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  4. IFNLR1 Monoclonal Antibody

IFNLR1 Monoclonal Antibody

  • 货号：
    CSB-MA816871A0m
  • 规格：
    ￥1320
  • 图片：
    • Immunofluorescence staining of Hela cells with（CSB-MA816871A0m）at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Immunofluorescence staining of U251 cells with（CSB-MA816871A0m）at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    After immunizing mice with the recombinant IFNLR1 protein (21-228aa), B cells were isolated from the mouse spleen and fused with myeloma cells to create hybridomas. The hybridomas that produced the IFNLR1 monoclonal antibody were selected and then cultured in the mouse abdominal cavity. The IFNLR1 monoclonal antibody was purified from the collected mouse ascites using protein A affinity chromatography. It has a purity of over 95%. The resulting antibody is highly specific to human IFNLR1 protein and is suitable for ELISA and IF applications.

    IFNLR1 mainly mediates the antiviral response to viral infections. Upon activation by IFN-lambda, IFNLR1 forms a heterodimer with the IL10RB protein, leading to the activation of the JAK-STAT signaling pathway. This results in the transcriptional upregulation of interferon-stimulated genes (ISGs) that are involved in innate immunity and antiviral defense. Additionally, IFNLR1 signaling has also been implicated in various inflammatory diseases, such as psoriasis and inflammatory bowel disease.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) IFNLR1 Monoclonal antibody
  • Uniprot No.：
    Q8IU57
  • 基因名：
  • 别名：
    IFNLR1 antibody; IL28RA antibody; LICR2 antibody; Interferon lambda receptor 1 antibody; IFN-lambda receptor 1 antibody; IFN-lambda-R1 antibody; Cytokine receptor class-II member 12 antibody; Cytokine receptor family 2 member 12 antibody; CRF2-12 antibody; Interleukin-28 receptor subunit alpha antibody; IL-28 receptor subunit alpha antibody; IL-28R-alpha antibody; IL-28RA antibody; Likely interleukin or cytokine receptor 2 antibody; LICR2 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant IFNLR1 protein (21-228AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    5G12F3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state. Determines the cell type specificity of the lambda interferon action. Shows a more restricted pattern of expression in the epithelial tissues thereby limiting responses to lambda interferons primarily to epithelial cells of the respiratory, gastrointestinal, and reproductive tracts. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium.
  • 基因功能参考文献：
    1. IFN-lambda4 suppressed HIV infection of macrophages. This IFN-lambda4-mediated HIV inhibition was compromised by the antibodies against IFN-lambda receptor complex, IFN-lambdaR1/IL-10R2. PMID: 30247785
    2. Significant associations were observed for 4 variants in IFNAR2, IFNLR1 with hepatitis B virus infection, and IFNLR1-rs4649203 was associated with hepatitis B recovery. Moreover, the s demonstrated the clear relevance of 5 polymorphisms in IFNA1, IFNA2, IFNL4 with hepatocellular carcinoma. PMID: 29080269
    3. In this structure, the receptor peptide forms an 85-A-long extended chain, in which both the previously identified box1 and box2 regions bind simultaneously to the FERM and SH2-like domains of JAK1. PMID: 27725180
    4. Results show that IFNLR1 box1 and box2 motifs are required for stable complex formation with JAK1 and the crystal structure of JAK1 reveals IFNLR1 box1 binding site in the FERM domain. Also, structure-based mutagenesis reveals IFNLR1 PxxLxF motif that is essential for binding. Structure of JAK1 with IL10RA box1 indicates conservation among class II receptors. PMID: 27133025
    5. Selective expression of IFNlambdaR1 was considered to be a plus point towards the targeted anti-viral activity of IFNlambdas but artificial control on its expression will surely make IFNlambdas a better drug with enhanced activity. PMID: 27166550
    6. expression of IL-28RA mRNA may be correlated with the pathogenesis of systemic lupus erythematosus PMID: 25911176
    7. Polymorphism within the IL-28R1, but not in IL-28B, is associated with benign prostatic hyperplasia and lower urinary tract symptoms in Chinese. PMID: 26400298
    8. Results show that the distribution of IL10RB and IL28RA genotypes among the Hepatitis C virus-infected and control groups did not differ significantly. PMID: 24144988
    9. This study is the first to show the activation of a type III interferon response in low-risk human papillomavirus positive cervical cells and suggests that the lack of this response may be related to lesion progression. PMID: 24510368
    10. IL28RA polymorphism (rs10903035) seems to be implicated in the glucose homeostasis because AA genotype increases the likelihood of IR, but this association was different depending on hepatic fibrosis and HCV genotype. PMID: 24438680
    11. Epigenetic reprogramming of IFNLR1-mediated response potentiates antiviral activity and suppresses tumor growth. PMID: 24409098
    12. IFN-lambda receptor 1 expression is induced in chronic hepatitis C and correlates with the IFN-lambda3 genotype and with nonresponsiveness to IFN-alpha therapies. PMID: 24752298
    13. The rs12980275 AG/GG genotype and rs10903035 G allele remained independently associated with early pegylated interferon and ribavirin failure of hepatitis C in hepatitis C/HIV coinfected patients. PMID: 23565619
    14. Plasmacytoid dendritic cells are major producers of IFN-lambda2 (a type III interferon) in response to viral stimulation and also express functional receptors for this cytokine. PMID: 22891284
    15. No consistent association is found between IL28RA and multiple sclerosis in a Basque-Spanish population. PMID: 22386267
    16. two SNPs in IL28RA are correlated with susceptibility to HCV infection and spontaneous viral clearance, which implicates a primary role of IL28RA in the outcomes of HCV infection PMID: 21742059
    17. IFN-lambdaR1 consists of two distinct domains having fibronectin type III topology. PMID: 20934432
    18. Although the IFN-lambda1(Bac)-IFN-lambda1R1 and IFN-lambda1(Ins)-IFN-lambda1R1 complexes differed only in the nature of the expression system used for the ligand, their crystallization conditions and crystal forms were quite different PMID: 20057073
    19. study supports that IFN-lambdas do not influence every type of cell and that membrane-associated variant IFN-lambda R1 expression is not sufficient to ensure cellular sensitivity toward these cytokines. PMID: 19798076
    20. IL-28 and IL-29 interact with a heterodimeric class II cytokine receptor that consisted of IL-10 receptor beta (IL-10Rbeta) and an orphan class II receptor chain, designated IL-28Ralpha. PMID: 12469119
    21. distinct receptor complex that is utilized by all three IFN-lambda proteins for signaling and is composed of two subunits, a receptor designated CRF2-12 (also designated as IFN-lambdaR1) and a second subunit, CRF2-4 (also known as IL-10R2) PMID: 12483210
    22. interleukin (IL)-28 receptor tyrosine residues have roles in antiviral and antiproliferative activity of IL-29/interferon-lambda 1 PMID: 15166220
    23. Results suggested that the g.32349 G>A polymorphism of IL-28RA might be associated with susceptibility to allergic rhinitis. PMID: 16819289

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Type II cytokine receptor family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 18584

    OMIM: 607404

    KEGG: hsa:163702

    STRING: 9606.ENSP00000327824

    UniGene: Hs.221375