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  4. KRT14 Monoclonal Antibody

KRT14 Monoclonal Antibody

  • 货号：
    CSB-MA589883
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-MA589883 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-MA589883 diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    The production of the KRT14 monoclonal antibody starts with the immunization of mice using a synthesized peptide derived from human KRT14. After that, B cells are isolated from the spleen of the mice and fused with myeloma cells to generate hybridomas. The resulting hybridomas are screened to select out those that only produce antibodies that are specific to KRT14. Once specific hybridomas are identified, they are cultured in the mouse's abdominal cavity. The KRT14 monoclonal antibodies are purified from mouse ascites through affinity chromatography using a specific immunogen. The purified KRT13 monoclonal antibody is a mouse monoclonal antibody that specifically reacts with human KRT14 protein and can be utilized for ELISA and IHC applications. KRT14 is specifically expressed in the basal cells of stratified epithelia, such as the epidermis of the skin.

    The main function of KRT14 is to provide mechanical strength to the epithelial cells and protect them from mechanical stress, while also playing a role in cell signaling, adhesion, and migration. Mutations in the KRT14 gene have been associated with several skin disorders, including epidermolysis bullosa simplex, a condition characterized by skin blistering and erosion.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) KRT14 Monoclonal antibody
  • Uniprot No.：
    P02533
  • 基因名：
  • 别名：
    CK 14 antibody; CK-14 antibody; ck14 antibody; Cytokeratin 14 antibody; Cytokeratin-14 antibody; Cytokeratin14 antibody; Dowling Meara antibody; EBS3 antibody; EBS4 antibody; Epidermolysis bullosa simplex antibody; K14 antibody; K1C14_HUMAN antibody; Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody; Keratin 14 antibody; Keratin antibody; Keratin type I cytoskeletal 14 antibody; Keratin, type I cytoskeletal 14 antibody; Keratin-14 antibody; Keratin14 antibody; Koebner antibody; Krt 14 antibody; Krt14 antibody; NFJ antibody; OTTHUMP00000164624 antibody; type I cytoskeletal 14 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from human Cytokeratin 14
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1, Kappa
  • 纯化方式：
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
  • 基因功能参考文献：
    1. High CK14 expression is associated with lymph node metastasis in oral squamous cell carcinoma. PMID: 30066921
    2. Efficient homology-directed repair of a dominant negative KRT14 mutation via CRISPR/Cas9 nickases in epidermolysis bullosa simplex patients' keratinocytes has been reported. PMID: 28888469
    3. Immunocytochemical staining using cocktail antibody targeting p63/CK14 was useful for the differential diagnosis of FA and DCIS in FNAC of the breast. PMID: 28685877
    4. the findings show that squamous and micropapillary bladder cancers have different expression patterns of CK14 and FOXA1 and suggest that they arise from distinct precursors. PMID: 28721490
    5. PADI4 contributes to gastric tumorigenesis by upregulating CXCR2, KRT14 and TNF-alpha expression. PMID: 27556695
    6. The novel c.1234A>G(p.Ile412Val) mutation of the KRT14 gene is probably responsible for the disease. PMID: 28777847
    7. Keratin14/p63-positive epithelial proliferations suggest benign breast disease. PMID: 28630050
    8. K14 was coexpressed with alphav-integrin in fetal and adult corneas and cultured corneolimbal epithelium, and colony-forming efficiency (an indicator of stem cell activity) was similar in cells from both sources. PMID: 26956898
    9. Loss of keratin 14 is associated with epidermolysis bullosa. PMID: 27798626
    10. contributes to collective invasion of salivary adenoid cystic carcinoma and may be a biomarker of worse prognosis PMID: 28152077
    11. vimentin regulates the differentiation switch via modulation of K5/K14 expression. Moreover, because there was a significant correlation between high vimentin-K14 expression and recurrence/poor survival in oral cancer patients, vimentin-K14 together may prove to be the novel markers for the prognostication of human oral cancer. PMID: 28225793
    12. We concluded that smoking habits were capable of inducing changes in global DNA methylation, miR-9-3 methylation status and K19 expression. PMID: 27543926
    13. data demonstrates that keratinocyte migration requires the interaction between vimentin and keratins at the -YRKLLEGEE- sequence at the helical 2B domain of viment PMID: 27072292
    14. findings reveal K14 as a key regulator of metastasis and establish the concept that K14(+) epithelial tumor cell clusters disseminate collectively to colonize distant organs PMID: 26831077
    15. all keratins tested, except for keratin 14, were evenly expressed in all trophoblast cells. Keratin 14 was expressed in a subset of CK7 positive cells PMID: 26430881
    16. report a family with a novel heterozygous missense mutation p.Leu418Gln in the KRT14 gene causing epidermolysis bullosa simplex with variable phenotype PMID: 24981776
    17. investigated a family in which 1 of 3 children was diagnosed with a localized form of epidermolysis bullosa simplex and there was no family history of blistering; results argue against parental somatic and germline mosaicism in the family and suggest the novel p.Val270Ala mutation in KRT14 also represents a de novo event which occurred in the proband PMID: 23774754
    18. KRT14 protein genetic mutation is a good indicator of disease progression in patient diagnosed with epidermolysis bullosa simplex. PMID: 25961909
    19. Analysis of K14 variants with single or multiple substitutions of cysteine residues points to a spatial and temporal hierarchy in how Cys-4/Cys-40 and Cys-367 regulate keratin assembly in vitro and filament dynamics in live keratinocytes PMID: 26216883
    20. BerEp4 alone is unreliable for differentiation between BCCm (basal cell carcinoma with squamous metaplasia) and bSCC (basaloid squamous cell carcinoma). The addition of either CK14 or CK17 will augment BCCm versus bSCC differential diagnosis. PMID: 24168496
    21. hypothesize a positive feedback model in which mutant (R125P) K14 triggers JNK signalling, leading to increased AP1-dependent expression of K14, which in turn amplifies JNK signalling further PMID: 23528216
    22. p53 acts as a co-repressor to down-regulate K14 expression by binding to SP1. PMID: 22911849
    23. p53 acts as a co-repressor to down-regulate K14 expression by binding to SP1 during epidermal cell differentiation. PMID: 22911849
    24. One isoform of p63, TAp63alpha, can activate an epidermal basal cell marker, keratin 14. PMID: 22577164
    25. Mutant K14-R125P filaments and/or networks in human keratinocytes are mechanically defective in their response to large-scale deformations. PMID: 22363617
    26. This study adds two more novel recessive mutations in this gene associated with epidermolysis bullosa simplex. This is the first occurence in a Mediterranean population. PMID: 21623745
    27. Fascin and CK14 are highly expressed in squamous cell carcinoma, compared with other histological types of carcinoma. PMID: 21223690
    28. keratin 14 functional knockout causes severe recessive epidermolysis bullosa simplex, and questions the haploinsufficiency model of Naegeli-Franceschetti-Jadassohn syndrome PMID: 21734713
    29. Mutation analysis of an epidermolysis bullosa simplex family revealed that affected individuals were heterozygous for a, to our knowledge, previously unreported mutation of c.1237G>C (p.Ala413Pro) in KRT14. PMID: 21593775
    30. heterozygous G to A transition was found at nucleotide postion 1231 in exon 6 of KRT14 in family with epidermolysis bullosa simplex, generalized PMID: 21413954
    31. autoantibodies in Scurfy mice and patients with IPEX target keratin 14 PMID: 20147963
    32. analysis of a keratin 14 hotspot mutation in the Dowling-Meara type of epidermolysis bullosa simplex PMID: 19854623
    33. new heterozygous amino acid substitution polymorphism in the variable keratin 14 N-terminal head domain (KRT14:c.88C>T, p.Arg30Cys) PMID: 19797037
    34. A spontaneous CD8 T cell-dependent autoimmune disease to an antigen expressed under the human keratin 14 promoter. PMID: 12165543
    35. Three novel KRT14 mutations identified in 9 Epidermolysis bullosa simplex patients. PMID: 12655565
    36. investigated novel KRT14 missense mutations in epidermolysis bullosa simplex in a cellular expression system in order to analyse their effects on the keratin cytoskeleton PMID: 12930305
    37. Keratin 14 has a role in binding to TNFalpha receptor-associated death domain (TRADD), and in susceptibility of keratinocytes to caspase-8-mediated apoptosis PMID: 14660619
    38. novel recessive missense mutation in epidermolysis bullosa simplex PMID: 15654986
    39. Novel mutations within KRT14 are associated with epidermolysis bullosa simplex. PMID: 16786515
    40. Heterozygous nonsense or frameshift mutations in KRT14 were found to segregate with Naegeli-Franceschetti-Jadassohn syndrome or dermatopathia pigmentosa reticularis trait in five families. PMID: 16960809
    41. These studies provide a potential mechanism by which deltaNp63 directly governs the expression of K14 in a keratinocyte-specific manner. PMID: 17159913
    42. study presents a missense mutation in exon 1 of K14, R125C, identified in the affected individuals of a Chinese family with epidermolysis bullosa simplex-Dowling-Meara (EBS-DM) PMID: 17659012
    43. Better basal gene expression was observed by co-cultured respiratory epithelial cells compared to dispase dissociated cells. PMID: 17891046
    44. K14 and K16 were detected in the tumour cells, suggesting differentiation towards the outer root sheath beneath the orifice of the sebaceous duct. PMID: 18005116
    45. Naegeli-Franceschetti-Jadassohn syndrome results from haploinsufficiency for K14 and increased susceptibility of keratinocytes to pro-apoptotic signals may be involved in the pathogenesis of this ectodermal dysplasia syndrome PMID: 18049449
    46. Transgenic mice were generated using the keratin-14 promoter/enhancer to direct expression of wild-type human CXCR2 (K14hCXCR2 WT) or mutant CXCR2. PMID: 18505935
    47. expression of human K14 initiates squamous differentiation program in the mouse lung, but fails to promote squamous maturation PMID: 18701433
    48. Including the present case, 8 of the 13 families have the R125C or R125H mutation; eight have mutations in KRT14, and five have mutations in KRT5 PMID: 18717745
    49. Cataracts in transgenic mice caused by a human papillomavirus type 18 E7 oncogene driven by KRT1-14 are reported. PMID: 18723014
    50. Infection by HPV may alter the differentiation status of the epidermis, leading to a major expression of cytokeratin 14 PMID: 19515043

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  • 相关疾病：
    Epidermolysis bullosa simplex, Dowling-Meara type (DM-EBS); Epidermolysis bullosa simplex, Weber-Cockayne type (WC-EBS); Epidermolysis bullosa simplex, Koebner type (K-EBS); Epidermolysis bullosa simplex, autosomal recessive 1 (EBSB1); Naegeli-Franceschetti-Jadassohn syndrome (NFJS); Dermatopathia pigmentosa reticularis (DPR)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in the corneal epithelium (at protein level). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the
  • 数据库链接：

    HGNC: 6416

    OMIM: 125595

    KEGG: hsa:3861

    STRING: 9606.ENSP00000167586

    UniGene: Hs.654380