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  4. CD45 Monoclonal Antibody

CD45 Monoclonal Antibody

  • 货号：
    CSB-MA019049A0m
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, THP-1 whole cell lysate
      All lanes CD45 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 148, 132, 143, 141, 139, 136 KDa
      Observed band size: 180-250 KDa
      Exposure time:15min  
    • Western Blot
      Positive WB detected in: U937 whole cell lysate
      All lanes CD45 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 148, 132, 143, 141, 139, 136 KDa
      Observed band size: 180-250 KDa
      Exposure time:5min  
    • Western Blot
      Positive WB detected in: THP-1whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg All lanes: CD45 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 148, 132, 143, 141, 139, 136 KDa
      Observed band size: 180-250 KDa
      Exposure time:15min
    • Western Blot
      Positive WB detected in: 20μg THP-1 whole cell lysate CD45 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 148, 132, 143, 141, 139, 136 KDa
      Observed band size: 180-250 KDa
      Exposure time:15min
    • IHC image of CSB-MA019049A0m diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-MA019049A0m diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Jurkat cells with CSB-MA019049A0m at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Immunofluorescence staining of Raji cells with CSB-MA019049A0m at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Immunofluorescence staining of U937 cells with CSB-MA019049A0m at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Overlay histogram showing Jurkat cells stained with CSB-MA019049A0m (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
    • Overlay histogram showing Raji cells stained with CSB-MA019049A0m (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    This is a mouse monoclonal CD45 antibody generated by immunizing mice with the recombinant human receptor-type tyrosine-protein phosphatase C protein (24-575AA). Its isotype is IgG2b. This CD45 monoclonal antibody is recommended for ELISA, WB, IHC, IF, and FC applications and can detect CD45 protein of human origin. This monoclonal CD45 antibody is purified using protein A and reaches up to 95% in purity.

    The target CD45 protein is an abundant leukocyte cell surface glycoprotein exclusively upon cells of the hematopoietic system. CD45 has been considered as a useful biomarker for the differential diagnosis of undifferentiated lymphoma due to its leukocyte-specific distribution. Alternative splicing makes CD45 protein present multiple isoforms, which share identical transmembrane and cytoplasmic domains but differ in extracellular domains. CD45 glycoprotein plays a crucial role in the antigen-stimulated proliferation of T lymphocytes and thymic development. It is also an essential regulator of T and B cell antigen receptor-mediated activation. As a receptor-type protein tyrosine phosphatase (PTP), CD45 can either positively or negatively regulate Src-family protein tyrosine kinase (PTK) activity in vivo.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) PTPRC Monoclonal Antibody antibody
  • Uniprot No.：
    P08575
  • 基因名：
  • 别名：
    B220 antibody; CD 45 antibody; CD45 antibody; CD45 antigen antibody; CD45R antibody; GP180 antibody; L-CA antibody; LCA antibody; Leukocyte common antigen antibody; loc antibody; Ly-5 antibody; LY5 antibody; Ly5, homolog of antibody; Lyt-4 antibody; OTTHUMP00000033813 antibody; OTTHUMP00000033816 antibody; OTTHUMP00000033817 antibody; OTTHUMP00000038574 antibody; Protein tyrosine phosphatase receptor type c polypeptide antibody; Protein tyrosine phosphatase, receptor type C antibody; protein tyrosine phosphatase, receptor type, C antibody; Protein tyrosine phosphatase, receptor type, c polypeptide antibody; Ptprc antibody; PTPRC_HUMAN antibody; Receptor-type tyrosine-protein phosphatase C antibody; T200 antibody; T200 glycoprotein antibody; T200 leukocyte common antigen antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Receptor-type tyrosine-protein phosphatase C protein (24-575AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal Antibody
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    17H9D4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:2000-1:32000
    IHC 1:200-1:600
    IF 1:100-1:300
    FC 1:200-1:600
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.; (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
  • 基因功能参考文献：
    1. Overall, this study reveals the heterogeneity of CD4 effector memory T cells expressing CD45RA and provides insights into T-cell responses against dengue virus and other viral pathogens. PMID: 29133794
    2. Five-year disease-free survival for patients with a high transcriptional expression of CD45 (n = 107) was 62.4% and for patients with a low expression (n = 53) it was 36.2% (P = 0.003). Patients with a high expression of CD45 had a better local recurrence-free survival and disease-specific survival. PMID: 29177949
    3. CD45 is a regulator of IL-2 synergy in the NKG2D-mediated activation of immature human NK cells PMID: 28655861
    4. TCR phosphorylation negatively correlates with TCR-CD45 separation. PMID: 29467364
    5. Our results demonstrate that the expression of CD163 and CD206 in monocytes is modulated by LPS in vitro; LPS induces CD163 expression and downregulates the spontaneously increased expression of CD206 PMID: 25914252
    6. Our results show that the kinetic strategy used in this study may be a simple and easy approach to assess by RT-FCM the reaction between NO and superoxide ion in whole blood monocytes..The no-wash, no-lyse staining protocol with CD45-KO and CD14-PB allows to differentiate clearly and to gate in the monocyte population in near-physiological conditions PMID: 25758468
    7. regulatory effect of the mannose receptor (MR) was mediated by a direct interaction with CD45 on the T cell, inhibiting its phosphatase activity, which resulted in up-regulation of CTLA-4 and the induction of T-cell tolerance. Inhibition of CD45 prevented expression of B-cell lymphoma 6 (Bcl-6), a transcriptional inhibitor that directly bound the CTLA-4 promoter and regulated its activity PMID: 27601670
    8. pUL11 induces IL-10 producing T cells as a result of pUL11 binding to the CD45 phosphatase on T cells. PMID: 28628650
    9. Expression of IL10R subunits within the leukocyte population (CD45(+) cells) was significantly higher in primary brain tumors than in metastases. PMID: 28982901
    10. As CD45 expression vs. SSc is routinely measured in the diagnostics of acute leukemias. PMID: 26415521
    11. A phosphosite within the SH2 Domain of Lck regulates its activation by CD45. A negative feedback loop that responds to signaling events tunes active Lck amounts and TCR sensitivity. PMID: 28735895
    12. C77G is not associated with ovarian cancer in the Norwegian population. However, it is possibly associated with a less aggressive cancer type. PMID: 28759630
    13. Our findings suggest that CD45 is a key regulator of BCR-signaling thresholds mediated by T-cell help PMID: 27056269
    14. we demonstrate for the first time the physiological existence of ct-CD45 in human plasma and show that it may be an extrinsic factor contributing to the maintenance of human T-cell quiescence. PMID: 27718235
    15. Our findings suggest that if w/h ratio on SSC versus CD45 plot is less than 1.6, AML may be considered, and if it is more than 1.6, ALL may be diagnosed. Using morphometric analysis of the blast cluster on SSC versus CD45, it was possible to distinguish between ALL and AML, and their subtypes. PMID: 27748273
    16. Use of the common leukocyte marker CD45 increases the sensitivity of the diagnosis of lymphocytic myocarditis. PMID: 28025077
    17. Review/Meta-analysis: Rheumatoid arthritis patients with PTPRC rs10919563 A allele show a poor response to anti-TNF therapy. PMID: 27074847
    18. Data suggest that CD41 and CD45 expression marked the onset of haemangioblastoma (HB) neovascularisation and the stepwise development of the angioformative period, and also the underlying therapeutic targets of anti-vascular treatment. PMID: 26468019
    19. PTPRC has become the most replicated genetic biomarker of response to TNF inhibitors PMID: 25896535
    20. CD45RO in tumor-infiltrating lymphocytes was found to be a positive prognostic factor in squamous non-small cell lung cancer. PMID: 26678911
    21. CD45RO+ memory T-cells produce IL-17 in patients with atherosclerosis. PMID: 26667768
    22. Data show the structural basis for, and the potent signaling effects of, local CD45 antigen and kinase segregation. PMID: 26998761
    23. a CD45+/CD19 - cell population in bone marrow aspirates correlated with the clinical outcome of patients with mantle cell lymphoma. PMID: 25739938
    24. High CD45 expression is associated with multiple myeloma. PMID: 26994849
    25. C77G T(reg) showed diminished upregulation of activation markers, lower phosphorylation of p56(lck)(Y505) and a reduced proliferative potential when stimulated with anti-TcR or anti-TcR plus CD28 mAb suggesting decreased responsiveness to activating stimuli. Data suggest that the changes in CD45 isoform combination resulting from the C77G mutation alter the responsiveness PMID: 26355564
    26. Findings indicate that CD45 antigen(+) and c-Kit protein(+) hematopoietic cells were more abundant in muscle than in bone marrow between embryonic 14.5 and 17.5 days. PMID: 26389592
    27. CD4+CD45RO+CD25-/lowCD127+: CD4+CD45RO+CD25hiCD127-/low ratio in peripheral blood indicates heart transplant recipients at risk for cardiac allograft vasculopathy. PMID: 25539460
    28. CD45+ cells were abundant in the stroma of physiologically immature placental villi and decreased as pregnancy progressed. PMID: 25043745
    29. This study demonstrated that the copy number variations of PTPRC relate to opioid dependence. PMID: 25345593
    30. This study did not replicate the association between PTPRC and the response to anti-TNF treatment in our Southern European population. We found that TRAF1/C5 risk RA variants potentially influence anti-TNF treatment response. PMID: 25834819
    31. long noncoding RNA encoded by the natural antisense gene of CD45 contributes to the expressional regulation of the CD45RO splicing variant via recruitment of DNA methyltransferase and histone modification modulators specific to the sense gene CD45 PMID: 25381328
    32. In T-cells, cholesterol-dependent domains function in the regulation of the Src family kinase Lck (p56lck) by sequestering Lck from its activator CD45. (Review) PMID: 25658353
    33. Patients with the presence of CD8- and CD45RO-positive T cells in bone marrow demonstrated better survival of gastric cancer patients than those with the absence of these cells in bone marrow. PMID: 25804232
    34. Low expression of CD39(+) /CD45RA(+) on regulatory T cells (Treg ) cells in type 1 diabetic children in contrast to high expression of CD101(+) /CD129(+) on Treg cells in children with coeliac disease. PMID: 25421756
    35. Late-outgrowth CD45 negative endothelial progenitor cells express markers associated with pluripotency and can directly express an osteogenic phenotype under bone differentiation conditions. PMID: 25531767
    36. SLAMF7-triggered inhibition is mediated by a mechanism involving Src kinases, CD45, and SHIP-1 that is defective in MM cells PMID: 25312647
    37. Phosphatase CD45 both positively and negatively regulates T cell receptor phosphorylation in reconstituted membrane protein clusters, depending on LCK activity. PMID: 25128530
    38. Results show that CD45 PTP activity is inhibited by galectin-1 in anaplastic large cell lymphoma cell line H-ALCL. PMID: 24589677
    39. Expressing CD45 promoters containing these regions and tethered to green fluorescent protein (GFP) in a primary B-cell differentiation assay and a transplantation model resulted in high levels of GFP in lymphoid, myeloid, and nucleated erythroid cells PMID: 24852660
    40. The rare sub-population of CD45(-)/Lin(-)/SSEA-4(+) VSEL stem cells survived after Hespan sedimentation. PMID: 24364909
    41. Hematopoietic cell marker CD45 is expressed in hepatic progenitor cells. PMID: 24396288
    42. CD45RA-Foxp3high Tregs increase in the peripheral circulation of head and neck squamous cell carcinoma patients. PMID: 24761979
    43. the regulation of alternative splicing in CD45 by IkappaBL was independent from the kinase activity of CLK1. PMID: 23953137
    44. high CD45 surface expression is associated with a poor prognosis in BCP-ALL as well as in T-ALL. PMID: 23911702
    45. PTPRC/CD45 is down-regulated in leukemogenic tyrosine kinase expressing cells. PMID: 23997015
    46. [review] Circulating CD34+/KDR+/CD45dim endothelial progenitor cells have great potential as biomarkers of vasculogenesis and endothelial repair wjen research protocols of in vitro culture and flow cytometry are included. PMID: 23171577
    47. Heterogeneity within the Lin(-)CD45(-) cell fraction is the likely explanation for differences in the hUCB cell populations. PMID: 23840798
    48. Galectin-3-induced apoptosis of Jurkat cells is regulated by both O-glycans and N-glycans on CD45. PMID: 24211831
    49. spatial regulation of Lck by CD45 and GM1 ganglioside determines the outcome of apoptotic response to Gal-1 and this local regulation may occur only upon intimate effector (Gal-1 expressing) cell-T-cell attachment. PMID: 24231767
    50. A glycosylation-dependent CD45RB epitope defines previously unacknowledged CD27-(IgM high) B cell subpopulations enriched in young children and after hematopoietic stem cell transplantation. PMID: 24211716

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  • 相关疾病：
    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); Multiple sclerosis (MS)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Receptor class 1/6 subfamily
  • 组织特异性：
    Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Is
  • 数据库链接：

    HGNC: 9666

    OMIM: 126200

    KEGG: hsa:5788

    STRING: 9606.ENSP00000356346

    UniGene: Hs.654514