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POU2AF1 Monoclonal Antibody

  • 货号：
    CSB-MA181399
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-MA181399 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-MA181399 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品名称：
    Mouse anti-Homo sapiens (Human) POU2AF1 Monoclonal antibody
  • Uniprot No.：
    Q16633
  • 基因名：
    POU2AF1
  • 别名：
    B cell Oct binding protein 1 antibody; B cell specific coactivator OBF 1 antibody; B cell specific coactivator OBF1 antibody; B-cell-specific coactivator OBF-1 antibody; BOB 1 antibody; BOB-1 antibody; OBF 1 antibody; OBF1 antibody; OBF1_HUMAN antibody; OCA B antibody; OCA-B antibody; OCAB antibody; OCT binding factor 1 antibody; OCT-binding factor 1 antibody; POU class 2 associating factor 1 antibody; POU domain class 2 associating factor 1 antibody; POU domain class 2-associating factor 1 antibody; Pou2af1 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from human BOB.1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2a, Kappa
  • 纯化方式：
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator.
  • 基因功能参考文献：
    1. The number of follicular B2 lymphocytes and expression of the B-cell-specific transcriptional coactivator OcaB increase with age in spleen and in intra-abdominal epididymal white adipose tissue (eWAT), concomitantly with higher circulating levels of IgG and impaired glucose homeostasis. PMID: 29496744
    2. These findings demonstrate for the first time that functional polymorphism in the 3'-UTR of POU2AF1 is associated with susceptibility, and that single-nucleotide polymorphisms interaction with hsa-miR-633 affects gene expression and increases the risk of lymphoma. PMID: 28345816
    3. Oct2 and Bob1 are very reliable in determining B cell lineage in the absence of expression of other pan-B cell markers PMID: 27319306
    4. these findings suggest a novel function of POU2AF1 as a potential regulator of host defense genes in the human airway epithelium. PMID: 26927796
    5. It has been identified as a new disease susceptibility gene among Japanese. Though different from Europeans, it is indicated that a B lymphocyte differentiation route shares a common disease developing process. [Review] PMID: 24005100
    6. Two significant susceptibility loci, TNFSF15 (rs4979462) and POU2AF1 (rs4938534) (combined odds ratio [OR] = 1.56, p = 2.84 x 10(-14) for rs4979462. PMID: 23000144
    7. genetic polymorphism is associated with common variable immunodeficiency PMID: 21905497
    8. Data show that Igh 3' enhancer-bound OCA-B and promoter-bound TFII-I mediate promoter-enhancer interactions, in both cis and trans, that are important for Igh transcription. PMID: 21549311
    9. On multivariate analysis, co-expression of OCT-2/BOB.1 remained predictive for achievement of complete remission and increased risk of relapse. PMID: 20141429
    10. BOB.1 may be helpful marker in the differential diagnosis of classical Hodgkin's lymphoma and primary mediastinal B-cell lymphoma PMID: 20102401
    11. The expression of the octamer cofactor gene OBF-1 (Bob1/OCA-B) is sufficient to override the silencing effects of the B29 silencer, indicating that OBF-1 plays a critical role in B cell-specific B29 promoter expression. PMID: 11907094
    12. enhances transcriptional potential of Oct1 PMID: 12727885
    13. OCA-B sustains expression of the immunoglobulin-secreting program when T lymphoma and plasmacytoma lines are fused, requiring Oct-2 coregulator for its function. PMID: 14662861
    14. POU2AF1 was observed to be differentially expressed in the cells of patients with chronic lymphocytic leukemia. PMID: 15672409
    15. Alteration of the BOB1 locus does not correlate with its suppressed expression in Hodgkin lymphoma. PMID: 15796964
    16. Novel germ cell markers BOB1 were significantly upregulated in seminoma specimens, compared to normal testes. PMID: 17785371
    17. Oct-2 and its cofactor Bob-1 have an important function in mediating the IgH enhancer-bcl-2 promoter region interactions PMID: 18695675

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  • 相关疾病：
    A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    POU2AF1 family
  • 组织特异性：
    B-cell specific. Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine.
  • 数据库链接：

    HGNC: 9211

    OMIM: 601206

    KEGG: hsa:5450

    STRING: 9606.ENSP00000376786

    UniGene: Hs.654525