Sarcolipin antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human SLN protein (1-31AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    14G1A12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB WB：1:1000-1:5000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Reversibly inhibits the activity of ATP2A1 and ATP2A2 in sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in muscle. Required for muscle-based, non-shivering thermogenesis.
  • 基因功能参考文献：
    1. Structure-Function Relationship of the SERCA Pump and Its Regulation by Phospholamban and Sarcolipin. PMID: 29594859
    2. Self-assembling study of sarcolipin and its mutants in multiple molecular dynamic simulations has been reported. PMID: 28241400
    3. These results suggest that sAnk1 interacts with SLN both directly and in complex with SERCA1 and reduces SLN's inhibitory effect on SERCA1 activity. PMID: 28487373
    4. analysis of transmembrane dynamics of the Thr-5 phosphorylated sarcolipin pentameric channel PMID: 27378083
    5. Phospholamban and sarcolipin are membrane proteins that differentially regulate SERCA function. (Review) PMID: 26743715
    6. Molecular dynamics simulations help to clarify and to understand better the SLN pentamer channel that had a hydrophobic gate and could switch Na(+) and Cl(-) ion permeability by hydrated and vacuum states. PMID: 26522287
    7. Although SLN and PLB binding to SERCA have different functional outcomes on the coupling efficiency of SERCA, both proteins decrease the apparent Ca(2+) affinity of the pump, suggesting that SLN and PLB inhibit SERCA by using a similar mechanism. PMID: 25983321
    8. SLN and PLN are co-expressed in most fibers, which suggests that super-inhibition of SERCAs may be physiologically important in the regulation of intracellular Ca2+ in human skeletal muscle. PMID: 24358354
    9. The C-terminal tail of SLN is a distinct, essential domain in the regulation of SERCA. PMID: 23362265
    10. The selective downregulation of SLN and enhanced sarcoplasmic reticulum Ca(2+) uptake in human AF suggest that SLN downregulation could play an important role in abnormal intracellular Ca(2+) cycling in atrial pathology. PMID: 21640081
    11. sarcolipin binds to phospholamban and inhibits polymerization PMID: 12032137
    12. sarcolipin is likely to be an atrial chamber-specific regulator of Ca2+ cycling in heart PMID: 12645548
    13. role in regulating sarco(endo)plasmic reticulum Ca2+-ATPase by binding to transmembrane helices alone or in association with phospholamban PMID: 12692302
    14. Gene expression of SLN was studied in children with congenital heart defects. PMID: 17515962

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  • 亚细胞定位：
    Sarcoplasmic reticulum membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Sarcolipin family
  • 数据库链接：

    HGNC: 11089

    OMIM: 602203

    KEGG: hsa:6588

    STRING: 9606.ENSP00000304707

    UniGene: Hs.334629